Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste
In this study, food wastes such as waste bread, savory, waste cakes, cafeteria waste, fruits, vegetables and potatoes were used as sole substrate for glucoamylase production by solid state fermentation. Response surface methodology was employed to optimize the fermentation conditions for improving t...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Green Wave Publishing of Canada
2014-08-01
|
Series: | Biofuel Research Journal |
Subjects: | |
Online Access: | http://www.biofueljournal.com/pdf_6167_e1689aa3369011f0b8b2bd9e15ca629c.html |
id |
doaj-bf1fb261a2a64f96be50053a7ea7b876 |
---|---|
record_format |
Article |
spelling |
doaj-bf1fb261a2a64f96be50053a7ea7b8762020-11-24T20:41:28ZengGreen Wave Publishing of CanadaBiofuel Research Journal2292-87822292-87822014-08-0113981056167Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food wasteEsra Uçkun Kiran0Antoine P. Trzcinski1Yu Liu2Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.|Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.In this study, food wastes such as waste bread, savory, waste cakes, cafeteria waste, fruits, vegetables and potatoes were used as sole substrate for glucoamylase production by solid state fermentation. Response surface methodology was employed to optimize the fermentation conditions for improving the production of high activity enzyme. It was found that waste cake was the best substrate for glucoamylase production. Among all the parameters studied, glucoamylase activity was significantly affected by the initial pH and incubation time. The highest glucoamylase activity of 108.47 U/gds was achieved at initial pH of 7.9, moisture content of 69.6% wt., inoculum loading of 5.2×105 cells/gram substrate (gs) and incubation time of 6 d. The enzyme preparation could effectively digest 50% suspension of domestic food waste in 24 h with an almost complete saccharification using an enzyme dose of only 2U/g food waste at 60°C.http://www.biofueljournal.com/pdf_6167_e1689aa3369011f0b8b2bd9e15ca629c.htmlGlucoamylaseAspergillus awamoriFood wasteSaccharificationSolid state fermentationResponse Surface Methodology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Esra Uçkun Kiran Antoine P. Trzcinski Yu Liu |
spellingShingle |
Esra Uçkun Kiran Antoine P. Trzcinski Yu Liu Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste Biofuel Research Journal Glucoamylase Aspergillus awamori Food waste Saccharification Solid state fermentation Response Surface Methodology |
author_facet |
Esra Uçkun Kiran Antoine P. Trzcinski Yu Liu |
author_sort |
Esra Uçkun Kiran |
title |
Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
title_short |
Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
title_full |
Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
title_fullStr |
Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
title_full_unstemmed |
Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
title_sort |
glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste |
publisher |
Green Wave Publishing of Canada |
series |
Biofuel Research Journal |
issn |
2292-8782 2292-8782 |
publishDate |
2014-08-01 |
description |
In this study, food wastes such as waste bread, savory, waste cakes, cafeteria waste, fruits, vegetables and potatoes were used as sole substrate for glucoamylase production by solid state fermentation. Response surface methodology was employed to optimize the fermentation conditions for improving the production of high activity enzyme. It was found that waste cake was the best substrate for glucoamylase production. Among all the parameters studied, glucoamylase activity was significantly affected by the initial pH and incubation time. The highest glucoamylase activity of 108.47 U/gds was achieved at initial pH of 7.9, moisture content of 69.6% wt., inoculum loading of 5.2×105 cells/gram substrate (gs) and incubation time of 6 d. The enzyme preparation could effectively digest 50% suspension of domestic food waste in 24 h with an almost complete saccharification using an enzyme dose of only 2U/g food waste at 60°C. |
topic |
Glucoamylase Aspergillus awamori Food waste Saccharification Solid state fermentation Response Surface Methodology |
url |
http://www.biofueljournal.com/pdf_6167_e1689aa3369011f0b8b2bd9e15ca629c.html |
work_keys_str_mv |
AT esrauckunkiran glucoamylaseproductionfromfoodwastebysolidstatefermentationanditsevaluationinthehydrolysisofdomesticfoodwaste AT antoineptrzcinski glucoamylaseproductionfromfoodwastebysolidstatefermentationanditsevaluationinthehydrolysisofdomesticfoodwaste AT yuliu glucoamylaseproductionfromfoodwastebysolidstatefermentationanditsevaluationinthehydrolysisofdomesticfoodwaste |
_version_ |
1716825014124150784 |