Candida rugosa lipase alters the gastrointestinal environment in wild-type mice
Diet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans...
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doaj-bf27c27117f84201bb843f0c90c916472021-05-20T07:43:23ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-10-01130110579Candida rugosa lipase alters the gastrointestinal environment in wild-type miceAriane Menden0Davane Hall1Courtney Ann Broedlow2Teresa Darcey3Fiona Crawford4Nichole Klatt5Stefan Crynen6Michael Mullan7Ghania Ait-Ghezala8Roskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States; Open University, Walton Hall, Kents Hill, Milton-Keynes, MK7 6AA, UK; Corresponding author at: Roskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States.Roskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United StatesDivision of Surgical Outcomes and Precision Medicine Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN, 55455, United StatesRoskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United StatesRoskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States; Open University, Walton Hall, Kents Hill, Milton-Keynes, MK7 6AA, UK; James A. Haley Veterans' Hospital, 13000 Bruce B. Downs Boulevard, Tampa, FL, 33612, United StatesDivision of Surgical Outcomes and Precision Medicine Research, Department of Surgery, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN, 55455, United StatesRoskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States; Open University, Walton Hall, Kents Hill, Milton-Keynes, MK7 6AA, UKRoskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States; Open University, Walton Hall, Kents Hill, Milton-Keynes, MK7 6AA, UKRoskamp Institute, 2040 Whitfield Avenue, Sarasota, FL, 34243, United States; Open University, Walton Hall, Kents Hill, Milton-Keynes, MK7 6AA, UKDiet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond the stomach in the intestinal tract of C57BL/6 J mice and its impact on the gut microbial community and environment.We showed for the first time the movement of CRL in an active state through the mouse digestive tract by determination of intestinal CRL activity and free fatty acids concentrations. The short- and long-term administration of CRL resulted in significant alterations of the gut microbiome, favoring the growth of, for instance, Verrucomicrobia but also other species associated with normal body mass index (BMI) or butyrate expression, both considered beneficial. In addition, we showed that these changes persisted after supplementation and that gut barrier integrity was unaffected by the treatment.In conclusion, CRL can be delivered in an active state beyond the stomach and supplementation altered the murine gut microbiome favoring beneficial bacterial species, which may be of relevance in humans in healthy but also potentially in disease states.http://www.sciencedirect.com/science/article/pii/S0753332220307721Enzyme supplementCandida rugosa lipaseGut microbiomeIntestinal healthAkkermansia muciniphila |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ariane Menden Davane Hall Courtney Ann Broedlow Teresa Darcey Fiona Crawford Nichole Klatt Stefan Crynen Michael Mullan Ghania Ait-Ghezala |
spellingShingle |
Ariane Menden Davane Hall Courtney Ann Broedlow Teresa Darcey Fiona Crawford Nichole Klatt Stefan Crynen Michael Mullan Ghania Ait-Ghezala Candida rugosa lipase alters the gastrointestinal environment in wild-type mice Biomedicine & Pharmacotherapy Enzyme supplement Candida rugosa lipase Gut microbiome Intestinal health Akkermansia muciniphila |
author_facet |
Ariane Menden Davane Hall Courtney Ann Broedlow Teresa Darcey Fiona Crawford Nichole Klatt Stefan Crynen Michael Mullan Ghania Ait-Ghezala |
author_sort |
Ariane Menden |
title |
Candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
title_short |
Candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
title_full |
Candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
title_fullStr |
Candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
title_full_unstemmed |
Candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
title_sort |
candida rugosa lipase alters the gastrointestinal environment in wild-type mice |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-10-01 |
description |
Diet and commercially available supplements can significantly impact the gut microbial composition; however, the effects of supplements often lack scientific data demonstrating the effects on healthy and diseased individuals. Hence, it was investigated, whether a frequently used supplement in humans, Candida rugosa lipase (CRL), gets delivered active beyond the stomach in the intestinal tract of C57BL/6 J mice and its impact on the gut microbial community and environment.We showed for the first time the movement of CRL in an active state through the mouse digestive tract by determination of intestinal CRL activity and free fatty acids concentrations. The short- and long-term administration of CRL resulted in significant alterations of the gut microbiome, favoring the growth of, for instance, Verrucomicrobia but also other species associated with normal body mass index (BMI) or butyrate expression, both considered beneficial. In addition, we showed that these changes persisted after supplementation and that gut barrier integrity was unaffected by the treatment.In conclusion, CRL can be delivered in an active state beyond the stomach and supplementation altered the murine gut microbiome favoring beneficial bacterial species, which may be of relevance in humans in healthy but also potentially in disease states. |
topic |
Enzyme supplement Candida rugosa lipase Gut microbiome Intestinal health Akkermansia muciniphila |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220307721 |
work_keys_str_mv |
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