Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions

Derivation and Maintenance of Murine Trophoblast Stem Cells under Defined Conditions

Trophoblast stem cells (TSCs) are in vitro equivalents to the precursor cells of the placenta. TSCs are cultured in serum-rich medium with fibroblast growth factor 4, heparin, and embryonic-fibroblast-conditioned medium. Here, we developed a simple medium consisting of ten chemically defined ingredi...

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Bibliographic Details
Main Authors: Caroline Kubaczka, Claire Senner, Marcos J. Araúzo-Bravo, Neha Sharma, Peter Kuckenberg, Astrid Becker, Andreas Zimmer, Oliver Brüstle, Michael Peitz, Myriam Hemberger, Hubert Schorle
Format: Article
Language:English
Published: Elsevier 2014-02-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671113001811

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