Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus

Abstract Background and objectives: Colonization of nasopharynx by Streptococcus Pneumoniae can lead to pneumococcal disease. This study was performed to determine the carriage rate of nasopharyngeal S.pneumoniae in adolescents and their antibiotic susceptibility and serotype prevalence in Zahedan,...

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Main Authors: M Bokaeian, H A Khazaee, M Javadi Mehr
Format: Article
Language:English
Published: Golestan University of Medical Sciences 2010-04-01
Series:Medical Laboratory Journal
Subjects:
Online Access:http://mlj.goums.ac.ir/article-1-10-en.html
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spelling doaj-bf3cd05c10ef4ad8bb37dacf553d05b42021-09-01T13:20:23ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492010-04-014100Nasopharyngeal carriage, antibiotic resistance and serotype distribution of StreptococcusM Bokaeian0H A Khazaee1M Javadi Mehr2 Assistant Professor, Dept. of Laboratory Abstract Background and objectives: Colonization of nasopharynx by Streptococcus Pneumoniae can lead to pneumococcal disease. This study was performed to determine the carriage rate of nasopharyngeal S.pneumoniae in adolescents and their antibiotic susceptibility and serotype prevalence in Zahedan, Iran Material and Methods: Nasopharyngeal specimens were obtained from 865 adolescents aged 10-19 years old of eight schools in Zahedan and then assessed by standard procedures to isolate S. Pneumoniae. The serotyping was carried out by latex agglutination test, the minimum inhibitory concentration (MIC) of penicillin, as well as other commonly used antibiotics, was determined by a broth-dilution method. Results: Pneumococci were Isolated from 15.7% [136/865, 95% confidence interval (CI) 12.3-18.9] of total samples. Of 136 samples, 119 isolates are typified by the available antisera which the most frequent ones are 1, 19A, 15C, 9V, 11A and 19F. Ninthythree pneumococcal isolates are sensitive to penicillin. The MIC values of antibiotics tested are (μg/ml): penicillin 0.01-4, cefotaxime 0.01-4, ceftriaxone 0.02-128, chloramphenicol 0.08-32, ciprofloxacin 0.06-16, erythromycin 0.01-128, tetracycline 0.08- 128 and vancomycin 0.02-1. Conclusion: A clear diversity is seen in the serotype distribution of the S. Pneumoniae isolates and most of the antibiotic resistant strains belonge to a few serotypes. Healthy adolescents in Zahedan commonly show pneumococcal carriage and antibiotic resistance. Keywords: Streptococcus Pneumoniae, nasopharyngeal carriage, penicillin resistance, serotypehttp://mlj.goums.ac.ir/article-1-10-en.htmlkeywords: streptococcus pneumoniaenasopharyngeal carriage
collection DOAJ
language English
format Article
sources DOAJ
author M Bokaeian
H A Khazaee
M Javadi Mehr
spellingShingle M Bokaeian
H A Khazaee
M Javadi Mehr
Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
Medical Laboratory Journal
keywords: streptococcus pneumoniae
nasopharyngeal carriage
author_facet M Bokaeian
H A Khazaee
M Javadi Mehr
author_sort M Bokaeian
title Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
title_short Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
title_full Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
title_fullStr Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
title_full_unstemmed Nasopharyngeal carriage, antibiotic resistance and serotype distribution of Streptococcus
title_sort nasopharyngeal carriage, antibiotic resistance and serotype distribution of streptococcus
publisher Golestan University of Medical Sciences
series Medical Laboratory Journal
issn 2538-4449
publishDate 2010-04-01
description Abstract Background and objectives: Colonization of nasopharynx by Streptococcus Pneumoniae can lead to pneumococcal disease. This study was performed to determine the carriage rate of nasopharyngeal S.pneumoniae in adolescents and their antibiotic susceptibility and serotype prevalence in Zahedan, Iran Material and Methods: Nasopharyngeal specimens were obtained from 865 adolescents aged 10-19 years old of eight schools in Zahedan and then assessed by standard procedures to isolate S. Pneumoniae. The serotyping was carried out by latex agglutination test, the minimum inhibitory concentration (MIC) of penicillin, as well as other commonly used antibiotics, was determined by a broth-dilution method. Results: Pneumococci were Isolated from 15.7% [136/865, 95% confidence interval (CI) 12.3-18.9] of total samples. Of 136 samples, 119 isolates are typified by the available antisera which the most frequent ones are 1, 19A, 15C, 9V, 11A and 19F. Ninthythree pneumococcal isolates are sensitive to penicillin. The MIC values of antibiotics tested are (μg/ml): penicillin 0.01-4, cefotaxime 0.01-4, ceftriaxone 0.02-128, chloramphenicol 0.08-32, ciprofloxacin 0.06-16, erythromycin 0.01-128, tetracycline 0.08- 128 and vancomycin 0.02-1. Conclusion: A clear diversity is seen in the serotype distribution of the S. Pneumoniae isolates and most of the antibiotic resistant strains belonge to a few serotypes. Healthy adolescents in Zahedan commonly show pneumococcal carriage and antibiotic resistance. Keywords: Streptococcus Pneumoniae, nasopharyngeal carriage, penicillin resistance, serotype
topic keywords: streptococcus pneumoniae
nasopharyngeal carriage
url http://mlj.goums.ac.ir/article-1-10-en.html
work_keys_str_mv AT mbokaeian nasopharyngealcarriageantibioticresistanceandserotypedistributionofstreptococcus
AT hakhazaee nasopharyngealcarriageantibioticresistanceandserotypedistributionofstreptococcus
AT mjavadimehr nasopharyngealcarriageantibioticresistanceandserotypedistributionofstreptococcus
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