Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering
The integration of surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) has attracted increasing interest and is highly probable to improve the sensitivity and reproducibility of spectroscopic investigations in biomedical fields. In this work, dual-mode SERS and SEF hiera...
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doaj-bf449105fdc64cb3bbfc23ed798fec012020-11-25T00:22:51ZengMDPI AGNanomaterials2079-49912019-06-019791610.3390/nano9070916nano9070916Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence ScatteringLibin Lu0Jiaru Zhang1Lishi Jiao2Yingchun Guan3School of Mechanical Engineering & Automation, Beihang University, Beijing 100191, ChinaSchool of Mechanical Engineering & Automation, Beihang University, Beijing 100191, ChinaSchool of Mechanical and Aerospace, Nanyang Technological University, Singapore 639798, SingaporeSchool of Mechanical Engineering & Automation, Beihang University, Beijing 100191, ChinaThe integration of surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) has attracted increasing interest and is highly probable to improve the sensitivity and reproducibility of spectroscopic investigations in biomedical fields. In this work, dual-mode SERS and SEF hierarchical structures have been developed on a single bio-metallic substrate. The hierarchical structure was composed of micro-grooves, nano-particles, and nano-ripples. The crystal violet was selected as reporter molecule and both the intensity of Raman and fluorescence signals were enhanced because of the dual-mode SERS−SEF phenomena with enhancement factors (EFs) of 7.85 × 10<sup>5</sup> and 14.32, respectively. The Raman and fluorescence signals also exhibited good uniformity with the relative standard deviation value of 2.46% and 5.15%, respectively. Moreover, the substrate exhibited high sensitivity with the limits of detection (LOD) as low as 1 × 10<sup>−11</sup> mol/L using Raman spectroscopy and 1 × 10<sup>−10</sup> mol/L by fluorescence spectroscopy. The combined effect of surface plasmon resonance and “hot spots” induced by the hierarchical laser induced periodical surface structures (LIPSS) was mainly contributed to the enhancement of Raman and fluorescence signal. We propose that the integration of SERS and SEF in a single bio-metallic substrate is promising to improve the sensitivity and reproducibility of detection in biomedical investigations.https://www.mdpi.com/2079-4991/9/7/916hierarchical LIPSSsurface-enhanced Raman scatteringsurface-enhanced fluorescencebio-metallic substratecrystal violet |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Libin Lu Jiaru Zhang Lishi Jiao Yingchun Guan |
spellingShingle |
Libin Lu Jiaru Zhang Lishi Jiao Yingchun Guan Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering Nanomaterials hierarchical LIPSS surface-enhanced Raman scattering surface-enhanced fluorescence bio-metallic substrate crystal violet |
author_facet |
Libin Lu Jiaru Zhang Lishi Jiao Yingchun Guan |
author_sort |
Libin Lu |
title |
Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering |
title_short |
Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering |
title_full |
Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering |
title_fullStr |
Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering |
title_full_unstemmed |
Large-Scale Fabrication of Nanostructure on Bio-Metallic Substrate for Surface Enhanced Raman and Fluorescence Scattering |
title_sort |
large-scale fabrication of nanostructure on bio-metallic substrate for surface enhanced raman and fluorescence scattering |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2019-06-01 |
description |
The integration of surface-enhanced Raman scattering (SERS) and surface-enhanced fluorescence (SEF) has attracted increasing interest and is highly probable to improve the sensitivity and reproducibility of spectroscopic investigations in biomedical fields. In this work, dual-mode SERS and SEF hierarchical structures have been developed on a single bio-metallic substrate. The hierarchical structure was composed of micro-grooves, nano-particles, and nano-ripples. The crystal violet was selected as reporter molecule and both the intensity of Raman and fluorescence signals were enhanced because of the dual-mode SERS−SEF phenomena with enhancement factors (EFs) of 7.85 × 10<sup>5</sup> and 14.32, respectively. The Raman and fluorescence signals also exhibited good uniformity with the relative standard deviation value of 2.46% and 5.15%, respectively. Moreover, the substrate exhibited high sensitivity with the limits of detection (LOD) as low as 1 × 10<sup>−11</sup> mol/L using Raman spectroscopy and 1 × 10<sup>−10</sup> mol/L by fluorescence spectroscopy. The combined effect of surface plasmon resonance and “hot spots” induced by the hierarchical laser induced periodical surface structures (LIPSS) was mainly contributed to the enhancement of Raman and fluorescence signal. We propose that the integration of SERS and SEF in a single bio-metallic substrate is promising to improve the sensitivity and reproducibility of detection in biomedical investigations. |
topic |
hierarchical LIPSS surface-enhanced Raman scattering surface-enhanced fluorescence bio-metallic substrate crystal violet |
url |
https://www.mdpi.com/2079-4991/9/7/916 |
work_keys_str_mv |
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