Adaptive sequence evolution in a color gene involved in the formation of the characteristic egg-dummies of male haplochromine cichlid fishes

• Main Authors: Braasch Ingo, Salzburger Walter, Meyer Axel
• Format: Article
• Language: English
• Published: BMC 2007-11-01
• Series: BMC Biology
• Online Access: http://www.biomedcentral.com/1741-7007/5/51

<p>Abstract</p> <p>Background</p> <p>The exceptionally diverse species flocks of cichlid fishes in East Africa are prime examples of parallel adaptive radiations. About 80% of East Africa's more than 1 800 endemic cichlid species, and all species of the flocks of Lakes Victoria and Malawi, belong to a particularly rapidly evolving lineage, the haplochromines. One characteristic feature of the haplochromines is their possession of egg-dummies on the males' anal fins. These egg-spots mimic real eggs and play an important role in the mating system of these maternal mouthbrooding fish.</p> <p>Results</p> <p>Here, we show that the egg-spots of haplochromines are made up of yellow pigment cells, xanthophores, and that a gene coding for a type III receptor tyrosine kinase, <it>colony-stimulating factor 1 receptor a </it>(<it>csf1ra</it>), is expressed in egg-spot tissue. Molecular evolutionary analyses reveal that the extracellular ligand-binding and receptor-interacting domain of <it>csf1ra </it>underwent adaptive sequence evolution in the ancestral lineage of the haplochromines, coinciding with the emergence of egg-dummies. We also find that <it>csf1ra </it>is expressed in the egg-dummies of a distantly related cichlid species, the ectodine cichlid <it>Ophthalmotilapia ventralis</it>, in which markings with similar functions evolved on the pelvic fin in convergence to those of the haplochromines.</p> <p>Conclusion</p> <p>We conclude that modifications of existing signal transduction mechanisms might have evolved in the haplochromine lineage in association with the origination of anal fin egg-dummies. That positive selection has acted during the evolution of a color gene that seems to be involved in the morphogenesis of a sexually selected trait, the egg-dummies, highlights the importance of further investigations of the comparative genomic basis of the phenotypic diversification of cichlid fishes.</p>