Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients
The involvement of mitochondrial dysfunction in the pathophysiology of attention-deficit hyperactivity disorder (ADHD) has been suggested in several reports. Mitochondrial DNA (mtDNA) copy number as well as methylation of the D-loop region and peroxisome-proliferator-activated receptor γ co-activato...
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doaj-bf55c582805e4c9f9d55b0a576dbdb012020-11-24T21:37:18ZengFrontiers Media S.A.Frontiers in Psychiatry1664-06402019-07-011010.3389/fpsyt.2019.00506425973Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder PatientsJohanna Inhyang Kim0Soo-Young Lee1Mira Park2Si Yeon Kim3Jae-Won Kim4Soon Ae Kim5Bung-Nyun Kim6Department of Psychiatry, Hanyang University Medical Center, Seoul, South KoreaDepartment of Pharmacology, School of Medicine, Eulji University, Daejeon, South KoreaDepartment of Preventive Medicine, School of Medicine, Eulji University, Daejeon, South KoreaDepartment of Preventive Medicine, School of Medicine, Eulji University, Daejeon, South KoreaDivision of Child and Adolescent Psychiatry, Department of Psychiatry, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Pharmacology, School of Medicine, Eulji University, Daejeon, South KoreaDivision of Child and Adolescent Psychiatry, Department of Psychiatry, Seoul National University College of Medicine, Seoul, South KoreaThe involvement of mitochondrial dysfunction in the pathophysiology of attention-deficit hyperactivity disorder (ADHD) has been suggested in several reports. Mitochondrial DNA (mtDNA) copy number as well as methylation of the D-loop region and peroxisome-proliferator-activated receptor γ co-activator-1α (PPARGC1A) are considered biomarkers for mitochondrial dysfunction. We compared the mtDNA copy number and methylation ratio of the D-loop region and PPARGC1A between ADHD patients and controls and also among ADHD subtypes. The present study included 70 subjects with ADHD and 70 age- and gender-matched healthy controls (HCs). We measured the relative mtDNA copy number in peripheral blood cells using quantitative polymerase chain reaction (qPCR), and the methylation ratio was measured using methylation-specific PCR (MSP) after bisulfite conversion. The relative mtDNA copy number was significantly higher in ADHD patients than in HCs (p = 0.028). The mtDNA methylation ratio of PPARGC1A was decreased in ADHD patients compared with HCs (p = 0.008). After adjusting for IQ level, only the mtDNA copy number differed between the ADHD and HCs (p = 0.01). There was a significant difference in the methylation ratio of PPARGC1A among ADHD subtypes. These results suggest the possible involvement of mitochondrial dysfunction in the pathophysiology of ADHD. Further large cohort studies investigating the correlation between clinical markers and biomarkers of mitochondrial dysfunction are warranted.https://www.frontiersin.org/article/10.3389/fpsyt.2019.00506/fullattention-deficit hyperactivity disorder (ADHD)mitochondrial dysfunctionmitochondrial DNA (mtDNA)peroxisome-proliferator-activated receptor γ co-activator-1α,DNA methylation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Johanna Inhyang Kim Soo-Young Lee Mira Park Si Yeon Kim Jae-Won Kim Soon Ae Kim Bung-Nyun Kim |
spellingShingle |
Johanna Inhyang Kim Soo-Young Lee Mira Park Si Yeon Kim Jae-Won Kim Soon Ae Kim Bung-Nyun Kim Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients Frontiers in Psychiatry attention-deficit hyperactivity disorder (ADHD) mitochondrial dysfunction mitochondrial DNA (mtDNA) peroxisome-proliferator-activated receptor γ co-activator-1α,DNA methylation |
author_facet |
Johanna Inhyang Kim Soo-Young Lee Mira Park Si Yeon Kim Jae-Won Kim Soon Ae Kim Bung-Nyun Kim |
author_sort |
Johanna Inhyang Kim |
title |
Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients |
title_short |
Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients |
title_full |
Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients |
title_fullStr |
Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients |
title_full_unstemmed |
Peripheral Mitochondrial DNA Copy Number is Increased in Korean Attention-Deficit Hyperactivity Disorder Patients |
title_sort |
peripheral mitochondrial dna copy number is increased in korean attention-deficit hyperactivity disorder patients |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Psychiatry |
issn |
1664-0640 |
publishDate |
2019-07-01 |
description |
The involvement of mitochondrial dysfunction in the pathophysiology of attention-deficit hyperactivity disorder (ADHD) has been suggested in several reports. Mitochondrial DNA (mtDNA) copy number as well as methylation of the D-loop region and peroxisome-proliferator-activated receptor γ co-activator-1α (PPARGC1A) are considered biomarkers for mitochondrial dysfunction. We compared the mtDNA copy number and methylation ratio of the D-loop region and PPARGC1A
between ADHD patients and controls and also among ADHD subtypes. The present study included 70 subjects with ADHD and 70 age- and gender-matched healthy controls (HCs). We measured the relative mtDNA copy number in peripheral blood cells using quantitative polymerase chain reaction (qPCR), and the methylation ratio was measured using methylation-specific PCR (MSP) after bisulfite conversion. The relative mtDNA copy number was significantly higher in ADHD patients than in HCs (p = 0.028). The mtDNA methylation ratio of PPARGC1A was decreased in ADHD patients compared with HCs (p = 0.008). After adjusting for IQ level, only the mtDNA copy number differed between the ADHD and HCs (p = 0.01). There was a significant difference in the methylation ratio of PPARGC1A
among ADHD subtypes. These results suggest the possible involvement of mitochondrial dysfunction in the pathophysiology of ADHD. Further large cohort studies investigating the correlation between clinical markers and biomarkers of mitochondrial dysfunction are warranted. |
topic |
attention-deficit hyperactivity disorder (ADHD) mitochondrial dysfunction mitochondrial DNA (mtDNA) peroxisome-proliferator-activated receptor γ co-activator-1α,DNA methylation |
url |
https://www.frontiersin.org/article/10.3389/fpsyt.2019.00506/full |
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