A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring
In this paper, we used sputtering and screen-printing technology to develop a sensitive potentiometric ascorbic acid (AA) biosensor with the magnetic beads-ascorbate oxidase/graphene oxide/zinc oxide (MBs-AO/GO/ZnO) membrane-based arrayed screen-printed electrodes (SPEs). The morphology of membranes...
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doaj-bf5957a9998c4eaebcfb6f51c76e92aa2021-04-05T17:04:24ZengIEEEIEEE Access2169-35362019-01-01710596210597210.1109/ACCESS.2019.29317738779626A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote MonitoringJung-Chuan Chou0https://orcid.org/0000-0002-8829-3404Si-Hong Lin1Po-Yu Kuo2https://orcid.org/0000-0003-2791-9642Chih-Hsien Lai3Yu-Hsun Nien4Tsu-Yang Lai5Tzu-Yu Su6Graduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Electronic Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanGraduate School of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Douliu, TaiwanIn this paper, we used sputtering and screen-printing technology to develop a sensitive potentiometric ascorbic acid (AA) biosensor with the magnetic beads-ascorbate oxidase/graphene oxide/zinc oxide (MBs-AO/GO/ZnO) membrane-based arrayed screen-printed electrodes (SPEs). The morphology of membranes was characterized by using a scanning probe microscope (SPM) and a field-emission scanning electron microscope (FE-SEM). The sensing characteristics were analyzed via the voltage-time (V-T) measurement system. The potentiometric arrayed AA biosensor showed the exceptional average sensitivity of 70.68 mV/decade over a wide linear range of AA concentration (7.8125 μM-2 mM), the short response time of 25 s, the lower limit of detection (LOD) of 0.04 μM, and the excellent selectivity. Furthermore, we investigated other analytical parameters of the potentiometric arrayed AA biosensor, such as the temperature effects, the lifetime, and the average sensitivity under microfluidic flow. The optimal average sensitivity of the biosensor integrated with the microfluidic framework was 72.21 mV/decade under microfluidic flow. Finally, the biosensor was applied to the remote AA detection by using the wireless sensing system. The results indicated that the average sensitivity of the potentiometric arrayed AA biosensor based on MBs-AO/GO/ZnO was 77.38 mV/decade during the monitoring in a long distance.https://ieeexplore.ieee.org/document/8779626/Zinc oxide (ZnO)graphene oxide (GO)magnetic beads (MBs)ascorbic acid (AA)screen-printed electrodes (SPEs)remote monitoring |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung-Chuan Chou Si-Hong Lin Po-Yu Kuo Chih-Hsien Lai Yu-Hsun Nien Tsu-Yang Lai Tzu-Yu Su |
spellingShingle |
Jung-Chuan Chou Si-Hong Lin Po-Yu Kuo Chih-Hsien Lai Yu-Hsun Nien Tsu-Yang Lai Tzu-Yu Su A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring IEEE Access Zinc oxide (ZnO) graphene oxide (GO) magnetic beads (MBs) ascorbic acid (AA) screen-printed electrodes (SPEs) remote monitoring |
author_facet |
Jung-Chuan Chou Si-Hong Lin Po-Yu Kuo Chih-Hsien Lai Yu-Hsun Nien Tsu-Yang Lai Tzu-Yu Su |
author_sort |
Jung-Chuan Chou |
title |
A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring |
title_short |
A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring |
title_full |
A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring |
title_fullStr |
A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring |
title_full_unstemmed |
A Sensitive Potentiometric Biosensor Using MBs-AO/GO/ZnO Membranes-Based Arrayed Screen-Printed Electrodes for AA Detection and Remote Monitoring |
title_sort |
sensitive potentiometric biosensor using mbs-ao/go/zno membranes-based arrayed screen-printed electrodes for aa detection and remote monitoring |
publisher |
IEEE |
series |
IEEE Access |
issn |
2169-3536 |
publishDate |
2019-01-01 |
description |
In this paper, we used sputtering and screen-printing technology to develop a sensitive potentiometric ascorbic acid (AA) biosensor with the magnetic beads-ascorbate oxidase/graphene oxide/zinc oxide (MBs-AO/GO/ZnO) membrane-based arrayed screen-printed electrodes (SPEs). The morphology of membranes was characterized by using a scanning probe microscope (SPM) and a field-emission scanning electron microscope (FE-SEM). The sensing characteristics were analyzed via the voltage-time (V-T) measurement system. The potentiometric arrayed AA biosensor showed the exceptional average sensitivity of 70.68 mV/decade over a wide linear range of AA concentration (7.8125 μM-2 mM), the short response time of 25 s, the lower limit of detection (LOD) of 0.04 μM, and the excellent selectivity. Furthermore, we investigated other analytical parameters of the potentiometric arrayed AA biosensor, such as the temperature effects, the lifetime, and the average sensitivity under microfluidic flow. The optimal average sensitivity of the biosensor integrated with the microfluidic framework was 72.21 mV/decade under microfluidic flow. Finally, the biosensor was applied to the remote AA detection by using the wireless sensing system. The results indicated that the average sensitivity of the potentiometric arrayed AA biosensor based on MBs-AO/GO/ZnO was 77.38 mV/decade during the monitoring in a long distance. |
topic |
Zinc oxide (ZnO) graphene oxide (GO) magnetic beads (MBs) ascorbic acid (AA) screen-printed electrodes (SPEs) remote monitoring |
url |
https://ieeexplore.ieee.org/document/8779626/ |
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