Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes
Nanoparticle-assembled hydroxyapatite (HA) hollow microspheres have a high surface area and are convenient to handle, owing to their characteristic structure. In this study we characterized the protein adsorption of HA hollow microspheres prepared from CaCl2 and K2HPO4 by a water-in-oil-in-water (W/...
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doaj-bf6cef1a8e14460f9b80ec403bbd3a0c2020-11-25T02:20:02ZengElsevierHeliyon2405-84402019-04-0154e01490Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranesTakaaki Tanaka0Yoshihiko Takai1Akifumi Nagase2Kazuki Teraguchi3Hiromi Minbu4Akihito Ochiai5Isao Kimura6Masayuki Taniguchi7Department of Materials Science and Technology, Niigata University, Niigata 950-2181, Japan; Corresponding author.Department of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanDepartment of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanDepartment of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanDepartment of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanDepartment of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanDepartment of Chemistry and Chemical Engineering, Niigata University, Niigata 950-2181, JapanDepartment of Materials Science and Technology, Niigata University, Niigata 950-2181, JapanNanoparticle-assembled hydroxyapatite (HA) hollow microspheres have a high surface area and are convenient to handle, owing to their characteristic structure. In this study we characterized the protein adsorption of HA hollow microspheres prepared from CaCl2 and K2HPO4 by a water-in-oil-in-water (W/O/W) emulsion method assisted by two surfactants: Span 80 and Tween 20. The HA hollow microspheres adsorbed bovine serum albumin, bovine γ-globulin, equine skeletal muscle myoglobin, and chicken egg white lysozyme in 10 mM sodium phosphate buffer (pH 6.8) in a Langmuir-type adsorption and desorbed the proteins in 800 mM sodium phosphate buffer (pH 6.8). The maximum adsorbed amounts of the HA hollow microspheres were 7.5–9.0 times higher than those of the microrods with a similar size range. The composite membranes of the HA microspheres and the poly(l-lactic acid) (PLLA) microporous membranes exhibited a high adsorption capacity for γ-globulin.http://www.sciencedirect.com/science/article/pii/S2405844018379921Materials scienceMaterials chemistryPhysical chemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takaaki Tanaka Yoshihiko Takai Akifumi Nagase Kazuki Teraguchi Hiromi Minbu Akihito Ochiai Isao Kimura Masayuki Taniguchi |
spellingShingle |
Takaaki Tanaka Yoshihiko Takai Akifumi Nagase Kazuki Teraguchi Hiromi Minbu Akihito Ochiai Isao Kimura Masayuki Taniguchi Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes Heliyon Materials science Materials chemistry Physical chemistry |
author_facet |
Takaaki Tanaka Yoshihiko Takai Akifumi Nagase Kazuki Teraguchi Hiromi Minbu Akihito Ochiai Isao Kimura Masayuki Taniguchi |
author_sort |
Takaaki Tanaka |
title |
Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes |
title_short |
Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes |
title_full |
Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes |
title_fullStr |
Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes |
title_full_unstemmed |
Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes |
title_sort |
protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with plla microporous membranes |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-04-01 |
description |
Nanoparticle-assembled hydroxyapatite (HA) hollow microspheres have a high surface area and are convenient to handle, owing to their characteristic structure. In this study we characterized the protein adsorption of HA hollow microspheres prepared from CaCl2 and K2HPO4 by a water-in-oil-in-water (W/O/W) emulsion method assisted by two surfactants: Span 80 and Tween 20. The HA hollow microspheres adsorbed bovine serum albumin, bovine γ-globulin, equine skeletal muscle myoglobin, and chicken egg white lysozyme in 10 mM sodium phosphate buffer (pH 6.8) in a Langmuir-type adsorption and desorbed the proteins in 800 mM sodium phosphate buffer (pH 6.8). The maximum adsorbed amounts of the HA hollow microspheres were 7.5–9.0 times higher than those of the microrods with a similar size range. The composite membranes of the HA microspheres and the poly(l-lactic acid) (PLLA) microporous membranes exhibited a high adsorption capacity for γ-globulin. |
topic |
Materials science Materials chemistry Physical chemistry |
url |
http://www.sciencedirect.com/science/article/pii/S2405844018379921 |
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