Human infection with Plasmodium knowlesi on the Laos-Vietnam border
Abstract Background Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonot...
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doaj-bf9bb977dce9442fa4c3d016e27d68732020-11-25T02:28:18ZengBMCTropical Medicine and Health1349-41472018-09-014611610.1186/s41182-018-0116-7Human infection with Plasmodium knowlesi on the Laos-Vietnam borderTiengkham Pongvongsa0Richard Culleton1Hoang Ha2Le Thanh3Panom Phongmany4Ron P. Marchand5Satoru Kawai6Kazuhiko Moji7Shusuke Nakazawa8Yoshimasa Maeno9Savannakhet Provincial Health DepartmentMalaria Unit, Department of Pathology, Institute of Tropical Medicine, Nagasaki UniversityFaculty of Public Health, Da Nang University of Medical Technology and PharmacyMalaria-Parasitology and Entomology Department, Preventive Medicine Center of Quang Tri provinceSavannakhet Provincial Health DepartmentKhanh Phu Malaria Research Unit, Medical Committee Netherlands-Viet NamLaboratory of Tropical Medicine and Parasitology, Dokkyo Medical UniversityGraduate School of Tropical Medicine & Global Health, Nagasaki UniversityDepartment of Protozoology, Institute of Tropical Medicine, Nagasaki UniversityDepartment of Virology and Parasitology, Fujita Health University School of MedicineAbstract Background Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries.http://link.springer.com/article/10.1186/s41182-018-0116-7Plasmodium knowlesiBorder malariaLaosVietnamGreater Mekong regionForest |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiengkham Pongvongsa Richard Culleton Hoang Ha Le Thanh Panom Phongmany Ron P. Marchand Satoru Kawai Kazuhiko Moji Shusuke Nakazawa Yoshimasa Maeno |
spellingShingle |
Tiengkham Pongvongsa Richard Culleton Hoang Ha Le Thanh Panom Phongmany Ron P. Marchand Satoru Kawai Kazuhiko Moji Shusuke Nakazawa Yoshimasa Maeno Human infection with Plasmodium knowlesi on the Laos-Vietnam border Tropical Medicine and Health Plasmodium knowlesi Border malaria Laos Vietnam Greater Mekong region Forest |
author_facet |
Tiengkham Pongvongsa Richard Culleton Hoang Ha Le Thanh Panom Phongmany Ron P. Marchand Satoru Kawai Kazuhiko Moji Shusuke Nakazawa Yoshimasa Maeno |
author_sort |
Tiengkham Pongvongsa |
title |
Human infection with Plasmodium knowlesi on the Laos-Vietnam border |
title_short |
Human infection with Plasmodium knowlesi on the Laos-Vietnam border |
title_full |
Human infection with Plasmodium knowlesi on the Laos-Vietnam border |
title_fullStr |
Human infection with Plasmodium knowlesi on the Laos-Vietnam border |
title_full_unstemmed |
Human infection with Plasmodium knowlesi on the Laos-Vietnam border |
title_sort |
human infection with plasmodium knowlesi on the laos-vietnam border |
publisher |
BMC |
series |
Tropical Medicine and Health |
issn |
1349-4147 |
publishDate |
2018-09-01 |
description |
Abstract Background Border malaria in the Greater Mekong region of Southeast Asia poses a serious threat to the health of the ethnic minority populations of the region. Traditionally thought to be caused primarily by the malaria parasites Plasmodium falciparum and Plasmodium vivax, recently a zoonotic parasite, Plasmodium knowlesi, has been identified in some countries of the region. The presence of this parasite poses a challenge to malaria control programmes, as it is maintained in a zoonotic reservoir of forest-dwelling macaque monkeys. Methods A cross-sectional malaria parasite species prevalence survey was conducted along the Laos-Vietnam border in the central part of the two countries. Human blood samples were collected from Savannakhet in Laos and Quang Tri in Vietnam between August and October 2010 and assayed for the presence of human malaria parasite species and P. knowlesi. A PCR targeting the 18S small subunit ribosomal RNA gene and circumsporozoite protein gene was used for Plasmodium species identification. Results Nine cases of P. knowlesi were detected by PCR in blood samples from the Laos side and three from the Vietnam side. All P. knowlesi infections were found in co-infection with P. vivax, with some triple infections of P. knowlesi, P. vivax and P. falciparum detected in Laos. Phylogenetic analysis of these parasites suggests that P. knowlesi is circulating in the Laos-Vietnam border region. Conclusion This report shows that P. knowlesi is transmited on both sides of the Vietnam-Laos border. Continued monitoring of the range and prevalence of P. knowlesi on both the sides of Laos-Vietnam border is of importance to the National Malaria Control Programmes of both countries. |
topic |
Plasmodium knowlesi Border malaria Laos Vietnam Greater Mekong region Forest |
url |
http://link.springer.com/article/10.1186/s41182-018-0116-7 |
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