Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants
Imbalanced maternal micronutrient status, poor placentation, and oxidative stress are associated with greater risk of pregnancy complications, which impact mother and offspring health. As selenium, iodine, and copper are essential micronutrients with key roles in antioxidant systems, this study inve...
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doaj-bfc02fdc02c54217b1ed35553b21a2572021-03-01T00:04:06ZengMDPI AGNutrients2072-66432021-02-011380080010.3390/nu13030800Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta ExplantsNahal Habibi0Agatha Labrinidis1Shalem Yiner-Lee Leemaqz2Tanja Jankovic-Karasoulos3Dylan McCullough4Jessica A. Grieger5Sarah Gilbert6Carmela Ricciardelli7Shao Jia Zhou8Anthony V. Perkins9Claire T. Roberts10Tina Bianco-Miotto11School of Agriculture, Food and Wine, Waite Research Institute, and Robinson Research Institute, University of Adelaide, Adelaide, SA 5005, AustraliaAdelaide Microscopy, Division of Research and Innovation, University of Adelaide, SA 5005, AustraliaAustralia and Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaAustralia and Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaAustralia and Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaAdelaide Medical School and Robinson Research Institute, University of Adelaide, SA 5005, AustraliaAdelaide Microscopy, Division of Research and Innovation, University of Adelaide, SA 5005, AustraliaAdelaide Medical School and Robinson Research Institute, University of Adelaide, SA 5005, AustraliaSchool of Agriculture, Food and Wine, Waite Research Institute, and Robinson Research Institute, University of Adelaide, Adelaide, SA 5005, AustraliaSchool of Medical Science, Griffith University, Gold Coast Campus, Parklands Drive, Southport, QLD 9726, AustraliaAustralia and Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaSchool of Agriculture, Food and Wine, Waite Research Institute, and Robinson Research Institute, University of Adelaide, Adelaide, SA 5005, AustraliaImbalanced maternal micronutrient status, poor placentation, and oxidative stress are associated with greater risk of pregnancy complications, which impact mother and offspring health. As selenium, iodine, and copper are essential micronutrients with key roles in antioxidant systems, this study investigated their potential protective effects on placenta against oxidative stress. First trimester human placenta explants were treated with different concentrations of selenium (sodium selenite), iodine (potassium iodide), their combination or copper (copper (II) sulfate). The concentrations represented deficient, physiological, or super physiological levels. Oxidative stress was induced by menadione or antimycin. Placenta explants were collected, fixed, processed, and embedded for laser ablation inductively coupled plasma-mass spectrometry (LA ICP-MS) element imaging or immunohistochemical labelling. LA ICP-MS showed that placenta could uptake selenium and copper from the media. Sodium selenite and potassium iodide reduced DNA damage and apoptosis (<i>p</i> < 0.05). Following oxidative stress induction, a higher concentration of sodium selenite (1.6 µM) was needed to reduce DNA damage and apoptosis while both concentrations of potassium iodide (0.5 and 1 µM) were protective (<i>p</i> < 0.05). A high concentration of copper (40 µM) increased apoptosis and DNA damage but this effect was no longer significant after induction of oxidative stress. Micronutrients supplementation can increase their content within the placenta and an optimal maternal micronutrient level is essential for placenta health.https://www.mdpi.com/2072-6643/13/3/800apoptosiscopperDNA damageiodinemicronutrient supplementoxidative stress |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nahal Habibi Agatha Labrinidis Shalem Yiner-Lee Leemaqz Tanja Jankovic-Karasoulos Dylan McCullough Jessica A. Grieger Sarah Gilbert Carmela Ricciardelli Shao Jia Zhou Anthony V. Perkins Claire T. Roberts Tina Bianco-Miotto |
spellingShingle |
Nahal Habibi Agatha Labrinidis Shalem Yiner-Lee Leemaqz Tanja Jankovic-Karasoulos Dylan McCullough Jessica A. Grieger Sarah Gilbert Carmela Ricciardelli Shao Jia Zhou Anthony V. Perkins Claire T. Roberts Tina Bianco-Miotto Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants Nutrients apoptosis copper DNA damage iodine micronutrient supplement oxidative stress |
author_facet |
Nahal Habibi Agatha Labrinidis Shalem Yiner-Lee Leemaqz Tanja Jankovic-Karasoulos Dylan McCullough Jessica A. Grieger Sarah Gilbert Carmela Ricciardelli Shao Jia Zhou Anthony V. Perkins Claire T. Roberts Tina Bianco-Miotto |
author_sort |
Nahal Habibi |
title |
Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants |
title_short |
Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants |
title_full |
Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants |
title_fullStr |
Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants |
title_full_unstemmed |
Effect of Selenium and Iodine on Oxidative Stress in the First Trimester Human Placenta Explants |
title_sort |
effect of selenium and iodine on oxidative stress in the first trimester human placenta explants |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2021-02-01 |
description |
Imbalanced maternal micronutrient status, poor placentation, and oxidative stress are associated with greater risk of pregnancy complications, which impact mother and offspring health. As selenium, iodine, and copper are essential micronutrients with key roles in antioxidant systems, this study investigated their potential protective effects on placenta against oxidative stress. First trimester human placenta explants were treated with different concentrations of selenium (sodium selenite), iodine (potassium iodide), their combination or copper (copper (II) sulfate). The concentrations represented deficient, physiological, or super physiological levels. Oxidative stress was induced by menadione or antimycin. Placenta explants were collected, fixed, processed, and embedded for laser ablation inductively coupled plasma-mass spectrometry (LA ICP-MS) element imaging or immunohistochemical labelling. LA ICP-MS showed that placenta could uptake selenium and copper from the media. Sodium selenite and potassium iodide reduced DNA damage and apoptosis (<i>p</i> < 0.05). Following oxidative stress induction, a higher concentration of sodium selenite (1.6 µM) was needed to reduce DNA damage and apoptosis while both concentrations of potassium iodide (0.5 and 1 µM) were protective (<i>p</i> < 0.05). A high concentration of copper (40 µM) increased apoptosis and DNA damage but this effect was no longer significant after induction of oxidative stress. Micronutrients supplementation can increase their content within the placenta and an optimal maternal micronutrient level is essential for placenta health. |
topic |
apoptosis copper DNA damage iodine micronutrient supplement oxidative stress |
url |
https://www.mdpi.com/2072-6643/13/3/800 |
work_keys_str_mv |
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