Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing.
High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of shor...
Main Authors: | Ulrike Menzel, Victor Greiff, Tarik A Khan, Ulrike Haessler, Ina Hellmann, Simon Friedensohn, Skylar C Cook, Mark Pogson, Sai T Reddy |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4014543?pdf=render |
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