The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury
In certain small-gold mining activities in West Lombok, Indonesia, the tailings containing mercury are discharged to agricultural lands, reducing their productivity. One of the efforts to restore the land is by bioremediation of mercury, using mercury-resistant microbes. This study was aimed to isol...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Polish Society of Ecological Engineering (PTIE)
2018-03-01
|
Series: | Journal of Ecological Engineering |
Subjects: | |
Online Access: | http://www.journalssystem.com/jeeng/The-potential-of-mercury-resistant-bacteria-isolated-from-small-scale-gold-mine-tailings,83565,0,2.html |
id |
doaj-bfef2d6afc61453799eb2e20be83c933 |
---|---|
record_format |
Article |
spelling |
doaj-bfef2d6afc61453799eb2e20be83c9332020-11-24T22:10:10ZengPolish Society of Ecological Engineering (PTIE)Journal of Ecological Engineering2299-89932018-03-0119223624510.12911/22998993/8356583565The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of MercuryUmi Chasanah0Yulia Nuraini1Eko Handayanto2Postgraduate Programme, Faculty of Agriculture, Brawijaya University, Jl. Veteran No 1 Malang 65145, IndonesiaResearch Centre for Management of Degraded and Mining Lands, Faculty of Agriculture, Brawijaya University, IndonesiaResearch Centre for Management of Degraded and Mining Lands, Faculty of Agriculture, Brawijaya University, IndonesiaIn certain small-gold mining activities in West Lombok, Indonesia, the tailings containing mercury are discharged to agricultural lands, reducing their productivity. One of the efforts to restore the land is by bioremediation of mercury, using mercury-resistant microbes. This study was aimed to isolate the mercury-resistant bacteria from small-scale gold mine tailings containing mercury, and to test their capability in accumulating mercury. Bacterial isolation and identification were conducted from nutrient broth supplemented with 5 ppm HgCl 2 . The isolated bacteria were tested for mercury accumulation in the nutrient broth containing 10, 20 and 30 ppm Hg for 24 hours, and in small-scale gold mine tailing containing 41.37 ppm Hg for 2 weeks. The results showed that there were four pure isolates of mercury-resistant bacteria which were identified as Brevundimonas vesicularis , Nitrococcus mobilis , Fusobacterium aquatile and Fusobacterium necrogenes . The highest Hg accumulation from nutrient broth liquid media containing 10, 20 and 30 ppm Hg was observed for Brevundimonas vesicularis . The mercury accumulation efficiency of the four bacteria applied to small-scale gold mine tailing containing mercury was in the order of Fusobacterium aquatile (76.1%) > Brevundimonas vesicularis (75.6%) > Fusobacterium necrogenes (74.4%) > Nitrococcus mobilis (74.2%). On the basis of the Hg accumulation efficiency of more than 75%, Fusobacterium aquatile and Brevundimonas vesicularis are prospective for bioremediation of mercury-contaminated soils.http://www.journalssystem.com/jeeng/The-potential-of-mercury-resistant-bacteria-isolated-from-small-scale-gold-mine-tailings,83565,0,2.htmlmercury resistant bacteriasmall-scale gold mine tailing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Umi Chasanah Yulia Nuraini Eko Handayanto |
spellingShingle |
Umi Chasanah Yulia Nuraini Eko Handayanto The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury Journal of Ecological Engineering mercury resistant bacteria small-scale gold mine tailing |
author_facet |
Umi Chasanah Yulia Nuraini Eko Handayanto |
author_sort |
Umi Chasanah |
title |
The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury |
title_short |
The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury |
title_full |
The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury |
title_fullStr |
The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury |
title_full_unstemmed |
The Potential of Mercury-Resistant Bacteria Isolated from Small-Scale Gold Mine Tailings for Accumulation of Mercury |
title_sort |
potential of mercury-resistant bacteria isolated from small-scale gold mine tailings for accumulation of mercury |
publisher |
Polish Society of Ecological Engineering (PTIE) |
series |
Journal of Ecological Engineering |
issn |
2299-8993 |
publishDate |
2018-03-01 |
description |
In certain small-gold mining activities in West Lombok, Indonesia, the tailings containing mercury are discharged to agricultural lands, reducing their productivity. One of the efforts to restore the land is by bioremediation of mercury, using mercury-resistant microbes. This study was aimed to isolate the mercury-resistant bacteria from small-scale gold mine tailings containing mercury, and to test their capability in accumulating mercury. Bacterial isolation and identification were conducted from nutrient broth supplemented with 5 ppm HgCl 2 . The isolated bacteria were tested for mercury accumulation in the nutrient broth containing 10, 20 and 30 ppm Hg for 24 hours, and in small-scale gold mine tailing containing 41.37 ppm Hg for 2 weeks. The results showed that there were four pure isolates of mercury-resistant bacteria which were identified as Brevundimonas vesicularis , Nitrococcus mobilis , Fusobacterium aquatile and Fusobacterium necrogenes . The highest Hg accumulation from nutrient broth liquid media containing 10, 20 and 30 ppm Hg was observed for Brevundimonas vesicularis . The mercury accumulation efficiency of the four bacteria applied to small-scale gold mine tailing containing mercury was in the order of Fusobacterium aquatile (76.1%) > Brevundimonas vesicularis (75.6%) > Fusobacterium necrogenes (74.4%) > Nitrococcus mobilis (74.2%). On the basis of the Hg accumulation efficiency of more than 75%, Fusobacterium aquatile and Brevundimonas vesicularis are prospective for bioremediation of mercury-contaminated soils. |
topic |
mercury resistant bacteria small-scale gold mine tailing |
url |
http://www.journalssystem.com/jeeng/The-potential-of-mercury-resistant-bacteria-isolated-from-small-scale-gold-mine-tailings,83565,0,2.html |
work_keys_str_mv |
AT umichasanah thepotentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury AT yulianuraini thepotentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury AT ekohandayanto thepotentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury AT umichasanah potentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury AT yulianuraini potentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury AT ekohandayanto potentialofmercuryresistantbacteriaisolatedfromsmallscalegoldminetailingsforaccumulationofmercury |
_version_ |
1725808842529308672 |