Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution
Specific and quantitative sequencing of cytosine modifications is challenging at base-resolution. Here the authors present TAPSβ and CAPS for subtraction-free whole genome sequencing of 5mC and 5hmC.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-20920-2 |
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doaj-bff59ff2b80449c5bd60715c079a9f1f |
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Article |
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doaj-bff59ff2b80449c5bd60715c079a9f1f2021-01-31T12:19:48ZengNature Publishing GroupNature Communications2041-17232021-01-011211810.1038/s41467-021-20920-2Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolutionYibin Liu0Zhiyuan Hu1Jingfei Cheng2Paulina Siejka-Zielińska3Jinfeng Chen4Masato Inoue5Ahmed Ashour Ahmed6Chun-Xiao Song7Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordOvarian Cancer Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of OxfordLudwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordLudwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordLudwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordLudwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordOvarian Cancer Cell Laboratory, MRC Weatherall Institute of Molecular Medicine, University of OxfordLudwig Institute for Cancer Research, Nuffield Department of Medicine, University of OxfordSpecific and quantitative sequencing of cytosine modifications is challenging at base-resolution. Here the authors present TAPSβ and CAPS for subtraction-free whole genome sequencing of 5mC and 5hmC.https://doi.org/10.1038/s41467-021-20920-2 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yibin Liu Zhiyuan Hu Jingfei Cheng Paulina Siejka-Zielińska Jinfeng Chen Masato Inoue Ahmed Ashour Ahmed Chun-Xiao Song |
| spellingShingle |
Yibin Liu Zhiyuan Hu Jingfei Cheng Paulina Siejka-Zielińska Jinfeng Chen Masato Inoue Ahmed Ashour Ahmed Chun-Xiao Song Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution Nature Communications |
| author_facet |
Yibin Liu Zhiyuan Hu Jingfei Cheng Paulina Siejka-Zielińska Jinfeng Chen Masato Inoue Ahmed Ashour Ahmed Chun-Xiao Song |
| author_sort |
Yibin Liu |
| title |
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| title_short |
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| title_full |
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| title_fullStr |
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| title_full_unstemmed |
Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| title_sort |
subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2021-01-01 |
| description |
Specific and quantitative sequencing of cytosine modifications is challenging at base-resolution. Here the authors present TAPSβ and CAPS for subtraction-free whole genome sequencing of 5mC and 5hmC. |
| url |
https://doi.org/10.1038/s41467-021-20920-2 |
| work_keys_str_mv |
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