Characterization of Chlorella sorokiniana, UTEX 1230
This paper characterizes the strain Chlorella sorokiniana UTEX 1230 within a laboratory setting using a 1 L bubble column. The findings show that productivity can be trebled under mixotrophic conditions (from 0.2 g·L−1·d−1 to 0.66 g·L−1&...
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doaj-c01b754ab7f24f6aa1b0e0554b775d3b2020-11-25T01:01:06ZengMDPI AGBiology2079-77372018-04-01722510.3390/biology7020025biology7020025Characterization of Chlorella sorokiniana, UTEX 1230Alessandro Marco Lizzul0Aitor Lekuona-Amundarain1Saul Purton2Luiza Cintra Campos3Unit 12, Ball Mill Top Business Park, Hallow, Worcester WR2 6PD, UKDepartment of Environmental Engineering, University College London, Gower Street, London WC1E 6BT, UKDepartment of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UKDepartment of Environmental Engineering, University College London, Gower Street, London WC1E 6BT, UKThis paper characterizes the strain Chlorella sorokiniana UTEX 1230 within a laboratory setting using a 1 L bubble column. The findings show that productivity can be trebled under mixotrophic conditions (from 0.2 g·L−1·d−1 to 0.66 g·L−1·d−1) with the addition of sodium acetate. The results also indicate that both the growth rate and final yield increase with the cultivation temperature, with most parameters showing an optimum in the range of 30–35 °C. The maximum specific growth rate was found to be in the region of 0.12 h−1 at a surface irradiance between 100–500 µE·m−2·s−1. This high growth rate makes the strain particularly suited to the rapid production of biomass, suitable for either whole cell bioprocessing or bioremediation. However, the relatively low lipid productivity (9.2 mg·L−1·d−1) confirms previous findings which would indicate poor applicability for biodiesel production. The strain shows greater promise in wastewater treatment applications with removal rates of nitrogen and phosphorus in the region of 37 and 30 mg·L−1·d−1 respectively. Furthermore, the findings show that a fed-batch strategy to inorganic nutrient loading can increase the final yield by around 50% compared to a conventional batch run. This is particularly interesting as fed-batch production techniques are rarely used within microalgal cultivation, so provide an interesting avenue for further investigation. Overall, the findings show that C. sorokiniana UTEX 1230 is a robust and fast-growing microalgal strain suitable both for the laboratory and scale-up.http://www.mdpi.com/2079-7737/7/2/25Chlorella sorokinianaUTEX1230productivityyieldcharacterization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alessandro Marco Lizzul Aitor Lekuona-Amundarain Saul Purton Luiza Cintra Campos |
spellingShingle |
Alessandro Marco Lizzul Aitor Lekuona-Amundarain Saul Purton Luiza Cintra Campos Characterization of Chlorella sorokiniana, UTEX 1230 Biology Chlorella sorokiniana UTEX1230 productivity yield characterization |
author_facet |
Alessandro Marco Lizzul Aitor Lekuona-Amundarain Saul Purton Luiza Cintra Campos |
author_sort |
Alessandro Marco Lizzul |
title |
Characterization of Chlorella sorokiniana, UTEX 1230 |
title_short |
Characterization of Chlorella sorokiniana, UTEX 1230 |
title_full |
Characterization of Chlorella sorokiniana, UTEX 1230 |
title_fullStr |
Characterization of Chlorella sorokiniana, UTEX 1230 |
title_full_unstemmed |
Characterization of Chlorella sorokiniana, UTEX 1230 |
title_sort |
characterization of chlorella sorokiniana, utex 1230 |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2018-04-01 |
description |
This paper characterizes the strain Chlorella sorokiniana UTEX 1230 within a laboratory setting using a 1 L bubble column. The findings show that productivity can be trebled under mixotrophic conditions (from 0.2 g·L−1·d−1 to 0.66 g·L−1·d−1) with the addition of sodium acetate. The results also indicate that both the growth rate and final yield increase with the cultivation temperature, with most parameters showing an optimum in the range of 30–35 °C. The maximum specific growth rate was found to be in the region of 0.12 h−1 at a surface irradiance between 100–500 µE·m−2·s−1. This high growth rate makes the strain particularly suited to the rapid production of biomass, suitable for either whole cell bioprocessing or bioremediation. However, the relatively low lipid productivity (9.2 mg·L−1·d−1) confirms previous findings which would indicate poor applicability for biodiesel production. The strain shows greater promise in wastewater treatment applications with removal rates of nitrogen and phosphorus in the region of 37 and 30 mg·L−1·d−1 respectively. Furthermore, the findings show that a fed-batch strategy to inorganic nutrient loading can increase the final yield by around 50% compared to a conventional batch run. This is particularly interesting as fed-batch production techniques are rarely used within microalgal cultivation, so provide an interesting avenue for further investigation. Overall, the findings show that C. sorokiniana UTEX 1230 is a robust and fast-growing microalgal strain suitable both for the laboratory and scale-up. |
topic |
Chlorella sorokiniana UTEX1230 productivity yield characterization |
url |
http://www.mdpi.com/2079-7737/7/2/25 |
work_keys_str_mv |
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