The correlation of p16 and MMPs expression in cervical secretions with the angiogenesis and cell proliferation in cervical cancer tissue

• Main Author: Lai-Fang Zhu1,2
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2017-04-01
• Series: Journal of Hainan Medical University
• Subjects: Cervical cancer p16 Matrix metalloproteinase Angiogenesis Cell proliferation
• Online Access: http://www.hnykdxxb.com/PDF/201708/31.pdf

Objective: To study the correlation of p16 and MMPs expression in cervical secretions with the angiogenesis and cell proliferation in cervical cancer tissue. Methods: A total of 78 patients with cervical cancer who were treated in our hospital between August 2013 and May 2016 were collected as the observation group, and patients with cervicitis who received examination in our hospital during the same period were selected as the control group. The cervical secretions were collected to determine p16, MMP-2, MMP-7 and MMP-9 levels with enzyme-linked immunosorbent assay (ELISA) kit, cervical lesion tissue samples were collected to determine angiogenesis index levels by RIA method, and ELISA kits were used to determine proliferation genes B7-H4, GBP1, Sp2, EZH2 and PKCε protein expression. Pearson test was used to analyze the correlation of p16 and MMPs expression in cervical secretions with the angiogenesis and cell proliferation in cervical cancer tissue of patients with cervical cancer. Results: p16, MMP-2, MMP-7 and MMP-9 levels in cervical secretions of observation group were higher than those of control group, and COX-2, VEGFA, VEGFC, B7-H4, Sp2, EZH2 and PKCε expression in cervical biopsy tissue were higher than those of control group while GBP1 expression was lower than that of control group. Conclusion: p16 and MMPs expression in cervical secretions of patients with cervical cancer can quantifiably reflect the degree of angiogenesis and proliferation of cervical cancer cells.