Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms
Purpose. Excessive glutamate amount can give oxidative stress to neuronal cells, and the accumulation of cell death can trigger the neurodegenerative disorders. In this study, we discovered the neuroprotective effect of Lysimachia christinae Hance in the mouse hippocampal HT22 cell line. Method. Ove...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2020/5352034 |
id |
doaj-c02c9b9c00a241a682006d571d9d1b33 |
---|---|
record_format |
Article |
spelling |
doaj-c02c9b9c00a241a682006d571d9d1b332020-11-25T02:06:37ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882020-01-01202010.1155/2020/53520345352034Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective MechanismsGahee Ryu0Choong Je Ma1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of KoreaDepartment of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of KoreaPurpose. Excessive glutamate amount can give oxidative stress to neuronal cells, and the accumulation of cell death can trigger the neurodegenerative disorders. In this study, we discovered the neuroprotective effect of Lysimachia christinae Hance in the mouse hippocampal HT22 cell line. Method. Overnight incubated HT22 cells were pretreated with L. christinae extract dose dependently (1, 10, and 100 μg/ml). Followed by then, glutamate was treated. These treated cells were incubated several times again, and cell viability, accumulation of reactive oxygen species (ROS) and Ca2+, mitochondrial membrane potential (MMP), and glutathione-related enzyme amount were measured. Results. As a result, L. christinae increases the cell viability by inhibiting the ROS and Ca2+ formation, recovering the level of MMP and enhancing the activity of glutathione production compared with only vehicle-treated groups. Conclusion. These draw that L. christinae may remarkably decelerate the neurodegeneration by minimizing neuronal cell damage via oxidative stress.http://dx.doi.org/10.1155/2020/5352034 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gahee Ryu Choong Je Ma |
spellingShingle |
Gahee Ryu Choong Je Ma Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms Evidence-Based Complementary and Alternative Medicine |
author_facet |
Gahee Ryu Choong Je Ma |
author_sort |
Gahee Ryu |
title |
Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms |
title_short |
Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms |
title_full |
Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms |
title_fullStr |
Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms |
title_full_unstemmed |
Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms |
title_sort |
neuroprotective activity of methanolic extract of lysimachia christinae against glutamate toxicity in ht22 cell and its protective mechanisms |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2020-01-01 |
description |
Purpose. Excessive glutamate amount can give oxidative stress to neuronal cells, and the accumulation of cell death can trigger the neurodegenerative disorders. In this study, we discovered the neuroprotective effect of Lysimachia christinae Hance in the mouse hippocampal HT22 cell line. Method. Overnight incubated HT22 cells were pretreated with L. christinae extract dose dependently (1, 10, and 100 μg/ml). Followed by then, glutamate was treated. These treated cells were incubated several times again, and cell viability, accumulation of reactive oxygen species (ROS) and Ca2+, mitochondrial membrane potential (MMP), and glutathione-related enzyme amount were measured. Results. As a result, L. christinae increases the cell viability by inhibiting the ROS and Ca2+ formation, recovering the level of MMP and enhancing the activity of glutathione production compared with only vehicle-treated groups. Conclusion. These draw that L. christinae may remarkably decelerate the neurodegeneration by minimizing neuronal cell damage via oxidative stress. |
url |
http://dx.doi.org/10.1155/2020/5352034 |
work_keys_str_mv |
AT gaheeryu neuroprotectiveactivityofmethanolicextractoflysimachiachristinaeagainstglutamatetoxicityinht22cellanditsprotectivemechanisms AT choongjema neuroprotectiveactivityofmethanolicextractoflysimachiachristinaeagainstglutamatetoxicityinht22cellanditsprotectivemechanisms |
_version_ |
1715569444531470336 |