Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation.
Bone marrow mesenchymal stem cells (BMMSCs) have shown immunosuppressive activity in transplantation. This study was designed to determine whether BMMSCs could improve outcomes of small bowel transplantation in rats.Heterotopic small bowel transplantation was performed from Brown Norway to Lewis rat...
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doaj-c03376c0928b424283db0796a08ef1d22020-11-25T02:45:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11452810.1371/journal.pone.0114528Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation.Yang YangHong-Li SongWen ZhangBen-Juan WuNan-Nan FuWei-Ping ZhengChong DongZhong-Yang ShenBone marrow mesenchymal stem cells (BMMSCs) have shown immunosuppressive activity in transplantation. This study was designed to determine whether BMMSCs could improve outcomes of small bowel transplantation in rats.Heterotopic small bowel transplantation was performed from Brown Norway to Lewis rats, followed by infusion of BMMSCs through the superficial dorsal veins of the penis. Controls included rats infused with normal saline (allogeneic control), isogeneically transplanted rats (BN-BN) and nontransplanted animals. The animals were sacrificed after 1, 5, 7 or 10 days. Small bowel histology and apoptosis, cytokine concentrations in serum and intestinal grafts, and numbers of T regulatory (Treg) cells were assessed at each time point.Acute cellular rejection occurred soon after transplantation and became aggravated over time in the allogeneic control rats, with increase in apoptosis, inflammatory response, and T helper (Th)1/Th2 and Th17/Treg-related cytokines. BMMSCs significantly attenuated acute cellular rejection, reduced apoptosis and suppressed the concentrations of interleukin (IL)-2, IL-6, IL-17, IL-23, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ while upregulating IL-10 and transforming growth factor (TGF)-β expression and increasing Treg levels.BMMSCs improve the outcomes of allogeneic small bowel transplantation by attenuating the inflammatory response and acute cellular rejection. Treatment with BMMSCs may overcome acute cellular rejection in small bowel transplantation.http://europepmc.org/articles/PMC4266507?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Yang Hong-Li Song Wen Zhang Ben-Juan Wu Nan-Nan Fu Wei-Ping Zheng Chong Dong Zhong-Yang Shen |
spellingShingle |
Yang Yang Hong-Li Song Wen Zhang Ben-Juan Wu Nan-Nan Fu Wei-Ping Zheng Chong Dong Zhong-Yang Shen Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. PLoS ONE |
author_facet |
Yang Yang Hong-Li Song Wen Zhang Ben-Juan Wu Nan-Nan Fu Wei-Ping Zheng Chong Dong Zhong-Yang Shen |
author_sort |
Yang Yang |
title |
Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
title_short |
Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
title_full |
Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
title_fullStr |
Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
title_full_unstemmed |
Reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
title_sort |
reduction of acute rejection by bone marrow mesenchymal stem cells during rat small bowel transplantation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Bone marrow mesenchymal stem cells (BMMSCs) have shown immunosuppressive activity in transplantation. This study was designed to determine whether BMMSCs could improve outcomes of small bowel transplantation in rats.Heterotopic small bowel transplantation was performed from Brown Norway to Lewis rats, followed by infusion of BMMSCs through the superficial dorsal veins of the penis. Controls included rats infused with normal saline (allogeneic control), isogeneically transplanted rats (BN-BN) and nontransplanted animals. The animals were sacrificed after 1, 5, 7 or 10 days. Small bowel histology and apoptosis, cytokine concentrations in serum and intestinal grafts, and numbers of T regulatory (Treg) cells were assessed at each time point.Acute cellular rejection occurred soon after transplantation and became aggravated over time in the allogeneic control rats, with increase in apoptosis, inflammatory response, and T helper (Th)1/Th2 and Th17/Treg-related cytokines. BMMSCs significantly attenuated acute cellular rejection, reduced apoptosis and suppressed the concentrations of interleukin (IL)-2, IL-6, IL-17, IL-23, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ while upregulating IL-10 and transforming growth factor (TGF)-β expression and increasing Treg levels.BMMSCs improve the outcomes of allogeneic small bowel transplantation by attenuating the inflammatory response and acute cellular rejection. Treatment with BMMSCs may overcome acute cellular rejection in small bowel transplantation. |
url |
http://europepmc.org/articles/PMC4266507?pdf=render |
work_keys_str_mv |
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