Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple
Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established: immersion system (2 L twin flasks temporary immers...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Brazilian Society of Plant Breeding
2015-04-01
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| Series: | Crop Breeding and Applied Biotechnology |
| Subjects: | |
| Online Access: | http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-e458-11df.pdf |
| Summary: | Micropropagation based techniques enable the mass production of pest- and disease-free stock plants. Aiming at optimizing
pineapple [Ananas comosus (L.) Merril] micropropagation, a complete randomized experiment with three factors was established:
immersion system (2 L twin flasks temporary immersion and permanent immersion); culture medium (supplemented or not with GA3
);
and shoot height class. The effects on regeneration rate (number of new shoots per 1 g fresh mass (FM) of inoculated microshoots),
FM per shoot, and shoot survival were evaluated. Significant interactions (p<0.05) were observed in the regeneration rate, and simple
effects (p<0.05) were detected for FM per shoot. All treatments resulted in a survival rate higher than 95%. Altogether, it was found
that 2 L twin flasks temporary immersion and MS culture medium free of GA3
allowed the optimization of the protocol used. This
treatment was tested with positive response in a biofactory system that uses sterile plastic bottles (5 L).
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| ISSN: | 1518-7853 1984-7033 |