Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve

Summary: Single-molecule fluorescence in situ hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target sp...

Full description

Bibliographic Details
Main Authors: Xinbei Li, Sreenivas Eadara, Sangmin Jeon, Yan Liu, Gabriella Muwanga, Lintao Qu, Michael J. Caterina, Mollie K. Meffert
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Microscopy
Molecular Biology
In Situ Hybridization
Neuroscience
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002628

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721002628

Similar Items