Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis
Pythiosis, a life-threatening disease caused by <i>Pythium insidiosum</i>, has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected...
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doaj-c053fee07b7e46459833ebd338216f5a2021-06-30T23:58:56ZengMDPI AGJournal of Fungi2309-608X2021-06-01747447410.3390/jof7060474Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for PythiosisTiwa Rotchanapreeda0Pattarana Sae-Chew1Tassanee Lohnoo2Wanta Yingyong3Thidarat Rujirawat4Yothin Kumsang5Penpan Payattikul6Chalisa Jaturapaktrarak7Akarin Intaramat8Watcharapol Pathomsakulwong9Chompoonek Yurayart10Theerapong Krajaejun11Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandTranslational Research Unit and Laboratory of Immunology, Chulabhorn Research Institute, Bangkok 10210, ThailandEquine Clinic, Kasetsart University Veterinary Teaching Hospital, Nakhon Pathom 73140, ThailandDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, ThailandDepartment of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandPythiosis, a life-threatening disease caused by <i>Pythium insidiosum</i>, has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected the laboratory reports of 38 animals with suspected pythiosis and detected ICT false-positive results in three horses and a dog with basidiobolomycosis. <i>P. insidiosum</i> and <i>Basidiobolus ranarum</i> cause infections with indistinguishable clinical and microscopic features. This study investigated cross-reactive antibodies by probing <i>P. insidiosum</i> and <i>B. ranarum</i> crude extracts and cell-free synthesized I06 protein (encoded in <i>P. insidiosum</i> genome, not other fungi) against a panel of pythiosis, basidiobolomycosis, rabbit anti-I06 peptide, and control sera by Western blot analyses. ICT false-positive results occurred from the cross-reactivity of anti-<i>B. ranarum</i> antibodies to the 15, 50, 60, and 120 kDa proteins of <i>P. insidiosum</i>, not double infections caused by both pathogens. Notably, ICT could help to screen pythiosis, and the positive test requires confirmation by culture or molecular method. The detection specificity of ICT requires improvement. The crude extract containing multispecies antigens needs replacement with a refined <i>P. insidiosum</i>-specific protein. We proposed that the 55 kDa I06 protein is an excellent candidate for developing a more specific serodiagnostic test for pythiosis.https://www.mdpi.com/2309-608X/7/6/474pythiosis<i>Pythium insidiosum</i><i>Basidiobolus ranarum</i>cross-reactivityserodiagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tiwa Rotchanapreeda Pattarana Sae-Chew Tassanee Lohnoo Wanta Yingyong Thidarat Rujirawat Yothin Kumsang Penpan Payattikul Chalisa Jaturapaktrarak Akarin Intaramat Watcharapol Pathomsakulwong Chompoonek Yurayart Theerapong Krajaejun |
spellingShingle |
Tiwa Rotchanapreeda Pattarana Sae-Chew Tassanee Lohnoo Wanta Yingyong Thidarat Rujirawat Yothin Kumsang Penpan Payattikul Chalisa Jaturapaktrarak Akarin Intaramat Watcharapol Pathomsakulwong Chompoonek Yurayart Theerapong Krajaejun Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis Journal of Fungi pythiosis <i>Pythium insidiosum</i> <i>Basidiobolus ranarum</i> cross-reactivity serodiagnosis |
author_facet |
Tiwa Rotchanapreeda Pattarana Sae-Chew Tassanee Lohnoo Wanta Yingyong Thidarat Rujirawat Yothin Kumsang Penpan Payattikul Chalisa Jaturapaktrarak Akarin Intaramat Watcharapol Pathomsakulwong Chompoonek Yurayart Theerapong Krajaejun |
author_sort |
Tiwa Rotchanapreeda |
title |
Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis |
title_short |
Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis |
title_full |
Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis |
title_fullStr |
Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis |
title_full_unstemmed |
Immunological Cross-Reactivity of Proteins Extracted from the Oomycete <i>Pythium insidiosum</i> and the Fungus <i>Basidiobolus ranarum</i> Compromises the Detection Specificity of Immunodiagnostic Assays for Pythiosis |
title_sort |
immunological cross-reactivity of proteins extracted from the oomycete <i>pythium insidiosum</i> and the fungus <i>basidiobolus ranarum</i> compromises the detection specificity of immunodiagnostic assays for pythiosis |
publisher |
MDPI AG |
series |
Journal of Fungi |
issn |
2309-608X |
publishDate |
2021-06-01 |
description |
Pythiosis, a life-threatening disease caused by <i>Pythium insidiosum</i>, has been increasingly diagnosed worldwide. A recently developed immunochromatographic test (ICT) enables the rapid diagnosis of pythiosis. During the 3-year clinical implementation of ICT in Thailand, we collected the laboratory reports of 38 animals with suspected pythiosis and detected ICT false-positive results in three horses and a dog with basidiobolomycosis. <i>P. insidiosum</i> and <i>Basidiobolus ranarum</i> cause infections with indistinguishable clinical and microscopic features. This study investigated cross-reactive antibodies by probing <i>P. insidiosum</i> and <i>B. ranarum</i> crude extracts and cell-free synthesized I06 protein (encoded in <i>P. insidiosum</i> genome, not other fungi) against a panel of pythiosis, basidiobolomycosis, rabbit anti-I06 peptide, and control sera by Western blot analyses. ICT false-positive results occurred from the cross-reactivity of anti-<i>B. ranarum</i> antibodies to the 15, 50, 60, and 120 kDa proteins of <i>P. insidiosum</i>, not double infections caused by both pathogens. Notably, ICT could help to screen pythiosis, and the positive test requires confirmation by culture or molecular method. The detection specificity of ICT requires improvement. The crude extract containing multispecies antigens needs replacement with a refined <i>P. insidiosum</i>-specific protein. We proposed that the 55 kDa I06 protein is an excellent candidate for developing a more specific serodiagnostic test for pythiosis. |
topic |
pythiosis <i>Pythium insidiosum</i> <i>Basidiobolus ranarum</i> cross-reactivity serodiagnosis |
url |
https://www.mdpi.com/2309-608X/7/6/474 |
work_keys_str_mv |
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