Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System
The production of functional islet-like cells from human-induced pluripotent stem cells (hiPSCs) is a promising strategy for the therapeutic use and disease modeling for type 1 diabetes. However, the production cost of islet-like cells is extremely high due to the use of expensive growth factors for...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-08-01
|
Series: | Cells |
Subjects: | |
Online Access: | https://www.mdpi.com/2073-4409/10/8/2017 |
id |
doaj-c05a68af74544e2fb6acc0ae036ada61 |
---|---|
record_format |
Article |
spelling |
doaj-c05a68af74544e2fb6acc0ae036ada612021-08-26T13:37:24ZengMDPI AGCells2073-44092021-08-01102017201710.3390/cells10082017Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture SystemHyunjin Choi0Marie Shinohara1Masato Ibuki2Masaki Nishikawa3Yasuyuki Sakai4Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, JapanInstitute of Industrial Science, The University of Tokyo, Tokyo 153-8505, JapanInstitute of Industrial Science, The University of Tokyo, Tokyo 153-8505, JapanDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, JapanDepartment of Chemical System Engineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8654, JapanThe production of functional islet-like cells from human-induced pluripotent stem cells (hiPSCs) is a promising strategy for the therapeutic use and disease modeling for type 1 diabetes. However, the production cost of islet-like cells is extremely high due to the use of expensive growth factors for differentiation. In a conventional culture method, growth factors and beneficial autocrine factors remaining in the culture medium are removed along with toxic metabolites during the medium change, and it limits the efficient utilization of those factors. In this study, we demonstrated that the dialysis suspension culture system is possible to reduce the usage of growth factors to one-third in the differentiation of hiPSC-derived endocrine progenitor cells to islet-like cells by reducing the medium change frequency with the refinement of the culture medium. Furthermore, the expression levels of hormone-secretion-related genes and the efficiency of differentiation were improved with the dialysis suspension culture system, possibly due to the retaining of autocrine factors. In addition, we confirmed several improvements required for the further study of the dialysis culture system. These findings showed the promising possibility of the dialysis suspension culture system for the low-cost production of islet-like cells.https://www.mdpi.com/2073-4409/10/8/2017pancreatic β cellislet-like cellhiPSCdifferentiationdialysissuspension culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hyunjin Choi Marie Shinohara Masato Ibuki Masaki Nishikawa Yasuyuki Sakai |
spellingShingle |
Hyunjin Choi Marie Shinohara Masato Ibuki Masaki Nishikawa Yasuyuki Sakai Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System Cells pancreatic β cell islet-like cell hiPSC differentiation dialysis suspension culture |
author_facet |
Hyunjin Choi Marie Shinohara Masato Ibuki Masaki Nishikawa Yasuyuki Sakai |
author_sort |
Hyunjin Choi |
title |
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System |
title_short |
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System |
title_full |
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System |
title_fullStr |
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System |
title_full_unstemmed |
Differentiation of Human-Induced Pluripotent Stem Cell-Derived Endocrine Progenitors to Islet-like Cells Using a Dialysis Suspension Culture System |
title_sort |
differentiation of human-induced pluripotent stem cell-derived endocrine progenitors to islet-like cells using a dialysis suspension culture system |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-08-01 |
description |
The production of functional islet-like cells from human-induced pluripotent stem cells (hiPSCs) is a promising strategy for the therapeutic use and disease modeling for type 1 diabetes. However, the production cost of islet-like cells is extremely high due to the use of expensive growth factors for differentiation. In a conventional culture method, growth factors and beneficial autocrine factors remaining in the culture medium are removed along with toxic metabolites during the medium change, and it limits the efficient utilization of those factors. In this study, we demonstrated that the dialysis suspension culture system is possible to reduce the usage of growth factors to one-third in the differentiation of hiPSC-derived endocrine progenitor cells to islet-like cells by reducing the medium change frequency with the refinement of the culture medium. Furthermore, the expression levels of hormone-secretion-related genes and the efficiency of differentiation were improved with the dialysis suspension culture system, possibly due to the retaining of autocrine factors. In addition, we confirmed several improvements required for the further study of the dialysis culture system. These findings showed the promising possibility of the dialysis suspension culture system for the low-cost production of islet-like cells. |
topic |
pancreatic β cell islet-like cell hiPSC differentiation dialysis suspension culture |
url |
https://www.mdpi.com/2073-4409/10/8/2017 |
work_keys_str_mv |
AT hyunjinchoi differentiationofhumaninducedpluripotentstemcellderivedendocrineprogenitorstoisletlikecellsusingadialysissuspensionculturesystem AT marieshinohara differentiationofhumaninducedpluripotentstemcellderivedendocrineprogenitorstoisletlikecellsusingadialysissuspensionculturesystem AT masatoibuki differentiationofhumaninducedpluripotentstemcellderivedendocrineprogenitorstoisletlikecellsusingadialysissuspensionculturesystem AT masakinishikawa differentiationofhumaninducedpluripotentstemcellderivedendocrineprogenitorstoisletlikecellsusingadialysissuspensionculturesystem AT yasuyukisakai differentiationofhumaninducedpluripotentstemcellderivedendocrineprogenitorstoisletlikecellsusingadialysissuspensionculturesystem |
_version_ |
1721194404598775808 |