Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice
Abstract Ovol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Here, we firstly examined the mRNA and protein levels of Ovol2 in devel...
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doaj-c05fdc561b044cf1a59dfb771849d61c2020-11-25T04:03:17ZengBMCReproductive Biology and Endocrinology1477-78272019-11-011711410.1186/s12958-019-0542-3Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in miceJin Zhang0Juan Dong1Weibing Qin2Congcong Cao3Yujiao Wen4Yunge Tang5Shuiqiao Yuan6Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyNHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong ProvinceInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyNHC Key Laboratory of Male Reproduction and Genetics, Family Planning Research Institute of Guangdong ProvinceInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Ovol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Here, we firstly examined the mRNA and protein levels of Ovol2 in developing mouse testes by RT-qPCR and western blot and found that both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its higher level at adult testis. Further testicular immuno-staining revealed that OVOL2 is highly expressed in the spermatogonia, spermatocytes and round spermatids. Interestingly, our conditional ovol2 knockout mouse model show that loss of ovol2 in embryonic germ cells does not affect fecundity in mice. Our data also show that Ovol1 may have compensated for the loss of Ovol2 functions in germ cells. Overall, our data indicate that ovol2 is dispensable for germ cell development and spermatogenesis.http://link.springer.com/article/10.1186/s12958-019-0542-3Ovol2SpermatogenesisFertilityKnockout mice |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Zhang Juan Dong Weibing Qin Congcong Cao Yujiao Wen Yunge Tang Shuiqiao Yuan |
spellingShingle |
Jin Zhang Juan Dong Weibing Qin Congcong Cao Yujiao Wen Yunge Tang Shuiqiao Yuan Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice Reproductive Biology and Endocrinology Ovol2 Spermatogenesis Fertility Knockout mice |
author_facet |
Jin Zhang Juan Dong Weibing Qin Congcong Cao Yujiao Wen Yunge Tang Shuiqiao Yuan |
author_sort |
Jin Zhang |
title |
Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
title_short |
Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
title_full |
Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
title_fullStr |
Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
title_full_unstemmed |
Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
title_sort |
ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2019-11-01 |
description |
Abstract Ovol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Here, we firstly examined the mRNA and protein levels of Ovol2 in developing mouse testes by RT-qPCR and western blot and found that both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its higher level at adult testis. Further testicular immuno-staining revealed that OVOL2 is highly expressed in the spermatogonia, spermatocytes and round spermatids. Interestingly, our conditional ovol2 knockout mouse model show that loss of ovol2 in embryonic germ cells does not affect fecundity in mice. Our data also show that Ovol1 may have compensated for the loss of Ovol2 functions in germ cells. Overall, our data indicate that ovol2 is dispensable for germ cell development and spermatogenesis. |
topic |
Ovol2 Spermatogenesis Fertility Knockout mice |
url |
http://link.springer.com/article/10.1186/s12958-019-0542-3 |
work_keys_str_mv |
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