A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.)
Abstract Background Brassica napus L. has little or no primary dormancy, but exhibits great variation in secondary dormancy. Secondary dormancy potential in oilseed rape can lead to the emergence of volunteer plants that cause genetic contamination, reduced quality and biosafety issues. However, the...
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doaj-c06c3cbf6cbd4bdc864e3d1afed756a52020-11-25T03:47:17ZengBMCBMC Plant Biology1471-22292019-06-0119111810.1186/s12870-019-1866-zA transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.)Lei Liu0Fuxia Liu1Jinfang Chu2Xin Yi3Wenqi Fan4Tang Tang5Guimin Chen6Qiuhuan Guo7Xiangxiang Zhao8Jiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityNational Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of SciencesJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityNational Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of SciencesJiangsu Key Laboratory for Eco-agriculture Biotechnology around Hongze Lake, Huaiyin Normal UniversityAbstract Background Brassica napus L. has little or no primary dormancy, but exhibits great variation in secondary dormancy. Secondary dormancy potential in oilseed rape can lead to the emergence of volunteer plants that cause genetic contamination, reduced quality and biosafety issues. However, the mechanisms underlying secondary dormancy are poorly understood. In this study, cultivars Huaiyou-WSD-H2 (H) and Huaiyou-SSD-V1 (V), which exhibit low (approximately 5%) and high (approximately 95%) secondary dormancy rate, respectively, were identified. Four samples, before (Hb and Vb) and after (Ha and Va) secondary dormancy induction by polyethylene glycol (PEG), were collected to identify the candidate genes involved in secondary dormancy via comparative transcriptome profile analysis. Results A total of 998 differentially expressed genes (DEGs), which are mainly involved in secondary metabolism, transcriptional regulation, protein modification and signaling pathways, were then detected. Among these DEGs, the expression levels of those involved in the sulfur-rich indole glucosinolate (GLS)-linked auxin biosynthesis pathway were markedly upregulated in the dormant seeds (Va), which were validated by qRT-PCR and subsequently confirmed via detection of altered concentrations of indole-3-acetic acid (IAA), IAA conjugates and precursors. Furthermore, exogenous IAA applications to cultivar H enhanced secondary dormancy. Conclusion This study first (to our knowledge) elucidated that indole GLS-linked auxin biosynthesis is enhanced during secondary dormancy induced by PEG, which provides valuable information concerning secondary dormancy and expands the current understanding of the role of auxin in rapeseed.http://link.springer.com/article/10.1186/s12870-019-1866-zBrassica napusSecondary dormancyGerminationVolunteer plantPhytohormoneRNA-seq analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Liu Fuxia Liu Jinfang Chu Xin Yi Wenqi Fan Tang Tang Guimin Chen Qiuhuan Guo Xiangxiang Zhao |
spellingShingle |
Lei Liu Fuxia Liu Jinfang Chu Xin Yi Wenqi Fan Tang Tang Guimin Chen Qiuhuan Guo Xiangxiang Zhao A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) BMC Plant Biology Brassica napus Secondary dormancy Germination Volunteer plant Phytohormone RNA-seq analysis |
author_facet |
Lei Liu Fuxia Liu Jinfang Chu Xin Yi Wenqi Fan Tang Tang Guimin Chen Qiuhuan Guo Xiangxiang Zhao |
author_sort |
Lei Liu |
title |
A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) |
title_short |
A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) |
title_full |
A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) |
title_fullStr |
A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) |
title_full_unstemmed |
A transcriptome analysis reveals a role for the indole GLS-linked auxin biosynthesis in secondary dormancy in rapeseed (Brassica napus L.) |
title_sort |
transcriptome analysis reveals a role for the indole gls-linked auxin biosynthesis in secondary dormancy in rapeseed (brassica napus l.) |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2019-06-01 |
description |
Abstract Background Brassica napus L. has little or no primary dormancy, but exhibits great variation in secondary dormancy. Secondary dormancy potential in oilseed rape can lead to the emergence of volunteer plants that cause genetic contamination, reduced quality and biosafety issues. However, the mechanisms underlying secondary dormancy are poorly understood. In this study, cultivars Huaiyou-WSD-H2 (H) and Huaiyou-SSD-V1 (V), which exhibit low (approximately 5%) and high (approximately 95%) secondary dormancy rate, respectively, were identified. Four samples, before (Hb and Vb) and after (Ha and Va) secondary dormancy induction by polyethylene glycol (PEG), were collected to identify the candidate genes involved in secondary dormancy via comparative transcriptome profile analysis. Results A total of 998 differentially expressed genes (DEGs), which are mainly involved in secondary metabolism, transcriptional regulation, protein modification and signaling pathways, were then detected. Among these DEGs, the expression levels of those involved in the sulfur-rich indole glucosinolate (GLS)-linked auxin biosynthesis pathway were markedly upregulated in the dormant seeds (Va), which were validated by qRT-PCR and subsequently confirmed via detection of altered concentrations of indole-3-acetic acid (IAA), IAA conjugates and precursors. Furthermore, exogenous IAA applications to cultivar H enhanced secondary dormancy. Conclusion This study first (to our knowledge) elucidated that indole GLS-linked auxin biosynthesis is enhanced during secondary dormancy induced by PEG, which provides valuable information concerning secondary dormancy and expands the current understanding of the role of auxin in rapeseed. |
topic |
Brassica napus Secondary dormancy Germination Volunteer plant Phytohormone RNA-seq analysis |
url |
http://link.springer.com/article/10.1186/s12870-019-1866-z |
work_keys_str_mv |
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