Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line
Ferroptosis, a form of iron‐dependent cell death caused by lipid peroxidation, has been implicated in neurological and other disorders. However, the mechanism of ferroptosis in oligodendrocytes is unclear. We tested the susceptibility of MO3.13 cells, an oligodendrocyte line, to ferroptosis after er...
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Online Access: | https://doi.org/10.1002/2211-5463.12923 |
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doaj-c071cc546b414b289b54f1b4ee2a63db2020-11-25T02:06:06ZengWileyFEBS Open Bio2211-54632020-09-011091758176410.1002/2211-5463.12923Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell lineTomonori Hoshino0Hodaka Yamakado1Ryosuke Takahashi2Shu‐ichi Matsuzawa3Department of Neurology Graduate School of Medicine Kyoto University Kyoto JapanDepartment of Neurology Graduate School of Medicine Kyoto University Kyoto JapanDepartment of Neurology Graduate School of Medicine Kyoto University Kyoto JapanDepartment of Neurology Graduate School of Medicine Kyoto University Kyoto JapanFerroptosis, a form of iron‐dependent cell death caused by lipid peroxidation, has been implicated in neurological and other disorders. However, the mechanism of ferroptosis in oligodendrocytes is unclear. We tested the susceptibility of MO3.13 cells, an oligodendrocyte line, to ferroptosis after erastin treatment. Immature MO3.13 cells were more susceptible to erastin‐induced ferroptosis than chemically differentiated mature MO3.13 cells. Increased expression of solute carrier family 7 member 11 (SLC7A11), which encodes a cystine/glutamate transporter, and greater glutathione concentrations were observed in mature compared with immature MO3.13 cells, linking glutathione to the resistance of mature MO3.13 cells to erastin‐induced ferroptosis. These findings highlight the usefulness of immature MO3.13 cells in studies of ferroptosis and investigations into neuropathologies that involve oligodendrocytes.https://doi.org/10.1002/2211-5463.12923cell deathferroptosisglutathioneMO3.13 cell lineoligodendrocyte |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomonori Hoshino Hodaka Yamakado Ryosuke Takahashi Shu‐ichi Matsuzawa |
spellingShingle |
Tomonori Hoshino Hodaka Yamakado Ryosuke Takahashi Shu‐ichi Matsuzawa Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line FEBS Open Bio cell death ferroptosis glutathione MO3.13 cell line oligodendrocyte |
author_facet |
Tomonori Hoshino Hodaka Yamakado Ryosuke Takahashi Shu‐ichi Matsuzawa |
author_sort |
Tomonori Hoshino |
title |
Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
title_short |
Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
title_full |
Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
title_fullStr |
Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
title_full_unstemmed |
Susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
title_sort |
susceptibility to erastin‐induced ferroptosis decreases during maturation in a human oligodendrocyte cell line |
publisher |
Wiley |
series |
FEBS Open Bio |
issn |
2211-5463 |
publishDate |
2020-09-01 |
description |
Ferroptosis, a form of iron‐dependent cell death caused by lipid peroxidation, has been implicated in neurological and other disorders. However, the mechanism of ferroptosis in oligodendrocytes is unclear. We tested the susceptibility of MO3.13 cells, an oligodendrocyte line, to ferroptosis after erastin treatment. Immature MO3.13 cells were more susceptible to erastin‐induced ferroptosis than chemically differentiated mature MO3.13 cells. Increased expression of solute carrier family 7 member 11 (SLC7A11), which encodes a cystine/glutamate transporter, and greater glutathione concentrations were observed in mature compared with immature MO3.13 cells, linking glutathione to the resistance of mature MO3.13 cells to erastin‐induced ferroptosis. These findings highlight the usefulness of immature MO3.13 cells in studies of ferroptosis and investigations into neuropathologies that involve oligodendrocytes. |
topic |
cell death ferroptosis glutathione MO3.13 cell line oligodendrocyte |
url |
https://doi.org/10.1002/2211-5463.12923 |
work_keys_str_mv |
AT tomonorihoshino susceptibilitytoerastininducedferroptosisdecreasesduringmaturationinahumanoligodendrocytecellline AT hodakayamakado susceptibilitytoerastininducedferroptosisdecreasesduringmaturationinahumanoligodendrocytecellline AT ryosuketakahashi susceptibilitytoerastininducedferroptosisdecreasesduringmaturationinahumanoligodendrocytecellline AT shuichimatsuzawa susceptibilitytoerastininducedferroptosisdecreasesduringmaturationinahumanoligodendrocytecellline |
_version_ |
1724935012557520896 |