Association of Salmonella virulence factor alleles with intestinal and invasive serovars
Abstract Background The role of Salmonella virulence factor (VF) allelic variation in modulating pathogenesis or host specificity has only been demonstrated in a few cases, mostly through serendipitous findings. Virulence factor (VF) alleles from Salmonella enterica subsp. enterica genomes were comp...
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doaj-c08052b19db345f0a54d1b41228843b12020-11-25T03:18:09ZengBMCBMC Genomics1471-21642019-05-0120111410.1186/s12864-019-5809-8Association of Salmonella virulence factor alleles with intestinal and invasive serovarsAlexey V. Rakov0Emilio Mastriani1Shu-Lin Liu2Dieter M. Schifferli3Department of Pathobiology, School of Veterinary Medicine, University of PennsylvaniaSystemomics Center, College of Pharmacy, Genomics Research Center, State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Harbin Medical UniversitySystemomics Center, College of Pharmacy, Genomics Research Center, State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Harbin Medical UniversityDepartment of Pathobiology, School of Veterinary Medicine, University of PennsylvaniaAbstract Background The role of Salmonella virulence factor (VF) allelic variation in modulating pathogenesis or host specificity has only been demonstrated in a few cases, mostly through serendipitous findings. Virulence factor (VF) alleles from Salmonella enterica subsp. enterica genomes were compared to identify potential associations with the host-adapted invasive serovars Typhi, Dublin, Choleraesuis, and Gallinarum, and with the broad host-range intestinal serovars Typhimurium, Enteritidis, and Newport. Results Through a bioinformatics analysis of 500 Salmonella genomes, we have identified allelic variants of 70 VFs, many of which are associated with either one of the four host-adapted invasive Salmonella serovars or one of the three broad host-range intestinal serovars. In addition, associations between specific VF alleles and intra-serovar clusters, sequence types (STs) and/or host-adapted FimH adhesins were identified. Moreover, new allelic VF associations with non-typhoidal S. Enteritidis and S. Typhimurium (NTS) or invasive NTS (iNTS) were detected. Conclusions By analogy to the previously shown association of specific FimH adhesin alleles with optimal binding by host adapted Salmonella serovars, lineages or strains, we predict that some of the identified association of other VF alleles with host-adapted serovars, lineages or strains will reflect specific contributions to host adaptation and/or pathogenesis. The identification of these allelic associations will support investigations of the biological impact of VF alleles and better characterize the role of allelic variation in Salmonella pathogenesis. Most relevant functional experiments will test the potential causal contribution of the detected FimH-associated VF variants in host adapted virulence.http://link.springer.com/article/10.1186/s12864-019-5809-8Salmonella entericavirulence factorsallelic variationhost adaptationFimHSTs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexey V. Rakov Emilio Mastriani Shu-Lin Liu Dieter M. Schifferli |
spellingShingle |
Alexey V. Rakov Emilio Mastriani Shu-Lin Liu Dieter M. Schifferli Association of Salmonella virulence factor alleles with intestinal and invasive serovars BMC Genomics Salmonella enterica virulence factors allelic variation host adaptation FimH STs |
author_facet |
Alexey V. Rakov Emilio Mastriani Shu-Lin Liu Dieter M. Schifferli |
author_sort |
Alexey V. Rakov |
title |
Association of Salmonella virulence factor alleles with intestinal and invasive serovars |
title_short |
Association of Salmonella virulence factor alleles with intestinal and invasive serovars |
title_full |
Association of Salmonella virulence factor alleles with intestinal and invasive serovars |
title_fullStr |
Association of Salmonella virulence factor alleles with intestinal and invasive serovars |
title_full_unstemmed |
Association of Salmonella virulence factor alleles with intestinal and invasive serovars |
title_sort |
association of salmonella virulence factor alleles with intestinal and invasive serovars |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2019-05-01 |
description |
Abstract Background The role of Salmonella virulence factor (VF) allelic variation in modulating pathogenesis or host specificity has only been demonstrated in a few cases, mostly through serendipitous findings. Virulence factor (VF) alleles from Salmonella enterica subsp. enterica genomes were compared to identify potential associations with the host-adapted invasive serovars Typhi, Dublin, Choleraesuis, and Gallinarum, and with the broad host-range intestinal serovars Typhimurium, Enteritidis, and Newport. Results Through a bioinformatics analysis of 500 Salmonella genomes, we have identified allelic variants of 70 VFs, many of which are associated with either one of the four host-adapted invasive Salmonella serovars or one of the three broad host-range intestinal serovars. In addition, associations between specific VF alleles and intra-serovar clusters, sequence types (STs) and/or host-adapted FimH adhesins were identified. Moreover, new allelic VF associations with non-typhoidal S. Enteritidis and S. Typhimurium (NTS) or invasive NTS (iNTS) were detected. Conclusions By analogy to the previously shown association of specific FimH adhesin alleles with optimal binding by host adapted Salmonella serovars, lineages or strains, we predict that some of the identified association of other VF alleles with host-adapted serovars, lineages or strains will reflect specific contributions to host adaptation and/or pathogenesis. The identification of these allelic associations will support investigations of the biological impact of VF alleles and better characterize the role of allelic variation in Salmonella pathogenesis. Most relevant functional experiments will test the potential causal contribution of the detected FimH-associated VF variants in host adapted virulence. |
topic |
Salmonella enterica virulence factors allelic variation host adaptation FimH STs |
url |
http://link.springer.com/article/10.1186/s12864-019-5809-8 |
work_keys_str_mv |
AT alexeyvrakov associationofsalmonellavirulencefactoralleleswithintestinalandinvasiveserovars AT emiliomastriani associationofsalmonellavirulencefactoralleleswithintestinalandinvasiveserovars AT shulinliu associationofsalmonellavirulencefactoralleleswithintestinalandinvasiveserovars AT dietermschifferli associationofsalmonellavirulencefactoralleleswithintestinalandinvasiveserovars |
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