Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.
Transitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell dea...
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2012-01-01
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doaj-c0a317a043604adc920c40d0bc178d2a2021-03-03T20:26:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4891410.1371/journal.pone.0048914Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus.Leo W BussChristopher AndersonErica WestermanChad KritzbergerMonita PoudyalMaria A MorenoFadi G LakkisTransitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell death resulting in separation of the two incompatible colonies. We here characterize this cell death process using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and continuous in vivo digital microscopy. These techniques reveal widespread autophagy and subsequent necrosis in both colony and grafted polyp assays. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays and ultrastructural observations revealed no evidence of apoptosis. Pharmacological inhibition of autophagy using 3-methyladenine (3-MA) completely suppressed transitory fusion in vivo in colony assays. Rapamycin did not have a significant effect in the same assays. These results establish the hydroid allorecognition system as a novel model for the study of cell death.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23145018/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis |
spellingShingle |
Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. PLoS ONE |
author_facet |
Leo W Buss Christopher Anderson Erica Westerman Chad Kritzberger Monita Poudyal Maria A Moreno Fadi G Lakkis |
author_sort |
Leo W Buss |
title |
Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. |
title_short |
Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. |
title_full |
Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. |
title_fullStr |
Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. |
title_full_unstemmed |
Allorecognition triggers autophagy and subsequent necrosis in the cnidarian Hydractinia symbiolongicarpus. |
title_sort |
allorecognition triggers autophagy and subsequent necrosis in the cnidarian hydractinia symbiolongicarpus. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Transitory fusion is an allorecognition phenotype displayed by the colonial hydroid Hydractinia symbiolongicarpus when interacting colonies share some, but not all, loci within the allorecognition gene complex (ARC). The phenotype is characterized by an initial fusion followed by subsequent cell death resulting in separation of the two incompatible colonies. We here characterize this cell death process using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and continuous in vivo digital microscopy. These techniques reveal widespread autophagy and subsequent necrosis in both colony and grafted polyp assays. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays and ultrastructural observations revealed no evidence of apoptosis. Pharmacological inhibition of autophagy using 3-methyladenine (3-MA) completely suppressed transitory fusion in vivo in colony assays. Rapamycin did not have a significant effect in the same assays. These results establish the hydroid allorecognition system as a novel model for the study of cell death. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23145018/pdf/?tool=EBI |
work_keys_str_mv |
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