FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling
FAM172A, as a newly discovered gene, is little known in cancer development, especially in pancreatic cancer (PC). We investigated the potential role and molecular mechanism of FAM172A in epithelial to mesenchymal transition (EMT) in both human clinical samples and PC cells. FAM172A was downregulated...
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doaj-c0a37c2a06aa4c14822e3b020a39830e2021-06-02T15:44:21ZengThe Company of BiologistsBiology Open2046-63902020-02-019210.1242/bio.048462048462FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signalingYing Chen0Peihui Liu1Di Shen2Han Liu3Lepeng Xu4Jian Wang5Daguang Shen6He Sun7Hongkui Wu8 Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China Department of Intervention Therapy and Vascular Surgery, The Central Hospital of Huludao City, Huludao City, Liaoning Province, 125399 China FAM172A, as a newly discovered gene, is little known in cancer development, especially in pancreatic cancer (PC). We investigated the potential role and molecular mechanism of FAM172A in epithelial to mesenchymal transition (EMT) in both human clinical samples and PC cells. FAM172A was downregulated in human PC tissues compared with that in non-cancerous pancreas cells by immunohistochemistry and qRT-PCR. FAM172A expression was negatively associated with tumor size (P=0.015), T stage (P=0.006), lymph node metastasis (P=0.028) and the worst prognosis of PC patients (P=0.004). Meanwhile, a positive relationship between FAM172A and E-cadherin (E-cad) (r=0.381, P=0.002) was observed in clinical samples, which contributed to the better prognosis of PC patients (P=0.014). FAM172A silencing induced EMT in both AsPC-1 and BxPC-3 cells, including inducing the increase of Vimentin, MMP9 and pERK and the decrease of E-cad and β-catenin expression, stimulating EMT-like cell morphology and enhancing cell invasion and migration in PC cells. However, MEK1 inhibitor PD98059 reversed FAM172A silencing-enhanced EMT in PC cells. We conclude that FAM172A inhibits EMT of PC cells via ERK-MAPK signaling.http://bio.biologists.org/content/9/2/bio048462fam172aemtpancreatic cancererk-mapk |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ying Chen Peihui Liu Di Shen Han Liu Lepeng Xu Jian Wang Daguang Shen He Sun Hongkui Wu |
spellingShingle |
Ying Chen Peihui Liu Di Shen Han Liu Lepeng Xu Jian Wang Daguang Shen He Sun Hongkui Wu FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling Biology Open fam172a emt pancreatic cancer erk-mapk |
author_facet |
Ying Chen Peihui Liu Di Shen Han Liu Lepeng Xu Jian Wang Daguang Shen He Sun Hongkui Wu |
author_sort |
Ying Chen |
title |
FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling |
title_short |
FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling |
title_full |
FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling |
title_fullStr |
FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling |
title_full_unstemmed |
FAM172A inhibits EMT in pancreatic cancer via ERK-MAPK signaling |
title_sort |
fam172a inhibits emt in pancreatic cancer via erk-mapk signaling |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2020-02-01 |
description |
FAM172A, as a newly discovered gene, is little known in cancer development, especially in pancreatic cancer (PC). We investigated the potential role and molecular mechanism of FAM172A in epithelial to mesenchymal transition (EMT) in both human clinical samples and PC cells. FAM172A was downregulated in human PC tissues compared with that in non-cancerous pancreas cells by immunohistochemistry and qRT-PCR. FAM172A expression was negatively associated with tumor size (P=0.015), T stage (P=0.006), lymph node metastasis (P=0.028) and the worst prognosis of PC patients (P=0.004). Meanwhile, a positive relationship between FAM172A and E-cadherin (E-cad) (r=0.381, P=0.002) was observed in clinical samples, which contributed to the better prognosis of PC patients (P=0.014). FAM172A silencing induced EMT in both AsPC-1 and BxPC-3 cells, including inducing the increase of Vimentin, MMP9 and pERK and the decrease of E-cad and β-catenin expression, stimulating EMT-like cell morphology and enhancing cell invasion and migration in PC cells. However, MEK1 inhibitor PD98059 reversed FAM172A silencing-enhanced EMT in PC cells. We conclude that FAM172A inhibits EMT of PC cells via ERK-MAPK signaling. |
topic |
fam172a emt pancreatic cancer erk-mapk |
url |
http://bio.biologists.org/content/9/2/bio048462 |
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