Colorimetric Quantification Methods for Peracetic Acid together with Hydrogen Peroxide for Water Disinfection Process Control

• Main Authors: Ravi Kumar Chhetri, Kamilla Marie Speht Kaarsholm, Henrik Rasmus Andersen
• Format: Article
• Language: English
• Published: MDPI AG 2020-06-01
• Series: International Journal of Environmental Research and Public Health
• Subjects: peracetic acid; 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS); N,N-diethyl-p-phenylenediamine (DPD); disinfection; Hydrogen peroxide
• Online Access: https://www.mdpi.com/1660-4601/17/13/4656

Peracetic acid (PAA) water solutions is applied for disinfection of industry systems, food products and non-potable water. Commercially available peracetic acid is always supplied mixed with hydrogen peroxide (H₂O₂). H₂O₂ degrade slower than the peracetic acid which creates a need to quantify both peroxides separately to gauge the disinfection power of the solution and the residuals. Two combinations of colorimetric reactions are presented that allows simultaneous quantification at the mg·L⁻¹level used in disinfection liquids and water disinfection. The first dichromic reaction use titanium oxide oxalate (TiO-Ox) which only react with H₂O₂ followed by addition of N,N-diethyl-p-phenylenediamine with iodide (DPD/I⁻) and the concentrations are read by simultaneously measuring the absorbance at 400 and 515 nm. Limit of quantification (LOQ) and maximal concentration determined was 4.6 µg·L⁻¹and 2.5 mg·L⁻¹ for PAA and 9.1 µg·L⁻¹ and 5 mg·L⁻¹ for H₂O₂. The two color reactions didn’t interfere with each other when the reagent addition was consecutive. Another combination of colorimetric reaction also used where TiO-Ox was used to first measure H₂O₂ at 400 nm, before addition of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS)) and reading the absorbance at 405 nm. ABTS changes the absorbance at 405 nm necessitating the two measurements be done separately. LOQ and maximal concentration determined using ABTS colorimetric assay was 42.5 µg·L⁻¹ and 30 mg·L⁻¹ for PAA and for titanium oxide oxalate colorimetric assay was 12.7 µg·L⁻¹and 75 mg·L⁻¹ for H₂O₂. Both methods tested satisfactory in typical water samples (Tap, sea, lake, and biological treated sewage) spiked with peracetic acid and H₂O₂, separately.