Isolation frequency of Mycobacterium tuberculosis in tertiary reference hospital and their susceptibility patterns against four major anti-tuberculosis drugs

• Main Authors: Teoman Apan, Tevfik Pınar, Altan Aksoy, Aydanur Ekici, Aytül Çakmak, J. Sedef Göçmen, Meral Saygun, Latife İşeri Abut
• Format: Article
• Language: English
• Published: Modestum Publishing LTD 2012-06-01
• Series: Journal of Clinical and Experimental Investigations
• Subjects: Tuberculosis; culture techniques; drug resistance
• Online Access: http://jceionline.org/upload/sayi/12/JCEI-00376.pdf
• ISSN: 1309-8578; 1309-6621

Objectives: As soon as possible to detection of tuberculosisbacilli and to determination of their anti-tuberculosisdrug susceptibilities are very important for an optimalanti-tuberculosis therapy. Therefore, two different culturemethods in suspected patient samples were performed.The results of anti-tuberculosis drug susceptibility testingof culture positive samples were examined.Materials and methods: Following the homogenizationand decontamination processes, patient’s samples werestained with Ziehl-Neelsen dye and each preparationwere inoculated separately onto Lowenstein-Jensen (LJ)and into Mycobacterium Growth Indicator Tube (MGIT)media. After incubation period, drug susceptibilities of bacillusin culture positive samples against four major antituberculosisdrugs were investigated.Results: The samples obtained from 163 different patientsbetween 2007 and 2010 years were evaluated.In all samples, it was determined that positivity of AcidResistant Bacillus (ARB), MGIT and LJ media were 7(4.0%), 6 (3.5%) and 7 (4.0%), respectively. MGIT methodwas demonstrated significantly earlier growth than LJmethod the tuberculosis bacillus. All isolates were foundto be sensitive to four major anti-tuberculosis drugs.Conclusion: In our study, MGIT method was found to besuperior to LJ media because of its quick result and drugresistance was not determined in all isolates. J Clin ExpInvest 2012; 3(2): 240-244