Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density
Drought is one of the most prevalent environmental stresses that affect plant growth and development. Improvement in drought tolerance is associated with reduced stomatal density and higher water use efficiency (WUE). In this study, an epidermal patterning factor (EPF), PdEPF3, from a fast-growing p...
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Polish Botanical Society
2019-06-01
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doaj-c0c0bfdb42c84d809a8b66b00e75a31f2020-11-25T03:03:23ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802019-06-0188210.5586/asbp.36276989Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal densitySha Liu0Fuli Jia1Zhiyin Jiao2Jingjing Wang3Xinli Xia4Weilun Yin5College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056021; National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083Drought is one of the most prevalent environmental stresses that affect plant growth and development. Improvement in drought tolerance is associated with reduced stomatal density and higher water use efficiency (WUE). In this study, an epidermal patterning factor (EPF), PdEPF3, from a fast-growing poplar clone, NE-19 [Populus nigra × (Populus deltoides × Populus nigra)], was characterized. Quantitative reverse transcription polymerase chain reaction showed that the transcription of PdEPF3 was induced by drought. We further found that the transgenic Arabidopsis overexpressing PdEPF3 had an earlier seedling germination and longer primary roots under osmotic stress treatments, compared with the WT and mutant epf1-1. In addition, ectopic overexpression of PdEPF3 in Arabidopsis plants was able to enhance drought tolerance. This observation was associated with the reduced stomatal density of transgenic plants, which would limit transpiration and reduce water loss, consequently improving the WUE of plants. Interestingly, the reduction of stomatal density in transgenic plants overexpressing PdEPF3 did not affect their photosynthetic capacity. These results indicate that PdEPF3 could be used in transgenic breeding to enhance plant drought tolerance.https://pbsociety.org.pl/journals/index.php/asbp/article/view/8077droughtPdEPF3stomatal densitywater use efficiency (WUE) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sha Liu Fuli Jia Zhiyin Jiao Jingjing Wang Xinli Xia Weilun Yin |
spellingShingle |
Sha Liu Fuli Jia Zhiyin Jiao Jingjing Wang Xinli Xia Weilun Yin Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density Acta Societatis Botanicorum Poloniae drought PdEPF3 stomatal density water use efficiency (WUE) |
author_facet |
Sha Liu Fuli Jia Zhiyin Jiao Jingjing Wang Xinli Xia Weilun Yin |
author_sort |
Sha Liu |
title |
Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density |
title_short |
Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density |
title_full |
Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density |
title_fullStr |
Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density |
title_full_unstemmed |
Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density |
title_sort |
ectopic expression of secretory peptide pdepf3 in arabidopsis confers drought tolerance with reduced stomatal density |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2019-06-01 |
description |
Drought is one of the most prevalent environmental stresses that affect plant growth and development. Improvement in drought tolerance is associated with reduced stomatal density and higher water use efficiency (WUE). In this study, an epidermal patterning factor (EPF), PdEPF3, from a fast-growing poplar clone, NE-19 [Populus nigra × (Populus deltoides × Populus nigra)], was characterized. Quantitative reverse transcription polymerase chain reaction showed that the transcription of PdEPF3 was induced by drought. We further found that the transgenic Arabidopsis overexpressing PdEPF3 had an earlier seedling germination and longer primary roots under osmotic stress treatments, compared with the WT and mutant epf1-1. In addition, ectopic overexpression of PdEPF3 in Arabidopsis plants was able to enhance drought tolerance. This observation was associated with the reduced stomatal density of transgenic plants, which would limit transpiration and reduce water loss, consequently improving the WUE of plants. Interestingly, the reduction of stomatal density in transgenic plants overexpressing PdEPF3 did not affect their photosynthetic capacity. These results indicate that PdEPF3 could be used in transgenic breeding to enhance plant drought tolerance. |
topic |
drought PdEPF3 stomatal density water use efficiency (WUE) |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/8077 |
work_keys_str_mv |
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