Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections
Abstract Recent advancements in DNA-based approaches have led to the identification of uncommon and rare bacterial pathogens. In this study, by utilizing a DNA-based approach, a total of 1043 clinical specimens were processed for the identification of actinobacteria targeting the 16S rRNA and gyrB g...
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doaj-c0d02cb7f6344da88be57552bd0e2ee22021-05-30T11:33:41ZengNature Publishing GroupScientific Reports2045-23222021-05-011111910.1038/s41598-021-90269-5Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infectionsHossein Ali Rahdar0Shahram Mahmoudi1Abbas Bahador2Fereshteh Ghiasvand3Fatemah Sadeghpour Heravi4Mohammad Mehdi Feizabadi5Department of Microbiology, School of Medicine, Iranshahr University of Medical SciencesDepartment of Parasitology and Mycology, School of Medicine, Iran University of Medical SciencesDepartment of Medical Microbiology, School of Medicine, Tehran University of Medical SciencesDepartment of Infectious Diseases, Imam Khomeini Hospital Complex, Tehran University of Medical SciencesSurgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie UniversityDepartment of Medical Microbiology, School of Medicine, Tehran University of Medical SciencesAbstract Recent advancements in DNA-based approaches have led to the identification of uncommon and rare bacterial pathogens. In this study, by utilizing a DNA-based approach, a total of 1043 clinical specimens were processed for the identification of actinobacteria targeting the 16S rRNA and gyrB genes. Drug susceptibility testing was also conducted using micro-broth dilution and PCR. Two isolates of Nocardia flavorosea and Rhodococcus erythropolis were reported for the first time in Iran. Also, Nocardiopsis dassonvillei, Streptomyces olivaceus, and Streptomyces griseus were reported for the first time in Asia. Infections caused by Nocardia caishijiensis and Prauserella muralis have also been reported in this study. The first Asian case of pulmonary infection caused by Nocardia ignorata and the first global case of brain abscess caused by Nocardia ninae and Nocardia neocaledoniensis have been reported in this study. Overall 30 isolates belonging to 6 genera (Nocardia, Streptomyces, Rodoccoccus, Nocardiopsis, Rothia, and Prauserella) were detected in 30 patients. All 30 isolates were susceptible to amikacin and linezolid. Three isolates including Nocardia otitidiscaviarum (n = 2) and Nocardia flavorosea (n = 1) were resistant to trimethoprim-sulfamethoxazole which were the first trimethoprim-sulfamethoxazole resistant clinical actinomycetes in Iran. Isolation of rare species of actinomycetes particularly Nocardia spp. requires urgent action before they spread clinically particularly among immunocompromised patients.https://doi.org/10.1038/s41598-021-90269-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hossein Ali Rahdar Shahram Mahmoudi Abbas Bahador Fereshteh Ghiasvand Fatemah Sadeghpour Heravi Mohammad Mehdi Feizabadi |
spellingShingle |
Hossein Ali Rahdar Shahram Mahmoudi Abbas Bahador Fereshteh Ghiasvand Fatemah Sadeghpour Heravi Mohammad Mehdi Feizabadi Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections Scientific Reports |
author_facet |
Hossein Ali Rahdar Shahram Mahmoudi Abbas Bahador Fereshteh Ghiasvand Fatemah Sadeghpour Heravi Mohammad Mehdi Feizabadi |
author_sort |
Hossein Ali Rahdar |
title |
Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections |
title_short |
Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections |
title_full |
Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections |
title_fullStr |
Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections |
title_full_unstemmed |
Molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in Iran, emerging of new infections |
title_sort |
molecular identification and antibiotic resistance pattern of actinomycetes isolates among immunocompromised patients in iran, emerging of new infections |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract Recent advancements in DNA-based approaches have led to the identification of uncommon and rare bacterial pathogens. In this study, by utilizing a DNA-based approach, a total of 1043 clinical specimens were processed for the identification of actinobacteria targeting the 16S rRNA and gyrB genes. Drug susceptibility testing was also conducted using micro-broth dilution and PCR. Two isolates of Nocardia flavorosea and Rhodococcus erythropolis were reported for the first time in Iran. Also, Nocardiopsis dassonvillei, Streptomyces olivaceus, and Streptomyces griseus were reported for the first time in Asia. Infections caused by Nocardia caishijiensis and Prauserella muralis have also been reported in this study. The first Asian case of pulmonary infection caused by Nocardia ignorata and the first global case of brain abscess caused by Nocardia ninae and Nocardia neocaledoniensis have been reported in this study. Overall 30 isolates belonging to 6 genera (Nocardia, Streptomyces, Rodoccoccus, Nocardiopsis, Rothia, and Prauserella) were detected in 30 patients. All 30 isolates were susceptible to amikacin and linezolid. Three isolates including Nocardia otitidiscaviarum (n = 2) and Nocardia flavorosea (n = 1) were resistant to trimethoprim-sulfamethoxazole which were the first trimethoprim-sulfamethoxazole resistant clinical actinomycetes in Iran. Isolation of rare species of actinomycetes particularly Nocardia spp. requires urgent action before they spread clinically particularly among immunocompromised patients. |
url |
https://doi.org/10.1038/s41598-021-90269-5 |
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