LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III
Stinkvine (Paederia foetida L.) is known as a plant that has many properties that are empirically used in traditional medicine. However, information of utilization of Stinkvine leaf outside as a traditional medicine has not been discovered yet. This research aims to determine the potential larvicida...
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Universitas Gadjah Mada
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doaj-c0d2552bc35e4acf9e23101105e78a6a2020-11-24T22:51:22ZengUniversitas Gadjah MadaMajalah Obat Tradisional1410-59182406-90862016-12-0121313714210.22146/tradmedj.1730711775LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR IIIRollando Rollando0Maywan Hariono1Program Farmasi, Fakultas Sains dan Teknologi, Universitas Ma ChungFakultas Farmasi, Universitas Sanata DarmaStinkvine (Paederia foetida L.) is known as a plant that has many properties that are empirically used in traditional medicine. However, information of utilization of Stinkvine leaf outside as a traditional medicine has not been discovered yet. This research aims to determine the potential larvicidal extracts and fractions of stinkvine leaf. The ethanol extract of stinkvine leaf fractionated using column chromatography with n-hexane, ethyl acetate, and methanol. Larvicidal activity test against Aedes aegypti and Anopheles III instar was done using 25 larvae for each treatment extracts and fractions in 5 series of concentration, and left exposed for 24 hours. Total mortality of larvae is calculated and analyzed using a modified Finney probit to determine the LC50 and LC90 values. The results showed larvicidal activity on larvae of Aedes aegypti mosquitoes with LC50 and LC90 value of n-hexane, ethyl acetate, and methanol fraction respectively 67,89 ; 10,92 ; 30,98 µg/mL and 114,11 ; 18,80 ; 54, 12 µg/mL. Larvicidal activity on larvae of Anopheles mosquitoes with LC50 and LC90 value of n-hexane, ethyl acetate, and methanol fraction respectively 50,76 ; 14,96 ; 60,82 µg/mL and 87,23 ; 25,65 ; 90,92 µg/mL. Ethyl acetate fraction of ethanol extract of stinkvine leaf (Paederia foetida L.) shows to have highest larvicidal activity against the mosquito larvae of Aedes aegypti and Anopheles. The results of GC-MS analysis of the ethyl acetate fraction showed there are 15 compounds, a constituent component consisting of lupeol (20,32 %), gamma-sitosterol (12,22 %), cyclohexanecarboxamide (11,82 %), campesterol (11,45 %), 3-butenol, 4- (2,6,6-trimethyl-1-sikloheksenil) (7,98 %), siklolanos-24-en-3-ol (7,87%), and there are 9 other components with a percentage amount of 0,18 to 5,86 %.https://jurnal.ugm.ac.id/TradMedJ/article/view/17307Daun sembukan, fraksi etil asetat, larvasida, Aedes aegypti, Anopheles |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rollando Rollando Maywan Hariono |
spellingShingle |
Rollando Rollando Maywan Hariono LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III Majalah Obat Tradisional Daun sembukan, fraksi etil asetat, larvasida, Aedes aegypti, Anopheles |
author_facet |
Rollando Rollando Maywan Hariono |
author_sort |
Rollando Rollando |
title |
LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III |
title_short |
LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III |
title_full |
LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III |
title_fullStr |
LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III |
title_full_unstemmed |
LARVICIDAL ACTIVITY OF ETHANOL EXTRACT, N-HEXANE FRACTION, ETHYL ACETATE, AND METHANOL EXTRACT FROM STINKVINE LEAF (Paederia foetida L.) AGAINST LARVAE OF Aedes aegypti AND Anopheles INSTAR III |
title_sort |
larvicidal activity of ethanol extract, n-hexane fraction, ethyl acetate, and methanol extract from stinkvine leaf (paederia foetida l.) against larvae of aedes aegypti and anopheles instar iii |
publisher |
Universitas Gadjah Mada |
series |
Majalah Obat Tradisional |
issn |
1410-5918 2406-9086 |
publishDate |
2016-12-01 |
description |
Stinkvine (Paederia foetida L.) is known as a plant that has many properties that are empirically used in traditional medicine. However, information of utilization of Stinkvine leaf outside as a traditional medicine has not been discovered yet. This research aims to determine the potential larvicidal extracts and fractions of stinkvine leaf. The ethanol extract of stinkvine leaf fractionated using column chromatography with n-hexane, ethyl acetate, and methanol. Larvicidal activity test against Aedes aegypti and Anopheles III instar was done using 25 larvae for each treatment extracts and fractions in 5 series of concentration, and left exposed for 24 hours. Total mortality of larvae is calculated and analyzed using a modified Finney probit to determine the LC50 and LC90 values. The results showed larvicidal activity on larvae of Aedes aegypti mosquitoes with LC50 and LC90 value of n-hexane, ethyl acetate, and methanol fraction respectively 67,89 ; 10,92 ; 30,98 µg/mL and 114,11 ; 18,80 ; 54, 12 µg/mL. Larvicidal activity on larvae of Anopheles mosquitoes with LC50 and LC90 value of n-hexane, ethyl acetate, and methanol fraction respectively 50,76 ; 14,96 ; 60,82 µg/mL and 87,23 ; 25,65 ; 90,92 µg/mL. Ethyl acetate fraction of ethanol extract of stinkvine leaf (Paederia foetida L.) shows to have highest larvicidal activity against the mosquito larvae of Aedes aegypti and Anopheles. The results of GC-MS analysis of the ethyl acetate fraction showed there are 15 compounds, a constituent component consisting of lupeol (20,32 %), gamma-sitosterol (12,22 %), cyclohexanecarboxamide (11,82 %), campesterol (11,45 %), 3-butenol, 4- (2,6,6-trimethyl-1-sikloheksenil) (7,98 %), siklolanos-24-en-3-ol (7,87%), and there are 9 other components with a percentage amount of 0,18 to 5,86 %. |
topic |
Daun sembukan, fraksi etil asetat, larvasida, Aedes aegypti, Anopheles |
url |
https://jurnal.ugm.ac.id/TradMedJ/article/view/17307 |
work_keys_str_mv |
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