Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3
Plants encrypt the perception of different pathogenic stimuli into specific intracellular calcium (Ca<sup>2+</sup>) signatures and subsequently decrypt the signatures into appropriate downstream responses through various Ca<sup>2+</sup> sensors. Two microbe-associated molecul...
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doaj-c0df5ba7e5cb43ea9c7f11059b117d8a2020-11-25T04:04:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01218163816310.3390/ijms21218163Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3Peiguo Yuan0Jeremy B. Jewell1Smrutisanjita Behera2Kiwamu Tanaka3B. W. Poovaiah4Laboratory of Molecular Plant Science, Department of Horticulture, Washington State University, Pullman, WA 99164-6414, USADepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USAIndian Institute of Chemical Biology, Kolkata, West Bengal 700 032, IndiaDepartment of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USALaboratory of Molecular Plant Science, Department of Horticulture, Washington State University, Pullman, WA 99164-6414, USAPlants encrypt the perception of different pathogenic stimuli into specific intracellular calcium (Ca<sup>2+</sup>) signatures and subsequently decrypt the signatures into appropriate downstream responses through various Ca<sup>2+</sup> sensors. Two microbe-associated molecular patterns (MAMPs), bacterial flg22 and fungal chitin, and one damage-associated molecular pattern (DAMP), AtPep1, were used to study the differential Ca<sup>2+</sup> signatures in <i>Arabidopsis</i> leaves. The results revealed that flg22, chitin, and AtPep1 induced distinct changes in Ca<sup>2+</sup> dynamics in both the cytosol and nucleus. In addition, Flg22 and chitin upregulated the expression of salicylic acid-related genes, <i>ICS1</i> and <i>EDS1</i>, whereas AtPep1 upregulated the expression of jasmonic acid-related genes, <i>JAZ1</i> and <i>PDF1.2</i>, in addition to <i>ICS1</i> and <i>EDS1.</i> These data demonstrated that distinct Ca<sup>2+</sup> signatures caused by different molecular patterns in leaf cells lead to specific downstream events. Furthermore, these changes in the expression of defense-related genes were disrupted in a knockout mutant of the <i>AtSR1/CAMTA3</i> gene, encoding a calmodulin-binding transcription factor, in which a calmodulin-binding domain on AtSR1 was required for deciphering the Ca<sup>2+</sup> signatures into downstream transcription events. These observations extend our knowledge regarding unique and intrinsic roles for Ca<sup>2+</sup> signaling in launching and fine-tuning plant immune response, which are mediated by the AtSR1/CAMTA3 transcription factor.https://www.mdpi.com/1422-0067/21/21/8163MAMPsDAMPsnuclear and cytoplasmic calcium signalingplant immune responsesalicylic acidjasmonic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peiguo Yuan Jeremy B. Jewell Smrutisanjita Behera Kiwamu Tanaka B. W. Poovaiah |
spellingShingle |
Peiguo Yuan Jeremy B. Jewell Smrutisanjita Behera Kiwamu Tanaka B. W. Poovaiah Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 International Journal of Molecular Sciences MAMPs DAMPs nuclear and cytoplasmic calcium signaling plant immune response salicylic acid jasmonic acid |
author_facet |
Peiguo Yuan Jeremy B. Jewell Smrutisanjita Behera Kiwamu Tanaka B. W. Poovaiah |
author_sort |
Peiguo Yuan |
title |
Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 |
title_short |
Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 |
title_full |
Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 |
title_fullStr |
Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 |
title_full_unstemmed |
Distinct Molecular Pattern-Induced Calcium Signatures Lead to Different Downstream Transcriptional Regulations via AtSR1/CAMTA3 |
title_sort |
distinct molecular pattern-induced calcium signatures lead to different downstream transcriptional regulations via atsr1/camta3 |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
Plants encrypt the perception of different pathogenic stimuli into specific intracellular calcium (Ca<sup>2+</sup>) signatures and subsequently decrypt the signatures into appropriate downstream responses through various Ca<sup>2+</sup> sensors. Two microbe-associated molecular patterns (MAMPs), bacterial flg22 and fungal chitin, and one damage-associated molecular pattern (DAMP), AtPep1, were used to study the differential Ca<sup>2+</sup> signatures in <i>Arabidopsis</i> leaves. The results revealed that flg22, chitin, and AtPep1 induced distinct changes in Ca<sup>2+</sup> dynamics in both the cytosol and nucleus. In addition, Flg22 and chitin upregulated the expression of salicylic acid-related genes, <i>ICS1</i> and <i>EDS1</i>, whereas AtPep1 upregulated the expression of jasmonic acid-related genes, <i>JAZ1</i> and <i>PDF1.2</i>, in addition to <i>ICS1</i> and <i>EDS1.</i> These data demonstrated that distinct Ca<sup>2+</sup> signatures caused by different molecular patterns in leaf cells lead to specific downstream events. Furthermore, these changes in the expression of defense-related genes were disrupted in a knockout mutant of the <i>AtSR1/CAMTA3</i> gene, encoding a calmodulin-binding transcription factor, in which a calmodulin-binding domain on AtSR1 was required for deciphering the Ca<sup>2+</sup> signatures into downstream transcription events. These observations extend our knowledge regarding unique and intrinsic roles for Ca<sup>2+</sup> signaling in launching and fine-tuning plant immune response, which are mediated by the AtSR1/CAMTA3 transcription factor. |
topic |
MAMPs DAMPs nuclear and cytoplasmic calcium signaling plant immune response salicylic acid jasmonic acid |
url |
https://www.mdpi.com/1422-0067/21/21/8163 |
work_keys_str_mv |
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