Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases
To verify the idea that extracellular free oligosaccharides might be able to reflect the functional status of the endoplasmic reticulum (ER) and lysosomal-endosomal system, HPLC-profiles of serum-derived free oligosaccharides (FOS) in human healthy aging, acute myeloproliferative neoplasms, and card...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2017-02-01
|
Series: | Ukrainian Biochemical Journal |
Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Pismenetska_1_17.pdf |
id |
doaj-c0e483c046844e178942ba2f3462dfe6 |
---|---|
record_format |
Article |
spelling |
doaj-c0e483c046844e178942ba2f3462dfe62020-11-24T22:38:08ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Ukrainian Biochemical Journal2409-49432413-50032017-02-01891597010.15407/ubj89.01.059Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseasesI. U. Pismenetskaya0T. D. Butters1SI Dnepropetrovsk Medical Academy, UkraineCarboNet Consulting Ltd., Oxford, UKTo verify the idea that extracellular free oligosaccharides might be able to reflect the functional status of the endoplasmic reticulum (ER) and lysosomal-endosomal system, HPLC-profiles of serum-derived free oligosaccharides (FOS) in human healthy aging, acute myeloproliferative neoplasms, and cardiovascular pathologies were compared with intracellular glycans. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid, separated into the neutral and charged with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). The charged FOS were digested with a sialidase and compared with free oligosaccharides from transferrin for structural decoding. HPLC-profiles of serum-derived FOS revealed mild delay of the dolichol phosphate cycle in ER, moderate intensification of ER-associated degradation (ERAD) and degradation in endosomal-lysosomal system with aging; an inhibition of the dolichol phosphate cycle, intensification of ERAD and increasing of lysosomal exocytosis in acute myeloproliferative neoplasms; intensification of ERAD and glycocojugate degradation with endosomal-lysosomal system in cardiovascular diseases. As serum free oligosaccharides are able to reflect specifically perturbations in ER and endosomal-lysosomal system under wide range of stressors they can serve as extracellular markers of functionality of these organelles.http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Pismenetska_1_17.pdf |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
I. U. Pismenetskaya T. D. Butters |
spellingShingle |
I. U. Pismenetskaya T. D. Butters Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases Ukrainian Biochemical Journal |
author_facet |
I. U. Pismenetskaya T. D. Butters |
author_sort |
I. U. Pismenetskaya |
title |
Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
title_short |
Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
title_full |
Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
title_fullStr |
Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
title_full_unstemmed |
Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
title_sort |
serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Ukrainian Biochemical Journal |
issn |
2409-4943 2413-5003 |
publishDate |
2017-02-01 |
description |
To verify the idea that extracellular free oligosaccharides might be able to reflect the functional status of the endoplasmic reticulum (ER) and lysosomal-endosomal system, HPLC-profiles of serum-derived free oligosaccharides (FOS) in human healthy aging, acute myeloproliferative neoplasms, and cardiovascular pathologies were compared with intracellular glycans. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid, separated into the neutral and charged with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). The charged FOS were digested with a sialidase and compared with free oligosaccharides from transferrin for structural decoding. HPLC-profiles of serum-derived FOS revealed mild delay of the dolichol phosphate cycle in ER, moderate intensification of ER-associated degradation (ERAD) and degradation in endosomal-lysosomal system with aging; an inhibition of the dolichol phosphate cycle, intensification of ERAD and increasing of lysosomal exocytosis in acute myeloproliferative neoplasms; intensification of ERAD and glycocojugate degradation with endosomal-lysosomal system in cardiovascular diseases. As serum free oligosaccharides are able to reflect specifically perturbations in ER and endosomal-lysosomal system under wide range of stressors they can serve as extracellular markers of functionality of these organelles. |
url |
http://ukrbiochemjournal.org/wp-content/uploads/2017/01/Pismenetska_1_17.pdf |
work_keys_str_mv |
AT iupismenetskaya serumglycomarkersofendoplasmicreticulumandlysosomalendosomalsystemstressinhumanhealthyaginganddiseases AT tdbutters serumglycomarkersofendoplasmicreticulumandlysosomalendosomalsystemstressinhumanhealthyaginganddiseases |
_version_ |
1725714562934636544 |