Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women
Abstract Background Vulvar squamous cell carcinoma (VSCC) is a rare malignancy of the female genital tract. We aimed to determine the mucosal high-risk human papillomavirus (HPV)-attributable fraction of VSCCs from Italian women using multiple markers of viral infections. Methods VSCCs and 8 metasta...
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doaj-c0f90eaad7be4d0e93b9afe5ef7d8a792020-11-25T03:04:06ZengBMCInfectious Agents and Cancer1750-93782020-04-011511810.1186/s13027-020-00286-8Role of human papillomavirus infection in the etiology of vulvar cancer in Italian womenMario Preti0John Charles Rotondo1Dana Holzinger2Leonardo Micheletti3Niccolò Gallio4Sandrine McKay-Chopin5Christine Carreira6Sebastiana Silvana Privitera7Reiko Watanabe8Ruediger Ridder9Michael Pawlita10Chiara Benedetto11Massimo Tommasino12Tarik Gheit13Department of Surgical Sciences, University of TurinInternational Agency for Research on CancerInfections and Cancer Epidemiology, Infections and Cancer Program, German Cancer Research Center (DKFZ)Department of Surgical Sciences, University of TurinDepartment of Surgical Sciences, University of TurinInternational Agency for Research on CancerInternational Agency for Research on CancerDepartment of Oncology, Città della Salute e della Scienza, University of TurinInternational Agency for Research on CancerRoche mtm laboratoriesInfections and Cancer Epidemiology, Infections and Cancer Program, German Cancer Research Center (DKFZ)Department of Surgical Sciences, University of TurinInternational Agency for Research on CancerInternational Agency for Research on CancerAbstract Background Vulvar squamous cell carcinoma (VSCC) is a rare malignancy of the female genital tract. We aimed to determine the mucosal high-risk human papillomavirus (HPV)-attributable fraction of VSCCs from Italian women using multiple markers of viral infections. Methods VSCCs and 8 metastatic lymph node samples from 107 Italian women were analyzed by a highly type-specific multiplex genotyping assay for the presence of DNA from 119 different HPVs. Tissues were further analyzed for HPV RNA and for upregulation of the cellular protein p16INK4a. Results The rate of mucosal HPV-related tumors defined by viral DNA and RNA positivity was low (7.8%). HPV16 was the most prevalent, followed by 53, 56, and 58. Only five (4.9%) p16INK4a-positive tumors were also positive for both viral DNA and RNA. One (14.3%) metastatic lymph node sample was positive for all three markers. DNA of cutaneous HPVs was detected in only two VSCCs, i.e. genus beta types 5 and 110. Conclusion A small proportion of Italian VSCCs is putatively HPV-related, i.e. positive for both viral DNA and RNA of the same type, thus reinforcing the importance of HPV vaccination. Moreover, this study suggests that a direct role of HPV from genus beta and gamma in vulvar carcinogenesis is unlikely.http://link.springer.com/article/10.1186/s13027-020-00286-8CancerHuman papillomavirusLymph node metastatic tissuesVulvar squamous cell carcinomaMultiple markers of viral infections |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mario Preti John Charles Rotondo Dana Holzinger Leonardo Micheletti Niccolò Gallio Sandrine McKay-Chopin Christine Carreira Sebastiana Silvana Privitera Reiko Watanabe Ruediger Ridder Michael Pawlita Chiara Benedetto Massimo Tommasino Tarik Gheit |
spellingShingle |
Mario Preti John Charles Rotondo Dana Holzinger Leonardo Micheletti Niccolò Gallio Sandrine McKay-Chopin Christine Carreira Sebastiana Silvana Privitera Reiko Watanabe Ruediger Ridder Michael Pawlita Chiara Benedetto Massimo Tommasino Tarik Gheit Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women Infectious Agents and Cancer Cancer Human papillomavirus Lymph node metastatic tissues Vulvar squamous cell carcinoma Multiple markers of viral infections |
author_facet |
Mario Preti John Charles Rotondo Dana Holzinger Leonardo Micheletti Niccolò Gallio Sandrine McKay-Chopin Christine Carreira Sebastiana Silvana Privitera Reiko Watanabe Ruediger Ridder Michael Pawlita Chiara Benedetto Massimo Tommasino Tarik Gheit |
author_sort |
Mario Preti |
title |
Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women |
title_short |
Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women |
title_full |
Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women |
title_fullStr |
Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women |
title_full_unstemmed |
Role of human papillomavirus infection in the etiology of vulvar cancer in Italian women |
title_sort |
role of human papillomavirus infection in the etiology of vulvar cancer in italian women |
publisher |
BMC |
series |
Infectious Agents and Cancer |
issn |
1750-9378 |
publishDate |
2020-04-01 |
description |
Abstract Background Vulvar squamous cell carcinoma (VSCC) is a rare malignancy of the female genital tract. We aimed to determine the mucosal high-risk human papillomavirus (HPV)-attributable fraction of VSCCs from Italian women using multiple markers of viral infections. Methods VSCCs and 8 metastatic lymph node samples from 107 Italian women were analyzed by a highly type-specific multiplex genotyping assay for the presence of DNA from 119 different HPVs. Tissues were further analyzed for HPV RNA and for upregulation of the cellular protein p16INK4a. Results The rate of mucosal HPV-related tumors defined by viral DNA and RNA positivity was low (7.8%). HPV16 was the most prevalent, followed by 53, 56, and 58. Only five (4.9%) p16INK4a-positive tumors were also positive for both viral DNA and RNA. One (14.3%) metastatic lymph node sample was positive for all three markers. DNA of cutaneous HPVs was detected in only two VSCCs, i.e. genus beta types 5 and 110. Conclusion A small proportion of Italian VSCCs is putatively HPV-related, i.e. positive for both viral DNA and RNA of the same type, thus reinforcing the importance of HPV vaccination. Moreover, this study suggests that a direct role of HPV from genus beta and gamma in vulvar carcinogenesis is unlikely. |
topic |
Cancer Human papillomavirus Lymph node metastatic tissues Vulvar squamous cell carcinoma Multiple markers of viral infections |
url |
http://link.springer.com/article/10.1186/s13027-020-00286-8 |
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