The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1
Borealin is a key component of chromosomal passenger complex, which is vital in cytokinesis. IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis. The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear. Here, we used mass spectrometry an...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2020/6231697 |
id |
doaj-c11667258ce04165bc584b24346e7a2c |
---|---|
record_format |
Article |
spelling |
doaj-c11667258ce04165bc584b24346e7a2c2020-11-25T02:36:54ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/62316976231697The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1Reziya Wumaier0Abudunaibi Aili1Hexige Saiyin2Pingzhao Zhang3Lihuan Cao4Aikeremujiang Muheremu5State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, ChinaDepartment of Spine Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 86830001, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, ChinaDepartment of Orthopaedics, Peking University Third Hospital, Beijing 86100191, ChinaBorealin is a key component of chromosomal passenger complex, which is vital in cytokinesis. IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis. The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear. Here, we used mass spectrometry and endogenous coimmunoprecipitation experiments to investigate the interaction between IQGAP1 and Borealin. Results of the current study showed that Borealin interacted directly with IQGAP1 both in vitro and in vivo. Knockdown of IQGAP1 resulted in an abnormal location of Borealin in the midbody. Knocking down Borealin alone, IQGAP1 alone, or Borealin and IQGAP1 at the same time inhibited the completion of cytokinesis and formed multinucleated cells. Our results indicated that IQGAP1 interacts with Borealin during cytokinesis, and the correct localization of Borealin in the midbody during cytokinesis is determined by IQGAP1, and IQGAP1 may play an important role in regulating Borealin function in cytokinesis.http://dx.doi.org/10.1155/2020/6231697 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reziya Wumaier Abudunaibi Aili Hexige Saiyin Pingzhao Zhang Lihuan Cao Aikeremujiang Muheremu |
spellingShingle |
Reziya Wumaier Abudunaibi Aili Hexige Saiyin Pingzhao Zhang Lihuan Cao Aikeremujiang Muheremu The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 BioMed Research International |
author_facet |
Reziya Wumaier Abudunaibi Aili Hexige Saiyin Pingzhao Zhang Lihuan Cao Aikeremujiang Muheremu |
author_sort |
Reziya Wumaier |
title |
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 |
title_short |
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 |
title_full |
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 |
title_fullStr |
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 |
title_full_unstemmed |
The Correct Localization of Borealin in Midbody during Cytokinesis Depends on IQGAP1 |
title_sort |
correct localization of borealin in midbody during cytokinesis depends on iqgap1 |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Borealin is a key component of chromosomal passenger complex, which is vital in cytokinesis. IQ domain-containing GTPase-activating protein 1 (IQGAP1) also participates in cytokinesis. The correlation between Borealin and IQGAP1 during cytokinesis is not yet clear. Here, we used mass spectrometry and endogenous coimmunoprecipitation experiments to investigate the interaction between IQGAP1 and Borealin. Results of the current study showed that Borealin interacted directly with IQGAP1 both in vitro and in vivo. Knockdown of IQGAP1 resulted in an abnormal location of Borealin in the midbody. Knocking down Borealin alone, IQGAP1 alone, or Borealin and IQGAP1 at the same time inhibited the completion of cytokinesis and formed multinucleated cells. Our results indicated that IQGAP1 interacts with Borealin during cytokinesis, and the correct localization of Borealin in the midbody during cytokinesis is determined by IQGAP1, and IQGAP1 may play an important role in regulating Borealin function in cytokinesis. |
url |
http://dx.doi.org/10.1155/2020/6231697 |
work_keys_str_mv |
AT reziyawumaier thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT abudunaibiaili thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT hexigesaiyin thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT pingzhaozhang thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT lihuancao thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT aikeremujiangmuheremu thecorrectlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT reziyawumaier correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT abudunaibiaili correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT hexigesaiyin correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT pingzhaozhang correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT lihuancao correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 AT aikeremujiangmuheremu correctlocalizationofborealininmidbodyduringcytokinesisdependsoniqgap1 |
_version_ |
1715435701102706688 |