Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.)
The genetic control of host response to the fungal necrotrophic disease Septoria nodorum blotch (SNB) in bread wheat is complex, involving many minor genes. Quantitative trait loci (QTL) controlling SNB response were previously identified on chromosomes 1BS and 5BL. The aim of this study, therefore,...
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doaj-c116dc2a048149b8909b075d4848d28e2021-06-01T01:02:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225580558010.3390/ijms22115580Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.)Dora Li0Esther Walker1Michael Francki2State Agricultural Biotechnology Centre, Murdoch University, South St, Murdoch, WA 6150, AustraliaState Agricultural Biotechnology Centre, Murdoch University, South St, Murdoch, WA 6150, AustraliaState Agricultural Biotechnology Centre, Murdoch University, South St, Murdoch, WA 6150, AustraliaThe genetic control of host response to the fungal necrotrophic disease Septoria nodorum blotch (SNB) in bread wheat is complex, involving many minor genes. Quantitative trait loci (QTL) controlling SNB response were previously identified on chromosomes 1BS and 5BL. The aim of this study, therefore, was to align and compare the genetic map representing QTL interval on 1BS and 5BS with the reference sequence of wheat and identify resistance genes (<i>R</i>-genes) associated with SNB response. Alignment of QTL intervals identified significant genome rearrangements on 1BS between parents of the DH population EGA Blanco, Millewa and the reference sequence of Chinese Spring with subtle rearrangements on 5BL. Nevertheless, annotation of genomic intervals in the reference sequence were able to identify and map 13 and 12 <i>R</i>-genes on 1BS and 5BL, respectively. <i>R</i>-genes discriminated co-located QTL on 1BS into two distinct but linked loci. <i>NRC1a and TFIID</i> mapped in one QTL on 1BS whereas <i>RGA</i> and <i>Snn1</i> mapped in the linked locus and all were associated with SNB resistance but in one environment only. Similarly, <i>Tsn1</i> and <i>WK35</i> were mapped in one QTL on 5BL with <i>NETWORKED 1A</i> and <i>RGA</i> genes mapped in the linked QTL interval. This study provided new insights on possible biochemical, cellular and molecular mechanisms responding to SNB infection in different environments and also addressed limitations of using the reference sequence to identify the full complement of functional <i>R</i>-genes in modern varieties.https://www.mdpi.com/1422-0067/22/11/5580septoria nodorum blotch (SNB)wheatquantitative trait loci (QTL)resistanceinternational wheat genome sequencing Consortium (IWGSC) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dora Li Esther Walker Michael Francki |
spellingShingle |
Dora Li Esther Walker Michael Francki Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) International Journal of Molecular Sciences septoria nodorum blotch (SNB) wheat quantitative trait loci (QTL) resistance international wheat genome sequencing Consortium (IWGSC) |
author_facet |
Dora Li Esther Walker Michael Francki |
author_sort |
Dora Li |
title |
Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) |
title_short |
Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) |
title_full |
Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) |
title_fullStr |
Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) |
title_full_unstemmed |
Genes Associated with Foliar Resistance to Septoria Nodorum Blotch of Hexaploid Wheat (<i>Triticum aestivum</i> L.) |
title_sort |
genes associated with foliar resistance to septoria nodorum blotch of hexaploid wheat (<i>triticum aestivum</i> l.) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-05-01 |
description |
The genetic control of host response to the fungal necrotrophic disease Septoria nodorum blotch (SNB) in bread wheat is complex, involving many minor genes. Quantitative trait loci (QTL) controlling SNB response were previously identified on chromosomes 1BS and 5BL. The aim of this study, therefore, was to align and compare the genetic map representing QTL interval on 1BS and 5BS with the reference sequence of wheat and identify resistance genes (<i>R</i>-genes) associated with SNB response. Alignment of QTL intervals identified significant genome rearrangements on 1BS between parents of the DH population EGA Blanco, Millewa and the reference sequence of Chinese Spring with subtle rearrangements on 5BL. Nevertheless, annotation of genomic intervals in the reference sequence were able to identify and map 13 and 12 <i>R</i>-genes on 1BS and 5BL, respectively. <i>R</i>-genes discriminated co-located QTL on 1BS into two distinct but linked loci. <i>NRC1a and TFIID</i> mapped in one QTL on 1BS whereas <i>RGA</i> and <i>Snn1</i> mapped in the linked locus and all were associated with SNB resistance but in one environment only. Similarly, <i>Tsn1</i> and <i>WK35</i> were mapped in one QTL on 5BL with <i>NETWORKED 1A</i> and <i>RGA</i> genes mapped in the linked QTL interval. This study provided new insights on possible biochemical, cellular and molecular mechanisms responding to SNB infection in different environments and also addressed limitations of using the reference sequence to identify the full complement of functional <i>R</i>-genes in modern varieties. |
topic |
septoria nodorum blotch (SNB) wheat quantitative trait loci (QTL) resistance international wheat genome sequencing Consortium (IWGSC) |
url |
https://www.mdpi.com/1422-0067/22/11/5580 |
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