INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana.
INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae,...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019-01-01
|
Series: | PLoS Pathogens |
Online Access: | https://doi.org/10.1371/journal.ppat.1007499 |
id |
doaj-c11e4629f9e84e7eb2bb4456d0ba2c63 |
---|---|
record_format |
Article |
spelling |
doaj-c11e4629f9e84e7eb2bb4456d0ba2c632021-04-21T17:11:19ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742019-01-01151e100749910.1371/journal.ppat.1007499INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana.Ronny VölzSoon-Kap KimJianing MiAnamika A RawatAlaguraj VeluchamyKiruthiga G MariappanNaganand RayapuramJean-Michel DavierePatrick AchardIkram BlilouSalim Al-BabiliMoussa BenhamedHeribert HirtINDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of basal immune response and PAMP-triggered immunity. Furthermore, the idd4 mutant exhibits enhanced plant-growth indicating IDD4 as suppressor of growth and development. Transcriptome comparison of idd4 mutants and IDD4ox lines aligned to genome-wide IDD4 DNA-binding studies revealed major target genes related to defense and developmental-biological processes. IDD4 is a phospho-protein that interacts and becomes phosphorylated on two conserved sites by the MAP kinase MPK6. DNA-binding studies of IDD4 after flg22 treatment and with IDD4 phosphosite mutants show enhanced binding affinity to ID1 motif-containing promoters and its function as a transcriptional regulator. In contrast to the IDD4-phospho-dead mutant, the IDD4 phospho-mimicking mutant shows altered susceptibility to PstDC3000, salicylic acid levels and transcriptome reprogramming. In summary, we found that IDD4 regulates various hormonal pathways thereby coordinating growth and development with basal immunity.https://doi.org/10.1371/journal.ppat.1007499 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ronny Völz Soon-Kap Kim Jianing Mi Anamika A Rawat Alaguraj Veluchamy Kiruthiga G Mariappan Naganand Rayapuram Jean-Michel Daviere Patrick Achard Ikram Blilou Salim Al-Babili Moussa Benhamed Heribert Hirt |
spellingShingle |
Ronny Völz Soon-Kap Kim Jianing Mi Anamika A Rawat Alaguraj Veluchamy Kiruthiga G Mariappan Naganand Rayapuram Jean-Michel Daviere Patrick Achard Ikram Blilou Salim Al-Babili Moussa Benhamed Heribert Hirt INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. PLoS Pathogens |
author_facet |
Ronny Völz Soon-Kap Kim Jianing Mi Anamika A Rawat Alaguraj Veluchamy Kiruthiga G Mariappan Naganand Rayapuram Jean-Michel Daviere Patrick Achard Ikram Blilou Salim Al-Babili Moussa Benhamed Heribert Hirt |
author_sort |
Ronny Völz |
title |
INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. |
title_short |
INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. |
title_full |
INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. |
title_fullStr |
INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. |
title_full_unstemmed |
INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana. |
title_sort |
indeterminate-domain 4 (idd4) coordinates immune responses with plant-growth in arabidopsis thaliana. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2019-01-01 |
description |
INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of basal immune response and PAMP-triggered immunity. Furthermore, the idd4 mutant exhibits enhanced plant-growth indicating IDD4 as suppressor of growth and development. Transcriptome comparison of idd4 mutants and IDD4ox lines aligned to genome-wide IDD4 DNA-binding studies revealed major target genes related to defense and developmental-biological processes. IDD4 is a phospho-protein that interacts and becomes phosphorylated on two conserved sites by the MAP kinase MPK6. DNA-binding studies of IDD4 after flg22 treatment and with IDD4 phosphosite mutants show enhanced binding affinity to ID1 motif-containing promoters and its function as a transcriptional regulator. In contrast to the IDD4-phospho-dead mutant, the IDD4 phospho-mimicking mutant shows altered susceptibility to PstDC3000, salicylic acid levels and transcriptome reprogramming. In summary, we found that IDD4 regulates various hormonal pathways thereby coordinating growth and development with basal immunity. |
url |
https://doi.org/10.1371/journal.ppat.1007499 |
work_keys_str_mv |
AT ronnyvolz indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT soonkapkim indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT jianingmi indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT anamikaarawat indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT alagurajveluchamy indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT kiruthigagmariappan indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT naganandrayapuram indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT jeanmicheldaviere indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT patrickachard indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT ikramblilou indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT salimalbabili indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT moussabenhamed indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana AT heriberthirt indeterminatedomain4idd4coordinatesimmuneresponseswithplantgrowthinarabidopsisthaliana |
_version_ |
1714666533584633856 |