Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.)
High temperature at anthesis is one of the most serious stress factors for rice (<i>Oryza sativa</i> L.) production, causing irreversible yield losses and reduces grain quality. Illustration of thermotolerance mechanism is of great importance to accelerate rice breeding aimed at thermoto...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-02-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/3/1155 |
id |
doaj-c137a46c595149e9a4538f1b46a9f394 |
---|---|
record_format |
Article |
spelling |
doaj-c137a46c595149e9a4538f1b46a9f3942020-11-25T02:03:34ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-02-01213115510.3390/ijms21031155ijms21031155Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.)Gang Liu0Zhongping Zha1Haiya Cai2Dandan Qin3Haitao Jia4Changyan Liu5Dongfeng Qiu6Zaijun Zhang7Zhenghuang Wan8Yuanyuan Yang9Bingliang Wan10Aiqing You11Chunhai Jiao12Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaHigh temperature at anthesis is one of the most serious stress factors for rice (<i>Oryza sativa</i> L.) production, causing irreversible yield losses and reduces grain quality. Illustration of thermotolerance mechanism is of great importance to accelerate rice breeding aimed at thermotolerance improvement. Here, we identified a new thermotolerant germplasm, SDWG005. Microscopical analysis found that stable anther structure of SDWG005 under stress may contribute to its thermotolerance. Dynamic transcriptomic analysis totally identified 3559 differentially expressed genes (DEGs) in SDWG005 anthers at anthesis under heat treatments, including 477, 869, 2335, and 2210 for 1, 2, 6, and 12 h, respectively; however, only 131 were regulated across all four-time-points. The DEGs were divided into nine clusters according to their expressions in these heat treatments. Further analysis indicated that some main gene categories involved in heat-response of SDWG005 anthers, such as transcription factors, nucleic acid and protein metabolisms related genes, etc. Comparison with previous studies indicates that a core gene-set may exist for thermotolerance mechanism. Expression and polymorphic analysis of agmatine-coumarin-acyltransferase gene <i>OsACT</i> in different accessions suggested that it may involve in SDWG005 thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis, and also lays foundation for breeding thermotolerant varieties via molecular breeding.https://www.mdpi.com/1422-0067/21/3/1155riceheat stresstranscriptomeantheranthesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gang Liu Zhongping Zha Haiya Cai Dandan Qin Haitao Jia Changyan Liu Dongfeng Qiu Zaijun Zhang Zhenghuang Wan Yuanyuan Yang Bingliang Wan Aiqing You Chunhai Jiao |
spellingShingle |
Gang Liu Zhongping Zha Haiya Cai Dandan Qin Haitao Jia Changyan Liu Dongfeng Qiu Zaijun Zhang Zhenghuang Wan Yuanyuan Yang Bingliang Wan Aiqing You Chunhai Jiao Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) International Journal of Molecular Sciences rice heat stress transcriptome anther anthesis |
author_facet |
Gang Liu Zhongping Zha Haiya Cai Dandan Qin Haitao Jia Changyan Liu Dongfeng Qiu Zaijun Zhang Zhenghuang Wan Yuanyuan Yang Bingliang Wan Aiqing You Chunhai Jiao |
author_sort |
Gang Liu |
title |
Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) |
title_short |
Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) |
title_full |
Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) |
title_fullStr |
Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) |
title_full_unstemmed |
Dynamic Transcriptome Analysis of Anther Response to Heat Stress during Anthesis in Thermotolerant Rice (<i>Oryza sativa</i> L.) |
title_sort |
dynamic transcriptome analysis of anther response to heat stress during anthesis in thermotolerant rice (<i>oryza sativa</i> l.) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-02-01 |
description |
High temperature at anthesis is one of the most serious stress factors for rice (<i>Oryza sativa</i> L.) production, causing irreversible yield losses and reduces grain quality. Illustration of thermotolerance mechanism is of great importance to accelerate rice breeding aimed at thermotolerance improvement. Here, we identified a new thermotolerant germplasm, SDWG005. Microscopical analysis found that stable anther structure of SDWG005 under stress may contribute to its thermotolerance. Dynamic transcriptomic analysis totally identified 3559 differentially expressed genes (DEGs) in SDWG005 anthers at anthesis under heat treatments, including 477, 869, 2335, and 2210 for 1, 2, 6, and 12 h, respectively; however, only 131 were regulated across all four-time-points. The DEGs were divided into nine clusters according to their expressions in these heat treatments. Further analysis indicated that some main gene categories involved in heat-response of SDWG005 anthers, such as transcription factors, nucleic acid and protein metabolisms related genes, etc. Comparison with previous studies indicates that a core gene-set may exist for thermotolerance mechanism. Expression and polymorphic analysis of agmatine-coumarin-acyltransferase gene <i>OsACT</i> in different accessions suggested that it may involve in SDWG005 thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis, and also lays foundation for breeding thermotolerant varieties via molecular breeding. |
topic |
rice heat stress transcriptome anther anthesis |
url |
https://www.mdpi.com/1422-0067/21/3/1155 |
work_keys_str_mv |
AT gangliu dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT zhongpingzha dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT haiyacai dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT dandanqin dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT haitaojia dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT changyanliu dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT dongfengqiu dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT zaijunzhang dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT zhenghuangwan dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT yuanyuanyang dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT bingliangwan dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT aiqingyou dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail AT chunhaijiao dynamictranscriptomeanalysisofantherresponsetoheatstressduringanthesisinthermotolerantriceioryzasativail |
_version_ |
1724947342809890816 |