The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux
Because of its outstanding thermochromic characteristics and metal-insulator transition (MIT) property, nano-vanadium dioxide (abbreviated as nano-VO2 or nVO2) has been applied widely in electrical/optical devices and design of intelligent window. However, the biological effect of nVO2 is not well u...
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doaj-c13a6856a8ed4e0099d9ac841522d34e2020-11-24T23:15:54ZengElsevierToxicology Reports2214-75002015-01-012C87087910.1016/j.toxrep.2015.06.005The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ effluxShaohai Xu0Shengmin Xu1Shaopeng Chen2Huadong Fan3Xun Luo4Yuxiang Sun5Jun Wang6Hang Yuan7An Xu8Lijun Wu9School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaSchool of Nuclear Science and Technology, University of Science and Technology of China, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaKey Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences and Anhui province, Hefei, Anhui, PR ChinaSchool of Nuclear Science and Technology, University of Science and Technology of China, Hefei, Anhui, PR ChinaBecause of its outstanding thermochromic characteristics and metal-insulator transition (MIT) property, nano-vanadium dioxide (abbreviated as nano-VO2 or nVO2) has been applied widely in electrical/optical devices and design of intelligent window. However, the biological effect of nVO2 is not well understood, especially when affected by environmental factors or living organisms. For VO2 is an amphoteric oxide, we simulated pH's influence to nVO2’s physicochemical properties by exposure nVO2 in water of different pH values. We found that nVO2 transformed to a new product after exposure in acidic water for two weeks, as revealed by physicochemical characterization such as SEM, TEM, XRD, and DLS. This transformation product formed in acidic water was referred as (acidic) transformed nVO2). Both pristine/untransformed and transformed nVO2 displayed no obvious toxicity to common epithelial cells; however, the acidic transformed nVO2 rapidly induced macrophage cell death. Further investigation demonstrated that transformed nVO2 caused macrophage apoptosis by the induction of Ca2+ efflux and the following mitochondrial membrane permeabilization (MMP) process. And a more detailed time course study indicated that transformed nVO2 caused lysosomal membrane permeabilization (LMP) at the earlier stage, indicating LMP could be chosen as an earlier and sensitive end point for nanotoxicological study. We conclude that although nVO2 displays no acute toxicity, its acidic transformation product induces macrophage apoptosis by the induction of LMP and Ca2+ efflux. This report suggests that the interplay with environmental factors or living organisms can results in physicochemical transformation of nanomaterials and the ensuing distinctive biological effects.http://www.sciencedirect.com/science/article/pii/S2214750015300147Nanoparticle transformationMacrophage cell deathLysosomal membrane permeabilizationCa2+ efflux |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shaohai Xu Shengmin Xu Shaopeng Chen Huadong Fan Xun Luo Yuxiang Sun Jun Wang Hang Yuan An Xu Lijun Wu |
spellingShingle |
Shaohai Xu Shengmin Xu Shaopeng Chen Huadong Fan Xun Luo Yuxiang Sun Jun Wang Hang Yuan An Xu Lijun Wu The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux Toxicology Reports Nanoparticle transformation Macrophage cell death Lysosomal membrane permeabilization Ca2+ efflux |
author_facet |
Shaohai Xu Shengmin Xu Shaopeng Chen Huadong Fan Xun Luo Yuxiang Sun Jun Wang Hang Yuan An Xu Lijun Wu |
author_sort |
Shaohai Xu |
title |
The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux |
title_short |
The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux |
title_full |
The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux |
title_fullStr |
The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux |
title_full_unstemmed |
The acidic transformed nano-VO2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and Ca2+ efflux |
title_sort |
acidic transformed nano-vo2 causes macrophage cell death by the induction of lysosomal membrane permeabilization and ca2+ efflux |
publisher |
Elsevier |
series |
Toxicology Reports |
issn |
2214-7500 |
publishDate |
2015-01-01 |
description |
Because of its outstanding thermochromic characteristics and metal-insulator transition (MIT) property, nano-vanadium dioxide (abbreviated as nano-VO2 or nVO2) has been applied widely in electrical/optical devices and design of intelligent window. However, the biological effect of nVO2 is not well understood, especially when affected by environmental factors or living organisms. For VO2 is an amphoteric oxide, we simulated pH's influence to nVO2’s physicochemical properties by exposure nVO2 in water of different pH values. We found that nVO2 transformed to a new product after exposure in acidic water for two weeks, as revealed by physicochemical characterization such as SEM, TEM, XRD, and DLS. This transformation product formed in acidic water was referred as (acidic) transformed nVO2). Both pristine/untransformed and transformed nVO2 displayed no obvious toxicity to common epithelial cells; however, the acidic transformed nVO2 rapidly induced macrophage cell death. Further investigation demonstrated that transformed nVO2 caused macrophage apoptosis by the induction of Ca2+ efflux and the following mitochondrial membrane permeabilization (MMP) process. And a more detailed time course study indicated that transformed nVO2 caused lysosomal membrane permeabilization (LMP) at the earlier stage, indicating LMP could be chosen as an earlier and sensitive end point for nanotoxicological study. We conclude that although nVO2 displays no acute toxicity, its acidic transformation product induces macrophage apoptosis by the induction of LMP and Ca2+ efflux. This report suggests that the interplay with environmental factors or living organisms can results in physicochemical transformation of nanomaterials and the ensuing distinctive biological effects. |
topic |
Nanoparticle transformation Macrophage cell death Lysosomal membrane permeabilization Ca2+ efflux |
url |
http://www.sciencedirect.com/science/article/pii/S2214750015300147 |
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