Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation
Mounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main do...
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doaj-c13df7e4f30e40d08ddb7f13d55253b82021-04-30T23:05:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01224790479010.3390/ijms22094790Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen StarvationRomina Beleggia0Nooshin Omranian1Yan Holtz2Tania Gioia3Fabio Fiorani4Franca M. Nigro5Nicola Pecchioni6Pasquale De Vita7Ulrich Schurr8Jacques L. David9Zoran Nikoloski10Roberto Papa11Council for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, ItalySystems Biology and Mathematical Modeling Group, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, GermanyMontpellier SupAgro, UMR Amelioration Genetique et Adaptation des Plantes, 34060 Montpellier, FranceScuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, 85100 Potenza, ItalyInstitute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Julich GmbH, 52428 Julich, GermanyCouncil for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, ItalyCouncil for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, ItalyCouncil for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, ItalyInstitute of Biosciences and Geosciences (IBG-2): Plant Sciences, Forschungszentrum Julich GmbH, 52428 Julich, GermanyMontpellier SupAgro, UMR Amelioration Genetique et Adaptation des Plantes, 34060 Montpellier, FranceSystems Biology and Mathematical Modeling Group, Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam, GermanyCouncil for Agricultural Research and Economics (CREA), Research Centre for Cereal and Industrial Crops (CREA-CI), 71122 Foggia, ItalyMounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main domesticated subspecies: emmer and durum wheat. We found that durum wheat exhibits broader and stronger response in comparison to emmer as seen from the expression pattern of both genes and metabolites and gene enrichment analysis. They showed major differences in the responses to N starvation for transcription factor families, emmer showed differential reduction in the levels of primary metabolites while durum wheat exhibited increased levels of most of them to N starvation. The correlation-based networks, including the differentially expressed genes and metabolites, revealed tighter regulation of metabolism in durum wheat in comparison to emmer. We also found that glutamate and -aminobutyric acid (GABA) had highest values of centrality in the metabolic correlation network, suggesting their critical role in the genotype-specific response to N starvation of emmer and durum wheat, respectively. Moreover, this finding indicates that there might be contrasting strategies associated to GABA and glutamate signaling modulating shoot vs. root growth in the two different wheat subspecies.https://www.mdpi.com/1422-0067/22/9/4790plant nutrition<i>Triticum turgidum</i>transcriptomicsmetabolomicsstressGABA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Romina Beleggia Nooshin Omranian Yan Holtz Tania Gioia Fabio Fiorani Franca M. Nigro Nicola Pecchioni Pasquale De Vita Ulrich Schurr Jacques L. David Zoran Nikoloski Roberto Papa |
spellingShingle |
Romina Beleggia Nooshin Omranian Yan Holtz Tania Gioia Fabio Fiorani Franca M. Nigro Nicola Pecchioni Pasquale De Vita Ulrich Schurr Jacques L. David Zoran Nikoloski Roberto Papa Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation International Journal of Molecular Sciences plant nutrition <i>Triticum turgidum</i> transcriptomics metabolomics stress GABA |
author_facet |
Romina Beleggia Nooshin Omranian Yan Holtz Tania Gioia Fabio Fiorani Franca M. Nigro Nicola Pecchioni Pasquale De Vita Ulrich Schurr Jacques L. David Zoran Nikoloski Roberto Papa |
author_sort |
Romina Beleggia |
title |
Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation |
title_short |
Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation |
title_full |
Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation |
title_fullStr |
Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation |
title_full_unstemmed |
Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation |
title_sort |
comparative analysis based on transcriptomics and metabolomics data reveal differences between emmer and durum wheat in response to nitrogen starvation |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-04-01 |
description |
Mounting evidence indicates the key role of nitrogen (N) on diverse processes in plant, including development and defense. Using a combined transcriptomics and metabolomics approach, we studied the response of seedlings to N starvation of two different tetraploid wheat genotypes from the two main domesticated subspecies: emmer and durum wheat. We found that durum wheat exhibits broader and stronger response in comparison to emmer as seen from the expression pattern of both genes and metabolites and gene enrichment analysis. They showed major differences in the responses to N starvation for transcription factor families, emmer showed differential reduction in the levels of primary metabolites while durum wheat exhibited increased levels of most of them to N starvation. The correlation-based networks, including the differentially expressed genes and metabolites, revealed tighter regulation of metabolism in durum wheat in comparison to emmer. We also found that glutamate and -aminobutyric acid (GABA) had highest values of centrality in the metabolic correlation network, suggesting their critical role in the genotype-specific response to N starvation of emmer and durum wheat, respectively. Moreover, this finding indicates that there might be contrasting strategies associated to GABA and glutamate signaling modulating shoot vs. root growth in the two different wheat subspecies. |
topic |
plant nutrition <i>Triticum turgidum</i> transcriptomics metabolomics stress GABA |
url |
https://www.mdpi.com/1422-0067/22/9/4790 |
work_keys_str_mv |
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