Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice
Multidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isola...
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doaj-c14d67c8e4eb4d82aca8996cde7ec4302020-11-24T23:18:57ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/752930752930Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in MiceFang Cao0Xitao Wang1Linhui Wang2Zhen Li3Jian Che4Lili Wang5Xiaoyu Li6Zhenhui Cao7Jiancheng Zhang8Liji Jin9Yongping Xu10School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaSchool of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, ChinaMultidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isolate KP 1513 as the host and was characterized. It produced a clear plaque with a halo and was classified as Siphoviridae. It had a short latent period of 30 min, a burst size of 264 and could inhibit KP 1513 growth in vitro with a dose-dependent pattern. Intranasal administration of a single dose of 2 × 109 PFU/mouse 2 h after KP 1513 inoculation was able to protect mice against lethal pneumonia. In a sublethal pneumonia model, phage-treated mice exhibited a lower level of K. pneumoniae burden in the lungs as compared to the untreated control. These mice lost less body weight and exhibited lower levels of inflammatory cytokines in their lungs. Lung lesion conditions were obviously improved by phage therapy. Therefore, phage 1513 has a great effect in vitro and in vivo, which has potential to be used as an alternative to an antibiotic treatment of pneumonia that is caused by the multidrug-resistant K. pneumoniae.http://dx.doi.org/10.1155/2015/752930 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang Cao Xitao Wang Linhui Wang Zhen Li Jian Che Lili Wang Xiaoyu Li Zhenhui Cao Jiancheng Zhang Liji Jin Yongping Xu |
spellingShingle |
Fang Cao Xitao Wang Linhui Wang Zhen Li Jian Che Lili Wang Xiaoyu Li Zhenhui Cao Jiancheng Zhang Liji Jin Yongping Xu Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice BioMed Research International |
author_facet |
Fang Cao Xitao Wang Linhui Wang Zhen Li Jian Che Lili Wang Xiaoyu Li Zhenhui Cao Jiancheng Zhang Liji Jin Yongping Xu |
author_sort |
Fang Cao |
title |
Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice |
title_short |
Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice |
title_full |
Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice |
title_fullStr |
Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice |
title_full_unstemmed |
Evaluation of the Efficacy of a Bacteriophage in the Treatment of Pneumonia Induced by Multidrug Resistance Klebsiella pneumoniae in Mice |
title_sort |
evaluation of the efficacy of a bacteriophage in the treatment of pneumonia induced by multidrug resistance klebsiella pneumoniae in mice |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Multidrug-resistant Klebsiella pneumoniae (MRKP) has steadily grown beyond antibiotic control. However, a bacteriophage is considered to be a potential antibiotic alternative for treating bacterial infections. In this study, a lytic bacteriophage, phage 1513, was isolated using a clinical MRKP isolate KP 1513 as the host and was characterized. It produced a clear plaque with a halo and was classified as Siphoviridae. It had a short latent period of 30 min, a burst size of 264 and could inhibit KP 1513 growth in vitro with a dose-dependent pattern. Intranasal administration of a single dose of 2 × 109 PFU/mouse 2 h after KP 1513 inoculation was able to protect mice against lethal pneumonia. In a sublethal pneumonia model, phage-treated mice exhibited a lower level of K. pneumoniae burden in the lungs as compared to the untreated control. These mice lost less body weight and exhibited lower levels of inflammatory cytokines in their lungs. Lung lesion conditions were obviously improved by phage therapy. Therefore, phage 1513 has a great effect in vitro and in vivo, which has potential to be used as an alternative to an antibiotic treatment of pneumonia that is caused by the multidrug-resistant K. pneumoniae. |
url |
http://dx.doi.org/10.1155/2015/752930 |
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