EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p
Abstract Background Glioma is one prevalent malignant tumor originates from the central nervous system. Dysregulation of long non-coding RNAs (lncRNAs) has been found to be a molecular signature behind the pathology of a variety of cancers, including glioma. EIF3J antisense RNA 1 (EIF3J-AS1) is a no...
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doaj-c152512e6559456dba4eb5bb5767de442020-11-25T02:53:11ZengBMCCancer Cell International1475-28672020-09-0120111310.1186/s12935-020-01487-2EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3pJianguo Qi0Zhengrui Wang1Zhensheng Zhao2Lijun Liu3Department of Neurosuigery, The Third People’s Hospital of JinanDepartment of Neurosurgery, Chengyang People’s Hospital of QingdaoDepartment of Hyperbaric Oxygen Therapy, Yidu Central Hospital of WeifangDepartment of Neurosurgery, Xiangyang No.1 People’s Hospital Affiliated to Hubei University of MedicineAbstract Background Glioma is one prevalent malignant tumor originates from the central nervous system. Dysregulation of long non-coding RNAs (lncRNAs) has been found to be a molecular signature behind the pathology of a variety of cancers, including glioma. EIF3J antisense RNA 1 (EIF3J-AS1) is a novel lncRNA, whose performance in carcinogenesis has been unfolded. Nevertheless, the role of EIF3J-AS1 has never been investigated in glioma. Methods qRT-PCR analysis was adopted to evaluate the relative levels of RNAs. In vitro functional assays, including colony formation, EdU, TUNEL and caspase-3/8/9 activity assays were conducted to study the impacts of EIF3J-AS1 on glioma. Dual-luciferase activity assays, RNA pull down assay and RIP assay were performed to elucidate molecular interplay among genes. Results EIF3J-AS1 was overexpressed in glioma cell lines. Knockdown of EIF3J-AS1 hampered glioma malignant phenotypes. MiR-1343-3p could bind to EIF3J-AS1. Moreover, miR-1343-3p targeted Annexin A11 (ANXA11) in its 3′UTR region. Mechanistically, EIF3J-AS1 relieved ANXA11 from miR-1343-3p silencing in the EIF3J-AS1/miR-1343-3p/ANXA11 RNA induced silencing complex (RISC), thus eliciting promoting effects on glioma progression. MiR-1343-3p inhibitor and ANXA11 overexpression offset the inhibitory impacts of EIF3J-AS1 silencing on glioma development. Conclusion EIF3J-AS1/miR-1343-3p/ANXA11 axis significantly affected biological behaviors in glioma, suggesting new therapeutic target for glioma treatment.http://link.springer.com/article/10.1186/s12935-020-01487-2GliomaEIF3J-AS1miR-1343-3pANXA11 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jianguo Qi Zhengrui Wang Zhensheng Zhao Lijun Liu |
spellingShingle |
Jianguo Qi Zhengrui Wang Zhensheng Zhao Lijun Liu EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p Cancer Cell International Glioma EIF3J-AS1 miR-1343-3p ANXA11 |
author_facet |
Jianguo Qi Zhengrui Wang Zhensheng Zhao Lijun Liu |
author_sort |
Jianguo Qi |
title |
EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p |
title_short |
EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p |
title_full |
EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p |
title_fullStr |
EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p |
title_full_unstemmed |
EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p |
title_sort |
eif3j-as1 promotes glioma cell growth via up-regulating anxa11 through sponging mir-1343-3p |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2020-09-01 |
description |
Abstract Background Glioma is one prevalent malignant tumor originates from the central nervous system. Dysregulation of long non-coding RNAs (lncRNAs) has been found to be a molecular signature behind the pathology of a variety of cancers, including glioma. EIF3J antisense RNA 1 (EIF3J-AS1) is a novel lncRNA, whose performance in carcinogenesis has been unfolded. Nevertheless, the role of EIF3J-AS1 has never been investigated in glioma. Methods qRT-PCR analysis was adopted to evaluate the relative levels of RNAs. In vitro functional assays, including colony formation, EdU, TUNEL and caspase-3/8/9 activity assays were conducted to study the impacts of EIF3J-AS1 on glioma. Dual-luciferase activity assays, RNA pull down assay and RIP assay were performed to elucidate molecular interplay among genes. Results EIF3J-AS1 was overexpressed in glioma cell lines. Knockdown of EIF3J-AS1 hampered glioma malignant phenotypes. MiR-1343-3p could bind to EIF3J-AS1. Moreover, miR-1343-3p targeted Annexin A11 (ANXA11) in its 3′UTR region. Mechanistically, EIF3J-AS1 relieved ANXA11 from miR-1343-3p silencing in the EIF3J-AS1/miR-1343-3p/ANXA11 RNA induced silencing complex (RISC), thus eliciting promoting effects on glioma progression. MiR-1343-3p inhibitor and ANXA11 overexpression offset the inhibitory impacts of EIF3J-AS1 silencing on glioma development. Conclusion EIF3J-AS1/miR-1343-3p/ANXA11 axis significantly affected biological behaviors in glioma, suggesting new therapeutic target for glioma treatment. |
topic |
Glioma EIF3J-AS1 miR-1343-3p ANXA11 |
url |
http://link.springer.com/article/10.1186/s12935-020-01487-2 |
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