Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2

• Main Authors: Li Y, Qiao L, Zang Y, Ni W, Xu Z
• Format: Article
• Language: English
• Published: Dove Medical Press 2020-06-01
• Series: Cancer Management and Research
• Subjects: bladder cancer; circ-foxo3; mir-9-5p; tgfbr2
• Online Access: https://www.dovepress.com/circular-rna-foxo3-suppresses-bladder-cancer-progression-and-metastasi-peer-reviewed-article-CMAR
• ISSN: 1179-1322

Yongxiang Li,1,2 Liang Qiao,2 Yuanwei Zang,1 Wenjun Ni,3 Zhonghua Xu1 1Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China; 2Department of Urology, Weifang People’s Hospital, Weifang 261000, People’s Republic of China; 3Department of Urology, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People’s Republic of ChinaCorrespondence: Zhonghua XuDepartment of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of ChinaTel [phone]Fax [phone]Email [e-mail]: Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions.Methods: In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation, migration, and invasion abilities were analyzed. We also used bioinformatics tools to predict potential-binding miRNAs for circ-FOXO3, and luciferase reporter assay was used for the verification of binding miRNAs. For the further study, we analyzed potential downstream-binding mRNA for miRNA, and cell proliferation, migration and invasion abilities of it were also studied.Results: We found that circ-FOXO3 was significantly down-regulated in bladder cancer (BC) tissues compared to normal bladder tissues. We also found that circ-FOXO3 overexpression inhibited cell proliferation, migration and invasion in BC cell lines. Moreover, we demonstrated that TGFBR2 was regulated by circ-FOXO3 through sponging miR-9-5p, the knockdown of TGFBR2 or the overexpression of miR-9-5p all related to the increased BC cell proliferation, migration, and invasion.Discussion: In summary, our data showed that circ-FOXO3 was significantly down-regulated in bladder cancers. circ-FOXO3 overexpression inhibits BC cell progression and metastasis. Furthermore, circ-FOXO3 regulates TGFBR2 expression through sponging miR-9-5p in BC cell lines.Keywords: bladder cancer, circ-FOXO3, miR-9-5p, TGFBR2