Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2
Yongxiang Li,1,2 Liang Qiao,2 Yuanwei Zang,1 Wenjun Ni,3 Zhonghua Xu1 1Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China; 2Department of Urology, Weifang People’s Hospital, Weifang 261000, People’s Republic of China; 3...
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doaj-c18a13b73e434e6590791f0b1dfa0e072020-11-25T03:49:20ZengDove Medical PressCancer Management and Research1179-13222020-06-01Volume 125049505654838Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2Li YQiao LZang YNi WXu ZYongxiang Li,1,2 Liang Qiao,2 Yuanwei Zang,1 Wenjun Ni,3 Zhonghua Xu1 1Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China; 2Department of Urology, Weifang People’s Hospital, Weifang 261000, People’s Republic of China; 3Department of Urology, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People’s Republic of ChinaCorrespondence: Zhonghua XuDepartment of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of ChinaTel +86-531-82166701Fax +86-531-86927544Email bildsc@163.comBackground: Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions.Methods: In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation, migration, and invasion abilities were analyzed. We also used bioinformatics tools to predict potential-binding miRNAs for circ-FOXO3, and luciferase reporter assay was used for the verification of binding miRNAs. For the further study, we analyzed potential downstream-binding mRNA for miRNA, and cell proliferation, migration and invasion abilities of it were also studied.Results: We found that circ-FOXO3 was significantly down-regulated in bladder cancer (BC) tissues compared to normal bladder tissues. We also found that circ-FOXO3 overexpression inhibited cell proliferation, migration and invasion in BC cell lines. Moreover, we demonstrated that TGFBR2 was regulated by circ-FOXO3 through sponging miR-9-5p, the knockdown of TGFBR2 or the overexpression of miR-9-5p all related to the increased BC cell proliferation, migration, and invasion.Discussion: In summary, our data showed that circ-FOXO3 was significantly down-regulated in bladder cancers. circ-FOXO3 overexpression inhibits BC cell progression and metastasis. Furthermore, circ-FOXO3 regulates TGFBR2 expression through sponging miR-9-5p in BC cell lines.Keywords: bladder cancer, circ-FOXO3, miR-9-5p, TGFBR2https://www.dovepress.com/circular-rna-foxo3-suppresses-bladder-cancer-progression-and-metastasi-peer-reviewed-article-CMARbladder cancercirc-foxo3mir-9-5ptgfbr2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Y Qiao L Zang Y Ni W Xu Z |
spellingShingle |
Li Y Qiao L Zang Y Ni W Xu Z Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 Cancer Management and Research bladder cancer circ-foxo3 mir-9-5p tgfbr2 |
author_facet |
Li Y Qiao L Zang Y Ni W Xu Z |
author_sort |
Li Y |
title |
Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 |
title_short |
Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 |
title_full |
Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 |
title_fullStr |
Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 |
title_full_unstemmed |
Circular RNA FOXO3 Suppresses Bladder Cancer Progression and Metastasis by Regulating MiR-9-5p/TGFBR2 |
title_sort |
circular rna foxo3 suppresses bladder cancer progression and metastasis by regulating mir-9-5p/tgfbr2 |
publisher |
Dove Medical Press |
series |
Cancer Management and Research |
issn |
1179-1322 |
publishDate |
2020-06-01 |
description |
Yongxiang Li,1,2 Liang Qiao,2 Yuanwei Zang,1 Wenjun Ni,3 Zhonghua Xu1 1Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of China; 2Department of Urology, Weifang People’s Hospital, Weifang 261000, People’s Republic of China; 3Department of Urology, Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, People’s Republic of ChinaCorrespondence: Zhonghua XuDepartment of Urology, Qilu Hospital of Shandong University, Jinan 250012, People’s Republic of ChinaTel +86-531-82166701Fax +86-531-86927544Email bildsc@163.comBackground: Increasing evidence indicates that the dysregulation of circular RNAs (circRNAs) plays important roles in tumor progressions.Methods: In this study, we first analyzed circ-FOXO3 level in bladder cancers (BCs), and then BC cell lines were transfected with circ-FOXO3 expression vector, and cell proliferation, migration, and invasion abilities were analyzed. We also used bioinformatics tools to predict potential-binding miRNAs for circ-FOXO3, and luciferase reporter assay was used for the verification of binding miRNAs. For the further study, we analyzed potential downstream-binding mRNA for miRNA, and cell proliferation, migration and invasion abilities of it were also studied.Results: We found that circ-FOXO3 was significantly down-regulated in bladder cancer (BC) tissues compared to normal bladder tissues. We also found that circ-FOXO3 overexpression inhibited cell proliferation, migration and invasion in BC cell lines. Moreover, we demonstrated that TGFBR2 was regulated by circ-FOXO3 through sponging miR-9-5p, the knockdown of TGFBR2 or the overexpression of miR-9-5p all related to the increased BC cell proliferation, migration, and invasion.Discussion: In summary, our data showed that circ-FOXO3 was significantly down-regulated in bladder cancers. circ-FOXO3 overexpression inhibits BC cell progression and metastasis. Furthermore, circ-FOXO3 regulates TGFBR2 expression through sponging miR-9-5p in BC cell lines.Keywords: bladder cancer, circ-FOXO3, miR-9-5p, TGFBR2 |
topic |
bladder cancer circ-foxo3 mir-9-5p tgfbr2 |
url |
https://www.dovepress.com/circular-rna-foxo3-suppresses-bladder-cancer-progression-and-metastasi-peer-reviewed-article-CMAR |
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