Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1
Moderate alcohol consumption increases HDL cholesterol, which is involved in reverse cholesterol transport (RCT). The aim of this study was to investigate the effect of moderate alcohol consumption on cholesterol efflux, using J774 mouse macrophages and Fu5AH cells, and on other parameters in the RC...
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Format: | Article |
Language: | English |
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Elsevier
2004-09-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520312888 |
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doaj-c1957cfd5b274580a000c96992e11bf8 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J.W.J. Beulens A. Sierksma A. van Tol N. Fournier T. van Gent J-L. Paul H.F.J. Hendriks |
spellingShingle |
J.W.J. Beulens A. Sierksma A. van Tol N. Fournier T. van Gent J-L. Paul H.F.J. Hendriks Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 Journal of Lipid Research ATP binding cassette transporter 1 reverse cholesterol transport J774 macrophages Fu5AH cells preβ-high density lipoprotein body mass index |
author_facet |
J.W.J. Beulens A. Sierksma A. van Tol N. Fournier T. van Gent J-L. Paul H.F.J. Hendriks |
author_sort |
J.W.J. Beulens |
title |
Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 |
title_short |
Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 |
title_full |
Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 |
title_fullStr |
Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 |
title_full_unstemmed |
Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 |
title_sort |
moderate alcohol consumption increases cholesterol efflux mediated by abca1 |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2004-09-01 |
description |
Moderate alcohol consumption increases HDL cholesterol, which is involved in reverse cholesterol transport (RCT). The aim of this study was to investigate the effect of moderate alcohol consumption on cholesterol efflux, using J774 mouse macrophages and Fu5AH cells, and on other parameters in the RCT pathway. Twenty-three healthy men (45–65 years) participated in a randomized, partially diet-controlled, crossover trial. They consumed four glasses of whisky (40 g of alcohol) or water daily for 17 days. After 17 days of whisky consumption, serum capacity to induce ABCA1-dependent cholesterol efflux from J774 mouse macrophages was increased by 17.5% (P = 0.027) compared with water consumption. Plasma capacity to induce cholesterol efflux from Fu5AH cells increased by 4.6% (P = 0.002). Preβ-HDL, apolipoprotein A-I (apoA-I), and lipoprotein A-I:A-II also increased by 31.6, 6.2, and 5.7% (P < 0.05), respectively, after whisky consumption compared with water consumption. Changes of cAMP-stimulated cholesterol efflux correlated (r = 0.65, P < 0.05) with changes of apoA-I but not with changes of preβ-HDL (r = 0.30, P = 0.18). Cholesterol efflux capacities from serum of lean men were higher than those from overweight men.In conclusion, this study shows that moderate alcohol consumption increases the capacity of serum to induce cholesterol efflux from J774 mouse macrophages, which may be mediated by ABCA1. |
topic |
ATP binding cassette transporter 1 reverse cholesterol transport J774 macrophages Fu5AH cells preβ-high density lipoprotein body mass index |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520312888 |
work_keys_str_mv |
AT jwjbeulens moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT asierksma moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT avantol moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT nfournier moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT tvangent moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT jlpaul moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 AT hfjhendriks moderatealcoholconsumptionincreasescholesteroleffluxmediatedbyabca1 |
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spelling |
doaj-c1957cfd5b274580a000c96992e11bf82021-04-27T04:39:35ZengElsevierJournal of Lipid Research0022-22752004-09-0145917161723Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1J.W.J. Beulens0A. Sierksma1A. van Tol2N. Fournier3T. van Gent4J-L. Paul5H.F.J. Hendriks6Netherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceNetherlands Organization for Applied Scientific Research (TNO), Nutrition and Food Research, Zeist, The Netherlands; Wageningen University, Wageningen, The Netherlands; Departments of Biochemistry and Cell Biology & Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands; Laboratoire de Biochimie, Faculté des Sciences Pharmaceutiques, Châtenay-Malabry, FranceModerate alcohol consumption increases HDL cholesterol, which is involved in reverse cholesterol transport (RCT). The aim of this study was to investigate the effect of moderate alcohol consumption on cholesterol efflux, using J774 mouse macrophages and Fu5AH cells, and on other parameters in the RCT pathway. Twenty-three healthy men (45–65 years) participated in a randomized, partially diet-controlled, crossover trial. They consumed four glasses of whisky (40 g of alcohol) or water daily for 17 days. After 17 days of whisky consumption, serum capacity to induce ABCA1-dependent cholesterol efflux from J774 mouse macrophages was increased by 17.5% (P = 0.027) compared with water consumption. Plasma capacity to induce cholesterol efflux from Fu5AH cells increased by 4.6% (P = 0.002). Preβ-HDL, apolipoprotein A-I (apoA-I), and lipoprotein A-I:A-II also increased by 31.6, 6.2, and 5.7% (P < 0.05), respectively, after whisky consumption compared with water consumption. Changes of cAMP-stimulated cholesterol efflux correlated (r = 0.65, P < 0.05) with changes of apoA-I but not with changes of preβ-HDL (r = 0.30, P = 0.18). Cholesterol efflux capacities from serum of lean men were higher than those from overweight men.In conclusion, this study shows that moderate alcohol consumption increases the capacity of serum to induce cholesterol efflux from J774 mouse macrophages, which may be mediated by ABCA1.http://www.sciencedirect.com/science/article/pii/S0022227520312888ATP binding cassette transporter 1reverse cholesterol transportJ774 macrophagesFu5AH cellspreβ-high density lipoproteinbody mass index |