Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Ca...
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Instituto Oswaldo Cruz, Ministério da Saúde
1998-01-01
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doaj-c1b11037c4e34f268f88bd72ca1061522020-11-25T01:59:18ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80601998-01-019330330710.1590/S0074-02761998000700060Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopyJosé Roberto Machado-SilvaMarcelo Pelajo-MachadoHenrique Leonel LenziDelir Corrêa GomesAiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700060Schistosoma mansoniadult malemorphologyconfocal laser scanning microscopyreproductive system |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
José Roberto Machado-Silva Marcelo Pelajo-Machado Henrique Leonel Lenzi Delir Corrêa Gomes |
spellingShingle |
José Roberto Machado-Silva Marcelo Pelajo-Machado Henrique Leonel Lenzi Delir Corrêa Gomes Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy Memórias do Instituto Oswaldo Cruz. Schistosoma mansoni adult male morphology confocal laser scanning microscopy reproductive system |
author_facet |
José Roberto Machado-Silva Marcelo Pelajo-Machado Henrique Leonel Lenzi Delir Corrêa Gomes |
author_sort |
José Roberto Machado-Silva |
title |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_short |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_full |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_fullStr |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_full_unstemmed |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_sort |
morphological study of adult male worms of schistosoma mansoni sambon, 1907 by confocal laser scanning microscopy |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
1998-01-01 |
description |
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures. |
topic |
Schistosoma mansoni adult male morphology confocal laser scanning microscopy reproductive system |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700060 |
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