Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed
Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a devastating disease in rapeseed. The objective of this study was to investigate the role and the mechanism of silicon (Si) in alleviating the disease severity of S. sclerotiorum in rapeseed. In the absorption assays, the rapeseed that ab...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-09-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.721436/full |
id |
doaj-c1be22b74636477594c89dfcd75eba26 |
---|---|
record_format |
Article |
spelling |
doaj-c1be22b74636477594c89dfcd75eba262021-09-13T16:26:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.721436721436Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in RapeseedYuxia Feng0Yuxia Feng1Yuxia Feng2Yuxia Feng3Yuxin Hu4Pengpeng Fang5Pengpeng Fang6Pengpeng Fang7Xiangjun Zuo8Xiangjun Zuo9Xiangjun Zuo10Jinxiong Wang11Jiana Li12Jiana Li13Jiana Li14Wei Qian15Wei Qian16Wei Qian17Jiaqin Mei18Jiaqin Mei19Jiaqin Mei20College of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaSichuan Shuangliu Art and Sports Middle School, Chengdu, ChinaHanhong College, Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaInstitute of Agricultural Research, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing, ChinaAcademy of Agricultural Sciences, Southwest University, Chongqing, ChinaState Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, ChinaSclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a devastating disease in rapeseed. The objective of this study was to investigate the role and the mechanism of silicon (Si) in alleviating the disease severity of S. sclerotiorum in rapeseed. In the absorption assays, the rapeseed that absorbed 10 mM of K2SiO3 exhibited an 86% decrease in lesion size on infected leaves as compared with controls. In the spray assay, the lesion length on rapeseed stems was reduced by 30.5–32.9% with the use of 100 mM of a foliar Si fertilizer as compared with controls. In the pot assay, the lesion length on rapeseed stems was reduced by 34.9–38.3% when using the Si fertilizer as basal fertilizer. In the field assay, both the disease incidence and disease index of sclerotinia stem rot were significantly reduced with the usage of a solid Si fertilizer, Si foliar fertilizer, and the application of both, without negative affection on the main agronomic traits and seed quality of rapeseed. The transcriptome sequencing, quantitative reverse transcription PCR (qRT-PCR), and biochemical assays between K2SO4- and K2SiO3- treated rapeseed leaves revealed that Si promoted the biosynthesis of defense-related substances and enhanced the antioxidation and detoxification abilities of rapeseed after infection. Thus, this study concluded that Si can alleviate the disease severity of S. sclerotiorum in rapeseeds, partially due to the induced defense responses.https://www.frontiersin.org/articles/10.3389/fpls.2021.721436/fullSclerotinia sclerotiorumrapeseedinduced resistancedisease controlsilicon |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuxia Feng Yuxia Feng Yuxia Feng Yuxia Feng Yuxin Hu Pengpeng Fang Pengpeng Fang Pengpeng Fang Xiangjun Zuo Xiangjun Zuo Xiangjun Zuo Jinxiong Wang Jiana Li Jiana Li Jiana Li Wei Qian Wei Qian Wei Qian Jiaqin Mei Jiaqin Mei Jiaqin Mei |
spellingShingle |
Yuxia Feng Yuxia Feng Yuxia Feng Yuxia Feng Yuxin Hu Pengpeng Fang Pengpeng Fang Pengpeng Fang Xiangjun Zuo Xiangjun Zuo Xiangjun Zuo Jinxiong Wang Jiana Li Jiana Li Jiana Li Wei Qian Wei Qian Wei Qian Jiaqin Mei Jiaqin Mei Jiaqin Mei Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed Frontiers in Plant Science Sclerotinia sclerotiorum rapeseed induced resistance disease control silicon |
author_facet |
Yuxia Feng Yuxia Feng Yuxia Feng Yuxia Feng Yuxin Hu Pengpeng Fang Pengpeng Fang Pengpeng Fang Xiangjun Zuo Xiangjun Zuo Xiangjun Zuo Jinxiong Wang Jiana Li Jiana Li Jiana Li Wei Qian Wei Qian Wei Qian Jiaqin Mei Jiaqin Mei Jiaqin Mei |
author_sort |
Yuxia Feng |
title |
Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed |
title_short |
Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed |
title_full |
Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed |
title_fullStr |
Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed |
title_full_unstemmed |
Silicon Alleviates the Disease Severity of Sclerotinia Stem Rot in Rapeseed |
title_sort |
silicon alleviates the disease severity of sclerotinia stem rot in rapeseed |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-09-01 |
description |
Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a devastating disease in rapeseed. The objective of this study was to investigate the role and the mechanism of silicon (Si) in alleviating the disease severity of S. sclerotiorum in rapeseed. In the absorption assays, the rapeseed that absorbed 10 mM of K2SiO3 exhibited an 86% decrease in lesion size on infected leaves as compared with controls. In the spray assay, the lesion length on rapeseed stems was reduced by 30.5–32.9% with the use of 100 mM of a foliar Si fertilizer as compared with controls. In the pot assay, the lesion length on rapeseed stems was reduced by 34.9–38.3% when using the Si fertilizer as basal fertilizer. In the field assay, both the disease incidence and disease index of sclerotinia stem rot were significantly reduced with the usage of a solid Si fertilizer, Si foliar fertilizer, and the application of both, without negative affection on the main agronomic traits and seed quality of rapeseed. The transcriptome sequencing, quantitative reverse transcription PCR (qRT-PCR), and biochemical assays between K2SO4- and K2SiO3- treated rapeseed leaves revealed that Si promoted the biosynthesis of defense-related substances and enhanced the antioxidation and detoxification abilities of rapeseed after infection. Thus, this study concluded that Si can alleviate the disease severity of S. sclerotiorum in rapeseeds, partially due to the induced defense responses. |
topic |
Sclerotinia sclerotiorum rapeseed induced resistance disease control silicon |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.721436/full |
work_keys_str_mv |
AT yuxiafeng siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT yuxiafeng siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT yuxiafeng siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT yuxiafeng siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT yuxinhu siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT pengpengfang siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT pengpengfang siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT pengpengfang siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT xiangjunzuo siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT xiangjunzuo siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT xiangjunzuo siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jinxiongwang siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jianali siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jianali siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jianali siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT weiqian siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT weiqian siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT weiqian siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jiaqinmei siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jiaqinmei siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed AT jiaqinmei siliconalleviatesthediseaseseverityofsclerotiniastemrotinrapeseed |
_version_ |
1717380571287519232 |