<i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>

<i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>

A methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera <i>Desulfovibr...

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Main Authors: Tamara N. Nazina, Salimat K. Bidzhieva, Denis S. Grouzdev, Diyana S. Sokolova, Tatyana P. Tourova, Sofiya N. Parshina, Alexander N. Avtukh, Andrey B. Poltaraus, Azhdar K. Talybly
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Microorganisms
Subjects:
<i>Soehngenia longivitae</i>
new species
genome analysis
polyphasic taxonomy
oilfield
Online Access:https://www.mdpi.com/2076-2607/8/12/1967
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spelling doaj-c1ca050994e94c16bb1ed5ba956af5162020-12-12T00:01:31ZengMDPI AGMicroorganisms2076-26072020-12-0181967196710.3390/microorganisms8121967<i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>Tamara N. Nazina0Salimat K. Bidzhieva1Denis S. Grouzdev2Diyana S. Sokolova3Tatyana P. Tourova4Sofiya N. Parshina5Alexander N. Avtukh6Andrey B. Poltaraus7Azhdar K. Talybly8Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaSciBear LLC, 13617 Tallinn, EstoniaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaSkryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, 142290 Pushchino, RussiaEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, RussiaInstitute of Microbiology of the National Academy of Sciences of Azerbaijan, Baku AZ1073, AzerbaijanA methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera <i>Desulfovibrio</i>, <i>Soehngenia</i>, <i>Thermovirga</i>, <i>Petrimonas</i>, <i>Methanosarcina</i>, and <i>Methanomethylovorans</i>. A novel gram-positive, rod-shaped, anaerobic fermentative bacterium, strain 1933P<sup>T</sup>, was isolated from this enrichment and characterized. The strain grew at 13–55 °C (optimum 35 °C), with 0–3.0% (<i>w</i>/<i>v</i>) NaCl (optimum 0–2.0%) and in the pH range of 6.7–8.0 (optimum pH 7.0). The 16S rRNA gene sequence similarity, the average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values between strain 1933P<sup>T</sup> and the type strain of the most closely related species <i>Soehngenia saccharolytica</i> DSM 12858<sup>T</sup> were 98.5%, 70.5%, and 22.6%, respectively, and were below the threshold accepted for species demarcation. Genome-based phylogenomic analysis and physiological and biochemical characterization of the strain 1933P<sup>T</sup> (VKM B-3382<sup>T</sup> = KCTC 15984<sup>T</sup>) confirmed its affiliation to a novel species of the genus <i>Soehngenia</i>, for which the name <i>Soehngenia longivitae</i> sp. nov. is proposed. Genome analysis suggests that the new strain has potential in the degradation of proteinaceous components.https://www.mdpi.com/2076-2607/8/12/1967<i>Soehngenia longivitae</i>new speciesgenome analysispolyphasic taxonomyoilfield
collection DOAJ
language English
format Article
sources DOAJ
author Tamara N. Nazina
Salimat K. Bidzhieva
Denis S. Grouzdev
Diyana S. Sokolova
Tatyana P. Tourova
Sofiya N. Parshina
Alexander N. Avtukh
Andrey B. Poltaraus
Azhdar K. Talybly
spellingShingle Tamara N. Nazina
Salimat K. Bidzhieva
Denis S. Grouzdev
Diyana S. Sokolova
Tatyana P. Tourova
Sofiya N. Parshina
Alexander N. Avtukh
Andrey B. Poltaraus
Azhdar K. Talybly
<i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
Microorganisms
<i>Soehngenia longivitae</i>
new species
genome analysis
polyphasic taxonomy
oilfield
author_facet Tamara N. Nazina
Salimat K. Bidzhieva
Denis S. Grouzdev
Diyana S. Sokolova
Tatyana P. Tourova
Sofiya N. Parshina
Alexander N. Avtukh
Andrey B. Poltaraus
Azhdar K. Talybly
author_sort Tamara N. Nazina
title <i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
title_short <i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
title_full <i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
title_fullStr <i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
title_full_unstemmed <i>Soehngenia longivitae</i> sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus <i>Soehngenia</i>
title_sort <i>soehngenia longivitae</i> sp. nov., a fermenting bacterium isolated from a petroleum reservoir in azerbaijan, and emended description of the genus <i>soehngenia</i>
publisher MDPI AG
series Microorganisms
issn 2076-2607
publishDate 2020-12-01
description A methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera <i>Desulfovibrio</i>, <i>Soehngenia</i>, <i>Thermovirga</i>, <i>Petrimonas</i>, <i>Methanosarcina</i>, and <i>Methanomethylovorans</i>. A novel gram-positive, rod-shaped, anaerobic fermentative bacterium, strain 1933P<sup>T</sup>, was isolated from this enrichment and characterized. The strain grew at 13–55 °C (optimum 35 °C), with 0–3.0% (<i>w</i>/<i>v</i>) NaCl (optimum 0–2.0%) and in the pH range of 6.7–8.0 (optimum pH 7.0). The 16S rRNA gene sequence similarity, the average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values between strain 1933P<sup>T</sup> and the type strain of the most closely related species <i>Soehngenia saccharolytica</i> DSM 12858<sup>T</sup> were 98.5%, 70.5%, and 22.6%, respectively, and were below the threshold accepted for species demarcation. Genome-based phylogenomic analysis and physiological and biochemical characterization of the strain 1933P<sup>T</sup> (VKM B-3382<sup>T</sup> = KCTC 15984<sup>T</sup>) confirmed its affiliation to a novel species of the genus <i>Soehngenia</i>, for which the name <i>Soehngenia longivitae</i> sp. nov. is proposed. Genome analysis suggests that the new strain has potential in the degradation of proteinaceous components.
topic <i>Soehngenia longivitae</i>
new species
genome analysis
polyphasic taxonomy
oilfield
url https://www.mdpi.com/2076-2607/8/12/1967
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